Linker, loading, and reaction scale influence automated glycan assembly
Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not signif...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Beilstein-Institut
2023-07-01
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| Series: | Beilstein Journal of Organic Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.3762/bjoc.19.77 |
| _version_ | 1797752322226388992 |
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| author | Marlene C. S. Dal Colle Manuel G. Ricardo Nives Hribernik José Danglad-Flores Peter H. Seeberger Martina Delbianco |
| author_facet | Marlene C. S. Dal Colle Manuel G. Ricardo Nives Hribernik José Danglad-Flores Peter H. Seeberger Martina Delbianco |
| author_sort | Marlene C. S. Dal Colle |
| collection | DOAJ |
| description | Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not significantly influence the AGA outcome, the chemical nature of the linker dramatically altered the isolated yields. We identified that the major determinants of AGA yields are cleavage from the solid support and post-AGA purification steps. |
| first_indexed | 2024-03-12T17:02:44Z |
| format | Article |
| id | doaj.art-38a83409005b429590dd4434f086a212 |
| institution | Directory Open Access Journal |
| issn | 1860-5397 |
| language | English |
| last_indexed | 2024-03-12T17:02:44Z |
| publishDate | 2023-07-01 |
| publisher | Beilstein-Institut |
| record_format | Article |
| series | Beilstein Journal of Organic Chemistry |
| spelling | doaj.art-38a83409005b429590dd4434f086a2122023-08-07T08:43:26ZengBeilstein-InstitutBeilstein Journal of Organic Chemistry1860-53972023-07-011911015102010.3762/bjoc.19.771860-5397-19-77Linker, loading, and reaction scale influence automated glycan assemblyMarlene C. S. Dal Colle0Manuel G. Ricardo1Nives Hribernik2José Danglad-Flores3Peter H. Seeberger4Martina Delbianco5Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany Automated glycan assembly (AGA) affords collections of well-defined glycans in a short amount of time. We systematically analyzed how parameters connected to the solid support affect the AGA outcome for three different glycan sequences. We showed that, while loading and reaction scale did not significantly influence the AGA outcome, the chemical nature of the linker dramatically altered the isolated yields. We identified that the major determinants of AGA yields are cleavage from the solid support and post-AGA purification steps.https://doi.org/10.3762/bjoc.19.77automated glycan assemblyphotocleavable linkerpolysaccharidessolid-phase synthesis |
| spellingShingle | Marlene C. S. Dal Colle Manuel G. Ricardo Nives Hribernik José Danglad-Flores Peter H. Seeberger Martina Delbianco Linker, loading, and reaction scale influence automated glycan assembly Beilstein Journal of Organic Chemistry automated glycan assembly photocleavable linker polysaccharides solid-phase synthesis |
| title | Linker, loading, and reaction scale influence automated glycan assembly |
| title_full | Linker, loading, and reaction scale influence automated glycan assembly |
| title_fullStr | Linker, loading, and reaction scale influence automated glycan assembly |
| title_full_unstemmed | Linker, loading, and reaction scale influence automated glycan assembly |
| title_short | Linker, loading, and reaction scale influence automated glycan assembly |
| title_sort | linker loading and reaction scale influence automated glycan assembly |
| topic | automated glycan assembly photocleavable linker polysaccharides solid-phase synthesis |
| url | https://doi.org/10.3762/bjoc.19.77 |
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