Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies
Abstract The human microbiome plays a crucial role in maintaining health, with advances in high‐throughput sequencing technology and reduced sequencing costs triggering a surge in microbiome research. Microbiome studies generally incorporate five key phases: design, sampling, sequencing, analysis, a...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14364 |
| _version_ | 1797333536533905408 |
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| author | Xin Yi Hong Lu Xiang Liu Junyi He Bing Li Zhelong Wang Yujing Zhao Xinri Zhang Xiao Yu |
| author_facet | Xin Yi Hong Lu Xiang Liu Junyi He Bing Li Zhelong Wang Yujing Zhao Xinri Zhang Xiao Yu |
| author_sort | Xin Yi |
| collection | DOAJ |
| description | Abstract The human microbiome plays a crucial role in maintaining health, with advances in high‐throughput sequencing technology and reduced sequencing costs triggering a surge in microbiome research. Microbiome studies generally incorporate five key phases: design, sampling, sequencing, analysis, and reporting, with sequencing strategy being a crucial step offering numerous options. Present mainstream sequencing strategies include Amplicon sequencing, Metagenomic Next‐Generation Sequencing (mNGS), and Targeted Next‐Generation Sequencing (tNGS). Two innovative technologies recently emerged, namely MobiMicrobe high‐throughput microbial single‐cell genome sequencing technology and 2bRAD‐M simplified metagenomic sequencing technology, compensate for the limitations of mainstream technologies, each boasting unique core strengths. This paper reviews the basic principles and processes of these three mainstream and two novel microbiological technologies, aiding readers in understanding the benefits and drawbacks of different technologies, thereby guiding the selection of the most suitable method for their research endeavours. |
| first_indexed | 2024-03-08T08:06:24Z |
| format | Article |
| id | doaj.art-38a32080ddbe4f47808ead1732808723 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-08T08:06:24Z |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-38a32080ddbe4f47808ead17328087232024-02-02T10:34:50ZengWileyMicrobial Biotechnology1751-79152024-01-01171n/an/a10.1111/1751-7915.14364Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologiesXin Yi0Hong Lu1Xiang Liu2Junyi He3Bing Li4Zhelong Wang5Yujing Zhao6Xinri Zhang7Xiao Yu8Department of Pharmacy Shanxi Medical University Taiyuan People's Republic of ChinaDepartment of Clinical laboratory The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaDepartment of Public Health Shanxi Medical University Taiyuan People's Republic of ChinaDepartment of Pharmacy Guangdong Pharmaceutical University Guangzhou People's Republic of ChinaNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaNHC Key Laboratory of Pneumoconiosis, Shanxi Key Laboratory of Respiratory Diseases, Department of Pulmonary and Critical Care Medicine The First Hospital of Shanxi Medical University Taiyuan People's Republic of ChinaAbstract The human microbiome plays a crucial role in maintaining health, with advances in high‐throughput sequencing technology and reduced sequencing costs triggering a surge in microbiome research. Microbiome studies generally incorporate five key phases: design, sampling, sequencing, analysis, and reporting, with sequencing strategy being a crucial step offering numerous options. Present mainstream sequencing strategies include Amplicon sequencing, Metagenomic Next‐Generation Sequencing (mNGS), and Targeted Next‐Generation Sequencing (tNGS). Two innovative technologies recently emerged, namely MobiMicrobe high‐throughput microbial single‐cell genome sequencing technology and 2bRAD‐M simplified metagenomic sequencing technology, compensate for the limitations of mainstream technologies, each boasting unique core strengths. This paper reviews the basic principles and processes of these three mainstream and two novel microbiological technologies, aiding readers in understanding the benefits and drawbacks of different technologies, thereby guiding the selection of the most suitable method for their research endeavours.https://doi.org/10.1111/1751-7915.14364 |
| spellingShingle | Xin Yi Hong Lu Xiang Liu Junyi He Bing Li Zhelong Wang Yujing Zhao Xinri Zhang Xiao Yu Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies Microbial Biotechnology |
| title | Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies |
| title_full | Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies |
| title_fullStr | Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies |
| title_full_unstemmed | Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies |
| title_short | Unravelling the enigma of the human microbiome: Evolution and selection of sequencing technologies |
| title_sort | unravelling the enigma of the human microbiome evolution and selection of sequencing technologies |
| url | https://doi.org/10.1111/1751-7915.14364 |
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