The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds
Background and Aim: Onychomycosis or nail fungus infection has an increasing prevalence with many effects on patients’ social life and mental health dermatophytes, yeasts and non-dermatophyte molds are among the best known agents of fungal infections of nails. The purpose of this study was to determ...
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Tehran University of Medical Sciences
2015-11-01
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| Series: | پیاورد سلامت |
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| Online Access: | http://payavard.tums.ac.ir/article-1-5818-en.html |
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| author | Bahram Ahmadi Sasan Rezaei Farshad Hashemi Mahdi Zareei Hoda Deli Seyed Jamal Hashemi |
| author_facet | Bahram Ahmadi Sasan Rezaei Farshad Hashemi Mahdi Zareei Hoda Deli Seyed Jamal Hashemi |
| author_sort | Bahram Ahmadi |
| collection | DOAJ |
| description | Background and Aim: Onychomycosis or nail fungus infection has an increasing prevalence with many effects on patients’ social life and mental health dermatophytes, yeasts and non-dermatophyte molds are among the best known agents of fungal infections of nails. The purpose of this study was to determine the prevalence of non-dermatophyte molds using morphological (direct examination and culture) and molecular (PCR) methods in patients referring to Medical Sciences Mycology Laboratory in Tehran, Iran.
Materials and Methods: In this study, samples were taken from 170 patients. For direct microscopic examination (DME), 15% KOH solution was used for the culture of samples, Sabouraud dextrose agar media (S) was applied together with chloramphenicol (SC) and chloramphenicol and cycloheximide (SCC). Meanwhile, differential tests were done for mycological diagnosis (slide culture), and 28SrDNA amplification and sequencing were performed for suspect or unknown samples.
Results: Of the 170 patients, 74 cases (43.5%) had onychomycosis, of which 53 cases (71.62%) were female and 21 cases (28.38%) were male. Also, of the 74 cases of onychomycosis, 40 cases (54.05%) were reported candidiasis, 21 cases (28.37%) non-dermatophyte molds, and 12 cases (16.21 %) dermatophytes.
Conclusion: The prevalence of onychomycosis in this study was 43.5% and the application of polymerase chain reaction (PCR) technique in cases of false positive, false negative and long-term culture was valuable meanwhile, given that all the samples that had positive results in DME with negative cultures were positive in molecular tests, this study reveals the power of molecular techniques compared with culture method. |
| first_indexed | 2024-12-22T06:31:29Z |
| format | Article |
| id | doaj.art-38a7733b554841848303e19da6f78f80 |
| institution | Directory Open Access Journal |
| issn | 1735-8132 2008-2665 |
| language | fas |
| last_indexed | 2024-12-22T06:31:29Z |
| publishDate | 2015-11-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | پیاورد سلامت |
| spelling | doaj.art-38a7733b554841848303e19da6f78f802022-12-21T18:35:41ZfasTehran University of Medical Sciencesپیاورد سلامت1735-81322008-26652015-11-0194388399The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic MoldsBahram Ahmadi0Sasan Rezaei1Farshad Hashemi2Mahdi Zareei3Hoda Deli4Seyed Jamal Hashemi5 Ph.D Student in Medical Mycology, Parasitology & Medical Mycology Department, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran Professor, Parasitology & Medical Mycology Department, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran Pharmacy Student, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Ph.d Student in Medical Mycology, School of Medical Sciences, Tarbiat Moddares University, Tehran, Iran Master of Science Student in Medical Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran Professor, Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran Background and Aim: Onychomycosis or nail fungus infection has an increasing prevalence with many effects on patients’ social life and mental health dermatophytes, yeasts and non-dermatophyte molds are among the best known agents of fungal infections of nails. The purpose of this study was to determine the prevalence of non-dermatophyte molds using morphological (direct examination and culture) and molecular (PCR) methods in patients referring to Medical Sciences Mycology Laboratory in Tehran, Iran. Materials and Methods: In this study, samples were taken from 170 patients. For direct microscopic examination (DME), 15% KOH solution was used for the culture of samples, Sabouraud dextrose agar media (S) was applied together with chloramphenicol (SC) and chloramphenicol and cycloheximide (SCC). Meanwhile, differential tests were done for mycological diagnosis (slide culture), and 28SrDNA amplification and sequencing were performed for suspect or unknown samples. Results: Of the 170 patients, 74 cases (43.5%) had onychomycosis, of which 53 cases (71.62%) were female and 21 cases (28.38%) were male. Also, of the 74 cases of onychomycosis, 40 cases (54.05%) were reported candidiasis, 21 cases (28.37%) non-dermatophyte molds, and 12 cases (16.21 %) dermatophytes. Conclusion: The prevalence of onychomycosis in this study was 43.5% and the application of polymerase chain reaction (PCR) technique in cases of false positive, false negative and long-term culture was valuable meanwhile, given that all the samples that had positive results in DME with negative cultures were positive in molecular tests, this study reveals the power of molecular techniques compared with culture method.http://payavard.tums.ac.ir/article-1-5818-en.htmlonychomycosisnon-dermatophytedermatophytepcrmold |
| spellingShingle | Bahram Ahmadi Sasan Rezaei Farshad Hashemi Mahdi Zareei Hoda Deli Seyed Jamal Hashemi The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds پیاورد سلامت onychomycosis non-dermatophyte dermatophyte pcr mold |
| title | The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds |
| title_full | The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds |
| title_fullStr | The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds |
| title_full_unstemmed | The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds |
| title_short | The Use Of PCR To Diagnose Onychomycosis Caused By Non-Dermatophytic Molds |
| title_sort | use of pcr to diagnose onychomycosis caused by non dermatophytic molds |
| topic | onychomycosis non-dermatophyte dermatophyte pcr mold |
| url | http://payavard.tums.ac.ir/article-1-5818-en.html |
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