Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham.
The objective of the present work was to evaluate the efficacy of use of the RITA® temporary immersion system in the large-scale propagation of P. patula. The effects of four concentrations (0.00 µM, 4.50 µM, 9.00 µM, and 13.51 µM) of 6-benzylaminopurine (BAP) on 10 hypocotyl explants were studied u...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2023-03-01
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| Series: | Journal of Forest Science |
| Subjects: | |
| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-202303-0004_use-of-bioreactors-rita-174-in-the-propagation-of-pinus-patula-schiede-ex-schltdl-amp-cham.php |
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| author | Arturo Alonso Armas Silva Lourdes Georgina Iglesias Andreu Marco Antonio Ramírez Mosqueda |
| author_facet | Arturo Alonso Armas Silva Lourdes Georgina Iglesias Andreu Marco Antonio Ramírez Mosqueda |
| author_sort | Arturo Alonso Armas Silva |
| collection | DOAJ |
| description | The objective of the present work was to evaluate the efficacy of use of the RITA® temporary immersion system in the large-scale propagation of P. patula. The effects of four concentrations (0.00 µM, 4.50 µM, 9.00 µM, and 13.51 µM) of 6-benzylaminopurine (BAP) on 10 hypocotyl explants were studied using a completely randomised design with three replicates per treatment. Five hypocotyl explants were grown in 250 mL RITA® containers of Woody Plant Culture Medium (WPM) supplemented with 20 g.L-1 sucrose and 10 mg.L-1 vitamins from Murashige and Skoog (MS) culture medium. The frequency of immersion of the explants into the culture medium was 2 min every 8 hours. The number of adventitious buds and calli formed, as well as shoot growth, were evaluated after 6 weeks of in vitro culture. The 4.50 µM concentration of BAP was the best treatment for shoot production (5 shoots per plant) and shoot length (1.32 cm). These results could help the widespread vegetative propagation of this important forest species. |
| first_indexed | 2024-04-09T21:01:40Z |
| format | Article |
| id | doaj.art-38ab4db3ed31425e8efa0ba49dd9fc85 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-09T21:01:40Z |
| publishDate | 2023-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-38ab4db3ed31425e8efa0ba49dd9fc852023-03-29T08:10:37ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2023-03-0169312412610.17221/189/2022-JFSjfs-202303-0004Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham.Arturo Alonso Armas Silva0Lourdes Georgina Iglesias Andreu1Marco Antonio Ramírez Mosqueda2Institute of Biotechnology and Applied Ecology (INBIOTECA), Veracruz University, Veracruz, MexicoInstitute of Biotechnology and Applied Ecology (INBIOTECA), Veracruz University, Veracruz, MexicoFaculty of Biological and Agricultural Sciences, Veracruz University, Veracruz, MexicoThe objective of the present work was to evaluate the efficacy of use of the RITA® temporary immersion system in the large-scale propagation of P. patula. The effects of four concentrations (0.00 µM, 4.50 µM, 9.00 µM, and 13.51 µM) of 6-benzylaminopurine (BAP) on 10 hypocotyl explants were studied using a completely randomised design with three replicates per treatment. Five hypocotyl explants were grown in 250 mL RITA® containers of Woody Plant Culture Medium (WPM) supplemented with 20 g.L-1 sucrose and 10 mg.L-1 vitamins from Murashige and Skoog (MS) culture medium. The frequency of immersion of the explants into the culture medium was 2 min every 8 hours. The number of adventitious buds and calli formed, as well as shoot growth, were evaluated after 6 weeks of in vitro culture. The 4.50 µM concentration of BAP was the best treatment for shoot production (5 shoots per plant) and shoot length (1.32 cm). These results could help the widespread vegetative propagation of this important forest species.https://jfs.agriculturejournals.cz/artkey/jfs-202303-0004_use-of-bioreactors-rita-174-in-the-propagation-of-pinus-patula-schiede-ex-schltdl-amp-cham.phpconifers in vitromicropropagationtemporary immersion systemrecipient for automated temporary immersion |
| spellingShingle | Arturo Alonso Armas Silva Lourdes Georgina Iglesias Andreu Marco Antonio Ramírez Mosqueda Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. Journal of Forest Science conifers in vitro micropropagation temporary immersion system recipient for automated temporary immersion |
| title | Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. |
| title_full | Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. |
| title_fullStr | Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. |
| title_full_unstemmed | Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. |
| title_short | Use of bioreactors RITA® in the propagation of Pinus patula Schiede ex Schltdl. & Cham. |
| title_sort | use of bioreactors rita r in the propagation of pinus patula schiede ex schltdl cham |
| topic | conifers in vitro micropropagation temporary immersion system recipient for automated temporary immersion |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-202303-0004_use-of-bioreactors-rita-174-in-the-propagation-of-pinus-patula-schiede-ex-schltdl-amp-cham.php |
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