Mouse embryo phenotyping using X-ray microCT
Microscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlativ...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2022-09-01
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Series: | Frontiers in Cell and Developmental Biology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/full |
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author | Stephan Handschuh Martin Glösmann |
author_facet | Stephan Handschuh Martin Glösmann |
author_sort | Stephan Handschuh |
collection | DOAJ |
description | Microscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlative imaging workflows. Protocols for staining embryos with X-ray dense contrast agents enable the acquisition of high-contrast and high-resolution datasets of whole embryos and specific organ systems. High sample throughput is achieved with dedicated setups. Consequently, microCT has gained enormous importance for both qualitative and quantitative phenotyping of mouse development. We here summarize state-of-the-art protocols of sample preparation and imaging procedures, showcase contemporary applications, and discuss possible pitfalls and sources for artefacts. In addition, we give an outlook on phenotyping workflows using microscopic dual energy CT (microDECT) and tissue-specific contrast agents. |
first_indexed | 2024-04-11T20:25:47Z |
format | Article |
id | doaj.art-38ace70f83254ab2b6b2a1d670bddfcd |
institution | Directory Open Access Journal |
issn | 2296-634X |
language | English |
last_indexed | 2024-04-11T20:25:47Z |
publishDate | 2022-09-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Cell and Developmental Biology |
spelling | doaj.art-38ace70f83254ab2b6b2a1d670bddfcd2022-12-22T04:04:40ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.949184949184Mouse embryo phenotyping using X-ray microCTStephan HandschuhMartin GlösmannMicroscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlative imaging workflows. Protocols for staining embryos with X-ray dense contrast agents enable the acquisition of high-contrast and high-resolution datasets of whole embryos and specific organ systems. High sample throughput is achieved with dedicated setups. Consequently, microCT has gained enormous importance for both qualitative and quantitative phenotyping of mouse development. We here summarize state-of-the-art protocols of sample preparation and imaging procedures, showcase contemporary applications, and discuss possible pitfalls and sources for artefacts. In addition, we give an outlook on phenotyping workflows using microscopic dual energy CT (microDECT) and tissue-specific contrast agents.https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/fullμCTX ray microtomographygrowth and developmentlaboratory animal modelsfetus |
spellingShingle | Stephan Handschuh Martin Glösmann Mouse embryo phenotyping using X-ray microCT Frontiers in Cell and Developmental Biology μCT X ray microtomography growth and development laboratory animal models fetus |
title | Mouse embryo phenotyping using X-ray microCT |
title_full | Mouse embryo phenotyping using X-ray microCT |
title_fullStr | Mouse embryo phenotyping using X-ray microCT |
title_full_unstemmed | Mouse embryo phenotyping using X-ray microCT |
title_short | Mouse embryo phenotyping using X-ray microCT |
title_sort | mouse embryo phenotyping using x ray microct |
topic | μCT X ray microtomography growth and development laboratory animal models fetus |
url | https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/full |
work_keys_str_mv | AT stephanhandschuh mouseembryophenotypingusingxraymicroct AT martinglosmann mouseembryophenotypingusingxraymicroct |