LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway
Acute liver injury is a common and serious syndrome caused by multiple factors and unclear pathogenesis. If the injury persists, liver injury can lead to cirrhosis and liver failure and ultimately results in the development of liver cancer. Emerging evidence has indicated that long noncoding RNAs (l...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2022-11-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2022167 |
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| author | Shao Shuai Zhang Yu Zhou Feng Meng Xiaoxiang Yu Zhenjun Li Guantong Zheng Lina Zhang Kun Li Yuhan Guo Beichen Liu Qi Zhang Mengxia Du Xiaoxiao Hong Wei Han Tao |
| author_facet | Shao Shuai Zhang Yu Zhou Feng Meng Xiaoxiang Yu Zhenjun Li Guantong Zheng Lina Zhang Kun Li Yuhan Guo Beichen Liu Qi Zhang Mengxia Du Xiaoxiao Hong Wei Han Tao |
| author_sort | Shao Shuai |
| collection | DOAJ |
| description | Acute liver injury is a common and serious syndrome caused by multiple factors and unclear pathogenesis. If the injury persists, liver injury can lead to cirrhosis and liver failure and ultimately results in the development of liver cancer. Emerging evidence has indicated that long noncoding RNAs (lncRNAs) play an important role in the development of liver injury. However, the role of antisense Igf2r RNA (Airn) in acute liver injury and its underlying mechanism remain largely unclear. In this study, we show that Airn is upregulated in liver tissue and primary hepatocytes from an acute liver injury mouse model. Consistently, Airn is also overexpressed in serum samples of patients with acute-on-chronic liver failure and is negatively correlated with the Model for End-Stage Liver Disease (MELD) score. Moreover, gene knockout and rescue assays reveal that Airn alleviates CCl <sub>4</sub>-induced liver injury by inhibiting hepatocyte apoptosis and oxidative stress in vivo. Further investigation reveals that Airn decreases H <sub>2</sub>O <sub>2</sub>-induced hepatocyte apoptosis in vitro. Mechanistically, we reveal that Airn represses CCl <sub>4</sub>- and H <sub>2</sub>O <sub>2</sub>-induced enhancement of phosphorylation of p65 and IκBα, suggesting that Airn inhibits hepatocyte apoptosis by inactivating the NF-κB pathway. In conclusion, our results demonstrate that Airn can alleviate acute liver injury by inhibiting hepatocyte apoptosis via inactivating the NF-κB signaling pathway, and Airn could be a potential biomarker for acute liver injury. |
| first_indexed | 2024-03-11T12:20:36Z |
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| id | doaj.art-38a7fdb8de064d5f803b29c49a01ec2f |
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| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:20:36Z |
| publishDate | 2022-11-01 |
| publisher | China Science Publishing & Media Ltd. |
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| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-38a7fdb8de064d5f803b29c49a01ec2f2023-11-07T01:02:29ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452022-11-01541619162910.3724/abbs.202216720d259ccLncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathwayShao Shuai0Zhang Yu1Zhou Feng2Meng Xiaoxiang3Yu Zhenjun4Li Guantong5Zheng Lina6Zhang Kun7Li Yuhan8Guo Beichen9Liu Qi10Zhang Mengxia11Du Xiaoxiao12Hong Wei13Han Tao14["The School of Medicine, Nankai University, Tianjin 300071, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China"]["The School of Medicine, Nankai University, Tianjin 300071, China","Department of Hepatology and Gastroenterology, the Third Central Clinical College of Tianjin Medical University; Department of Histology and Embryology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300121, China","Department of Gastroenterology and Hepatology, Tianjin Union Medical Center Affiliated to Nankai University, Tianjin 300122, China","Department of Hepatology and Gastroenterology, Tianjin Third Central Hospital Affiliated to Nankai University, Tianjin 300170, China"]Acute liver injury is a common and serious syndrome caused by multiple factors and unclear pathogenesis. If the injury persists, liver injury can lead to cirrhosis and liver failure and ultimately results in the development of liver cancer. Emerging evidence has indicated that long noncoding RNAs (lncRNAs) play an important role in the development of liver injury. However, the role of antisense Igf2r RNA (Airn) in acute liver injury and its underlying mechanism remain largely unclear. In this study, we show that Airn is upregulated in liver tissue and primary hepatocytes from an acute liver injury mouse model. Consistently, Airn is also overexpressed in serum samples of patients with acute-on-chronic liver failure and is negatively correlated with the Model for End-Stage Liver Disease (MELD) score. Moreover, gene knockout and rescue assays reveal that Airn alleviates CCl <sub>4</sub>-induced liver injury by inhibiting hepatocyte apoptosis and oxidative stress in vivo. Further investigation reveals that Airn decreases H <sub>2</sub>O <sub>2</sub>-induced hepatocyte apoptosis in vitro. Mechanistically, we reveal that Airn represses CCl <sub>4</sub>- and H <sub>2</sub>O <sub>2</sub>-induced enhancement of phosphorylation of p65 and IκBα, suggesting that Airn inhibits hepatocyte apoptosis by inactivating the NF-κB pathway. In conclusion, our results demonstrate that Airn can alleviate acute liver injury by inhibiting hepatocyte apoptosis via inactivating the NF-κB signaling pathway, and Airn could be a potential biomarker for acute liver injury. https://www.sciengine.com/doi/10.3724/abbs.2022167acute liver injurylncRNAapoptosisoxidative stressNF-κB signaling |
| spellingShingle | Shao Shuai Zhang Yu Zhou Feng Meng Xiaoxiang Yu Zhenjun Li Guantong Zheng Lina Zhang Kun Li Yuhan Guo Beichen Liu Qi Zhang Mengxia Du Xiaoxiao Hong Wei Han Tao LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway Acta Biochimica et Biophysica Sinica acute liver injury lncRNA apoptosis oxidative stress NF-κB signaling |
| title | LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway |
| title_full | LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway |
| title_fullStr | LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway |
| title_full_unstemmed | LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway |
| title_short | LncRNA-Airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the NF-κB signaling pathway |
| title_sort | lncrna airn alleviates acute liver injury by inhibiting hepatocyte apoptosis via the nf κb signaling pathway |
| topic | acute liver injury lncRNA apoptosis oxidative stress NF-κB signaling |
| url | https://www.sciengine.com/doi/10.3724/abbs.2022167 |
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