Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes
A significant lack of donor organs restricts the opportunity to obtain tissue-specific scaffolds for tissue-engineering technologies. One of the acceptable solutions is the development of decellularization protocols for a human donor pancreas unsuitable for transplantation. A protocol of obtaining a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/119 |
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| author | Victor I. Sevastianov Anna S. Ponomareva Natalia V. Baranova Lyudmila A. Kirsanova Yulia B. Basok Evgeniy A. Nemets Dmitry N. Kruglov Igor A. Miloserdov Sergey V. Gautier |
| author_facet | Victor I. Sevastianov Anna S. Ponomareva Natalia V. Baranova Lyudmila A. Kirsanova Yulia B. Basok Evgeniy A. Nemets Dmitry N. Kruglov Igor A. Miloserdov Sergey V. Gautier |
| author_sort | Victor I. Sevastianov |
| collection | DOAJ |
| description | A significant lack of donor organs restricts the opportunity to obtain tissue-specific scaffolds for tissue-engineering technologies. One of the acceptable solutions is the development of decellularization protocols for a human donor pancreas unsuitable for transplantation. A protocol of obtaining a biocompatible tissue-specific scaffold from decellularized fragments with pronounced human pancreas lipomatosis signs with preserved basic fibrillary proteins of a pancreatic tissue extracellular matrix was developed. The scaffold supports the adhesion and proliferation of human adipose derived stem cell (hADSCs) and prolongs the viability and insulin-producing function of pancreatic islets. Experiments conducted allow for the reliance on the prospects of using the donor pancreas unsuitable for transplantation in the technologies of tissue engineering and regenerative medicine, including the development of a tissue equivalent of a pancreas. |
| first_indexed | 2024-03-11T09:59:41Z |
| format | Article |
| id | doaj.art-38a6acd8db43479ba2c3dd03c7f21f2f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:59:41Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-38a6acd8db43479ba2c3dd03c7f21f2f2023-11-16T15:29:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124111910.3390/ijms24010119Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural ChangesVictor I. Sevastianov0Anna S. Ponomareva1Natalia V. Baranova2Lyudmila A. Kirsanova3Yulia B. Basok4Evgeniy A. Nemets5Dmitry N. Kruglov6Igor A. Miloserdov7Sergey V. Gautier8The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaThe Shumakov National Medical Research Center of Transplantology and Artificial Organs, 123182 Moscow, RussiaA significant lack of donor organs restricts the opportunity to obtain tissue-specific scaffolds for tissue-engineering technologies. One of the acceptable solutions is the development of decellularization protocols for a human donor pancreas unsuitable for transplantation. A protocol of obtaining a biocompatible tissue-specific scaffold from decellularized fragments with pronounced human pancreas lipomatosis signs with preserved basic fibrillary proteins of a pancreatic tissue extracellular matrix was developed. The scaffold supports the adhesion and proliferation of human adipose derived stem cell (hADSCs) and prolongs the viability and insulin-producing function of pancreatic islets. Experiments conducted allow for the reliance on the prospects of using the donor pancreas unsuitable for transplantation in the technologies of tissue engineering and regenerative medicine, including the development of a tissue equivalent of a pancreas.https://www.mdpi.com/1422-0067/24/1/119pancreasextracellular matrixdecellularizationtissue-specific scaffoldhuman adipose derived stem cellpancreatic islets |
| spellingShingle | Victor I. Sevastianov Anna S. Ponomareva Natalia V. Baranova Lyudmila A. Kirsanova Yulia B. Basok Evgeniy A. Nemets Dmitry N. Kruglov Igor A. Miloserdov Sergey V. Gautier Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes International Journal of Molecular Sciences pancreas extracellular matrix decellularization tissue-specific scaffold human adipose derived stem cell pancreatic islets |
| title | Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes |
| title_full | Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes |
| title_fullStr | Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes |
| title_full_unstemmed | Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes |
| title_short | Decellularization of Human Pancreatic Fragments with Pronounced Signs of Structural Changes |
| title_sort | decellularization of human pancreatic fragments with pronounced signs of structural changes |
| topic | pancreas extracellular matrix decellularization tissue-specific scaffold human adipose derived stem cell pancreatic islets |
| url | https://www.mdpi.com/1422-0067/24/1/119 |
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