Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi
Pak choi is one of the most important leafy vegetables planted in East Asia and provides essential nutrients for the human body. Purple pak choi differs mainly in leaf colour but exhibits distinct nutritional profiles from green pak choi. In this study, we performed metabolic and transcriptomic anal...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13781 |
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| author | Jinglei Wang Tianhua Hu Yidi Wang Wuhong Wang Haijiao Hu Qingzhen Wei Yaqin Yan Chonglai Bao |
| author_facet | Jinglei Wang Tianhua Hu Yidi Wang Wuhong Wang Haijiao Hu Qingzhen Wei Yaqin Yan Chonglai Bao |
| author_sort | Jinglei Wang |
| collection | DOAJ |
| description | Pak choi is one of the most important leafy vegetables planted in East Asia and provides essential nutrients for the human body. Purple pak choi differs mainly in leaf colour but exhibits distinct nutritional profiles from green pak choi. In this study, we performed metabolic and transcriptomic analyses to uncover the mechanisms underlying the differences in metabolite biosynthesis profiles between the two pak choi varieties. Metabolite profiling revealed significant differences in the levels of metabolites, mainly amino acids and their derivatives and flavonoids. Furthermore, 34 flavonoids significantly differed between green and purple pak choi leaves, and cyanidin and its derivative anthocyanins were abundant in purple pak choi. In addition, we found that the structural genes <i>CHS</i>, <i>DFR</i>, <i>ANS</i>, and <i>UGT75C1</i>, as well as the transcription factor <i>MYB2</i>, play a major role in anthocyanin synthesis. These results provide insight into the molecular mechanisms underlying leaf pigmentation in pak choi and offer a platform for assessing related varieties. |
| first_indexed | 2024-03-10T22:41:04Z |
| format | Article |
| id | doaj.art-38af9e5107d14aca96e7a3a17825ad83 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:41:04Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-38af9e5107d14aca96e7a3a17825ad832023-11-19T11:03:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181378110.3390/ijms241813781Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak ChoiJinglei Wang0Tianhua Hu1Yidi Wang2Wuhong Wang3Haijiao Hu4Qingzhen Wei5Yaqin Yan6Chonglai Bao7Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaPak choi is one of the most important leafy vegetables planted in East Asia and provides essential nutrients for the human body. Purple pak choi differs mainly in leaf colour but exhibits distinct nutritional profiles from green pak choi. In this study, we performed metabolic and transcriptomic analyses to uncover the mechanisms underlying the differences in metabolite biosynthesis profiles between the two pak choi varieties. Metabolite profiling revealed significant differences in the levels of metabolites, mainly amino acids and their derivatives and flavonoids. Furthermore, 34 flavonoids significantly differed between green and purple pak choi leaves, and cyanidin and its derivative anthocyanins were abundant in purple pak choi. In addition, we found that the structural genes <i>CHS</i>, <i>DFR</i>, <i>ANS</i>, and <i>UGT75C1</i>, as well as the transcription factor <i>MYB2</i>, play a major role in anthocyanin synthesis. These results provide insight into the molecular mechanisms underlying leaf pigmentation in pak choi and offer a platform for assessing related varieties.https://www.mdpi.com/1422-0067/24/18/13781pak choitranscriptomesmetabolismanthocyanin<i>MYB2</i> |
| spellingShingle | Jinglei Wang Tianhua Hu Yidi Wang Wuhong Wang Haijiao Hu Qingzhen Wei Yaqin Yan Chonglai Bao Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi International Journal of Molecular Sciences pak choi transcriptomes metabolism anthocyanin <i>MYB2</i> |
| title | Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi |
| title_full | Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi |
| title_fullStr | Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi |
| title_full_unstemmed | Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi |
| title_short | Metabolic and Transcriptomic Analyses Reveal Different Metabolite Biosynthesis Profiles between Purple and Green Pak Choi |
| title_sort | metabolic and transcriptomic analyses reveal different metabolite biosynthesis profiles between purple and green pak choi |
| topic | pak choi transcriptomes metabolism anthocyanin <i>MYB2</i> |
| url | https://www.mdpi.com/1422-0067/24/18/13781 |
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