Mouse embryo phenotyping using X-ray microCT

Microscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlativ...

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Main Authors: Stephan Handschuh, Martin Glösmann
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/full
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author Stephan Handschuh
Martin Glösmann
author_facet Stephan Handschuh
Martin Glösmann
author_sort Stephan Handschuh
collection DOAJ
description Microscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlative imaging workflows. Protocols for staining embryos with X-ray dense contrast agents enable the acquisition of high-contrast and high-resolution datasets of whole embryos and specific organ systems. High sample throughput is achieved with dedicated setups. Consequently, microCT has gained enormous importance for both qualitative and quantitative phenotyping of mouse development. We here summarize state-of-the-art protocols of sample preparation and imaging procedures, showcase contemporary applications, and discuss possible pitfalls and sources for artefacts. In addition, we give an outlook on phenotyping workflows using microscopic dual energy CT (microDECT) and tissue-specific contrast agents.
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spelling doaj.art-38ace70f83254ab2b6b2a1d670bddfcd2022-12-22T04:04:40ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-09-011010.3389/fcell.2022.949184949184Mouse embryo phenotyping using X-ray microCTStephan HandschuhMartin GlösmannMicroscopic X-ray computed tomography (microCT) is a structural ex vivo imaging technique providing genuine isotropic 3D images from biological samples at micron resolution. MicroCT imaging is non-destructive and combines well with other modalities such as light and electron microscopy in correlative imaging workflows. Protocols for staining embryos with X-ray dense contrast agents enable the acquisition of high-contrast and high-resolution datasets of whole embryos and specific organ systems. High sample throughput is achieved with dedicated setups. Consequently, microCT has gained enormous importance for both qualitative and quantitative phenotyping of mouse development. We here summarize state-of-the-art protocols of sample preparation and imaging procedures, showcase contemporary applications, and discuss possible pitfalls and sources for artefacts. In addition, we give an outlook on phenotyping workflows using microscopic dual energy CT (microDECT) and tissue-specific contrast agents.https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/fullμCTX ray microtomographygrowth and developmentlaboratory animal modelsfetus
spellingShingle Stephan Handschuh
Martin Glösmann
Mouse embryo phenotyping using X-ray microCT
Frontiers in Cell and Developmental Biology
μCT
X ray microtomography
growth and development
laboratory animal models
fetus
title Mouse embryo phenotyping using X-ray microCT
title_full Mouse embryo phenotyping using X-ray microCT
title_fullStr Mouse embryo phenotyping using X-ray microCT
title_full_unstemmed Mouse embryo phenotyping using X-ray microCT
title_short Mouse embryo phenotyping using X-ray microCT
title_sort mouse embryo phenotyping using x ray microct
topic μCT
X ray microtomography
growth and development
laboratory animal models
fetus
url https://www.frontiersin.org/articles/10.3389/fcell.2022.949184/full
work_keys_str_mv AT stephanhandschuh mouseembryophenotypingusingxraymicroct
AT martinglosmann mouseembryophenotypingusingxraymicroct