Establishment of event-specific quantitative real-time polymerase chain reaction for detecting genetically modified sugarbeet GTSB77

Establishment of event-specific quantitative real-time polymerase chain reaction for detecting genetically modified sugarbeet GTSB77

Objective For implementation of labeling regulations, an event-specific real-time polymerase chain reaction (PCR) method for quantitative detection of sugarbeet GTSB77 was established. Methods The specific primer pairs and probe based on the 3’flanking sequence of GTSB77 were designed. The specifici...

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Bibliographic Details
Main Authors: Erlong LIU, Li LU, Yingzi LYU, Xiang JIANG, Jiaqi LI, Roufei XIA
Format: Article
Language:zho
Published: The Editorial Office of Chinese Journal of Food Hygiene 2020-01-01
Series:Zhongguo shipin weisheng zazhi
Subjects:
sugarbeet gtsb77
real-time polymerase chain reaction
event-specific
Online Access:http://www.zgspws.com/zgspwszzen/article/abstract/20200109
Description
Summary:Objective For implementation of labeling regulations, an event-specific real-time polymerase chain reaction (PCR) method for quantitative detection of sugarbeet GTSB77 was established. Methods The specific primer pairs and probe based on the 3’flanking sequence of GTSB77 were designed. The specificity, sensitivity and repeatability of the developed method were examined, respectively. Results The specificity test showed it specific for GTSB77 detection. The limit of quantification (LOQ) was 16 copies and the amplification efficiency was 102%. Reapeatability of the established method was assessed and the relative standard deviation (RSD) ranged from 0.21%-1.66%. Conclusion This event-specific real-time PCR method is suitable for identification of GTSB77.
ISSN:1004-8456

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