Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease
Alcohol-induced chronic liver disease (ALD) is becoming the most common liver disease in the world. However, there are no effective, universally accepted therapies for ALD. The etiology of ALD remains blurry so far. Historical evidence has demonstrated a link between the liver and gut microbiota. Bu...
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.00165/full |
| _version_ | 1818197010254135296 |
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| author | Hong Wang Yaping Yan Xiaoyan Yi Yanchao Duan Junfeng Wang Shanshan Li Lilin Luo Tianzhuang Huang Briauna Inglis Xi Li Weizhi Ji Tao Tan Wei Si |
| author_facet | Hong Wang Yaping Yan Xiaoyan Yi Yanchao Duan Junfeng Wang Shanshan Li Lilin Luo Tianzhuang Huang Briauna Inglis Xi Li Weizhi Ji Tao Tan Wei Si |
| author_sort | Hong Wang |
| collection | DOAJ |
| description | Alcohol-induced chronic liver disease (ALD) is becoming the most common liver disease in the world. However, there are no effective, universally accepted therapies for ALD. The etiology of ALD remains blurry so far. Historical evidence has demonstrated a link between the liver and gut microbiota. But it is difficult to distinguish the effect of gut microbiota changes caused by alcohol consumption in humans since the microbiota change detected in humans is complicated by diet and environmental factors. Due to the genetic, physiological, metabolic, and behavioral similarities to humans, the rhesus monkey provides excellent translational validity in preclinical studies, and the diet and environmental conditions can be controlled well in rhesus monkey. In our study, we explored the relationship between ALD and the gut microbiome in the rhesus monkeys with alcoholic liver steatosis. Our results showed that there was a change of the bacterial community structure in monkeys with ALD. Differences of the relative abundances of gut microbiota at phylum, order, family, genus, and species levels were observed between control monkeys and monkeys with ALD, and different pathways enriched in the monkeys with ALD were identified by metagenomic function analysis. Firmicutes, Proteobacteria, Verrucomicrobia tended to increase whereas Bacteroidetes and Actinobacteria decreased in the fecal microbiota of ALD group compared to the control group. Lactobacillales and Lactobacillus significantly decreased in ALD monkeys compared with normal monkeys, Streptococcus was lower in the ALD group compared with the control group. The non-human primate model of ALD will be useful for exploration of the microbiome markers as diagnosis and potentially prognosis for ALD. The ALD model will benefit the development of new therapeutic procedures for treating ALD and provide safety and efficacy evaluation for clinical application. |
| first_indexed | 2024-12-12T01:43:10Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T01:43:10Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-38a945db5d844a7c8b0b4f132bfbfdbf2022-12-22T00:42:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-02-011010.3389/fmicb.2019.00165417358Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver DiseaseHong Wang0Yaping Yan1Xiaoyan Yi2Yanchao Duan3Junfeng Wang4Shanshan Li5Lilin Luo6Tianzhuang Huang7Briauna Inglis8Xi Li9Weizhi Ji10Tao Tan11Wei Si12Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaShenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaDepartment of Hepatic and Bile Duct Surgery, The First People's Hospital of Yunnan Province, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaDepartment of Pathology, The First People's Hospital of Yunnan Province, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, ChinaAlcohol-induced chronic liver disease (ALD) is becoming the most common liver disease in the world. However, there are no effective, universally accepted therapies for ALD. The etiology of ALD remains blurry so far. Historical evidence has demonstrated a link between the liver and gut microbiota. But it is difficult to distinguish the effect of gut microbiota changes caused by alcohol consumption in humans since the microbiota change detected in humans is complicated by diet and environmental factors. Due to the genetic, physiological, metabolic, and behavioral similarities to humans, the rhesus monkey provides excellent translational validity in preclinical studies, and the diet and environmental conditions can be controlled well in rhesus monkey. In our study, we explored the relationship between ALD and the gut microbiome in the rhesus monkeys with alcoholic liver steatosis. Our results showed that there was a change of the bacterial community structure in monkeys with ALD. Differences of the relative abundances of gut microbiota at phylum, order, family, genus, and species levels were observed between control monkeys and monkeys with ALD, and different pathways enriched in the monkeys with ALD were identified by metagenomic function analysis. Firmicutes, Proteobacteria, Verrucomicrobia tended to increase whereas Bacteroidetes and Actinobacteria decreased in the fecal microbiota of ALD group compared to the control group. Lactobacillales and Lactobacillus significantly decreased in ALD monkeys compared with normal monkeys, Streptococcus was lower in the ALD group compared with the control group. The non-human primate model of ALD will be useful for exploration of the microbiome markers as diagnosis and potentially prognosis for ALD. The ALD model will benefit the development of new therapeutic procedures for treating ALD and provide safety and efficacy evaluation for clinical application.https://www.frontiersin.org/article/10.3389/fmicb.2019.00165/fullgut microbiotahistopathological featuresalcoholic liver diseasemetagenomic sequencingrhesus monkey |
| spellingShingle | Hong Wang Yaping Yan Xiaoyan Yi Yanchao Duan Junfeng Wang Shanshan Li Lilin Luo Tianzhuang Huang Briauna Inglis Xi Li Weizhi Ji Tao Tan Wei Si Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease Frontiers in Microbiology gut microbiota histopathological features alcoholic liver disease metagenomic sequencing rhesus monkey |
| title | Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease |
| title_full | Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease |
| title_fullStr | Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease |
| title_full_unstemmed | Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease |
| title_short | Histopathological Features and Composition of Gut Microbiota in Rhesus Monkey of Alcoholic Liver Disease |
| title_sort | histopathological features and composition of gut microbiota in rhesus monkey of alcoholic liver disease |
| topic | gut microbiota histopathological features alcoholic liver disease metagenomic sequencing rhesus monkey |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.00165/full |
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