Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i>
The galling insect <i>Leptocybe invasa</i> Fisher & La Salle (Hymenoptera: Eulophidae) is a major limiting factor in the cultivation of drought-tolerant eucalyptus. The insect <i>L. invasa</i> is a major pest of eucalyptus plantations, and <i>Beauveria bassiana</...
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/1999-4907/14/7/1349 |
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| author | João Pedro Laurindo Rocha Thomas Vieira Nunes Jovielly Neves Rodrigues Nívea Maria Pereira Lima Pedro Augusto Laurindo Rocha Ismael de Oliveira Pinto Maíra Ignacio Sarmento Wagner L. Araújo Cristiano Bueno de Moraes Renato Almeida Sarmento |
| author_facet | João Pedro Laurindo Rocha Thomas Vieira Nunes Jovielly Neves Rodrigues Nívea Maria Pereira Lima Pedro Augusto Laurindo Rocha Ismael de Oliveira Pinto Maíra Ignacio Sarmento Wagner L. Araújo Cristiano Bueno de Moraes Renato Almeida Sarmento |
| author_sort | João Pedro Laurindo Rocha |
| collection | DOAJ |
| description | The galling insect <i>Leptocybe invasa</i> Fisher & La Salle (Hymenoptera: Eulophidae) is a major limiting factor in the cultivation of drought-tolerant eucalyptus. The insect <i>L. invasa</i> is a major pest of eucalyptus plantations, and <i>Beauveria bassiana</i> (Bals.) Vuill (Ascomycota: Hypocreales) is being investigated as a potential biocontrol agent against this pest. The fungus <i>B. bassiana</i> can produce metabolites that affect insect biology and survival. Here, we investigated the ability of the entomopathogenic <i>B. bassiana</i> to endophytically develop and induce resistance to <i>L. invasa</i> in a drought-tolerant eucalyptus hybrid. In a greenhouse under semi-controlled conditions, a group of seedlings were sprayed with a solution containing the fungal spores of <i>B. bassiana</i>. The uninoculated seedlings and seedlings inoculated were infested with <i>L. invasa</i>, and their morphometric responses, gas exchange, and chlorophyll indexes were assessed. The number of leaves and height of the inoculated plants was higher than those of the uninoculated plants. The mean CO<sub>2</sub> assimilation rate (A) and transpiration rate (E) were higher for inoculated plants. The inoculated plants showed higher chl a and chl b contents. Compared to the uninoculated plants, the inoculated plants developed much fewer galls, while some showed only scar formations where <i>L. invasa</i> deposited its eggs. These results indicated that inoculating <i>Eucalyptus</i> with <i>B. bassiana</i> promoted resistance to <i>L. invasa</i>. To the best of our knowledge, this is the first study showing that an entomopathogenic fungus can develop endophytically to promote resistance against a galling insect pest. |
| first_indexed | 2024-03-11T01:03:30Z |
| format | Article |
| id | doaj.art-38b11010c3994fb6adbb244e7b361194 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T01:03:30Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-38b11010c3994fb6adbb244e7b3611942023-11-18T19:22:59ZengMDPI AGForests1999-49072023-06-01147134910.3390/f14071349Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i>João Pedro Laurindo Rocha0Thomas Vieira Nunes1Jovielly Neves Rodrigues2Nívea Maria Pereira Lima3Pedro Augusto Laurindo Rocha4Ismael de Oliveira Pinto5Maíra Ignacio Sarmento6Wagner L. Araújo7Cristiano Bueno de Moraes8Renato Almeida Sarmento9Plant Physiology Graduate Program, Department of Plant Biology, Federal University of Viçosa (UFV), Viçosa 36570-900, Minas Gerais, BrazilProgram of Graduate Studies in Biotechnology and Biodiversity, Rede Bionorte, Universidade Federal do Tocantins (UFT), Campus Palmas, Palmas 77650-000, Tocantins, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilProgram of Graduate Studies in Plant Production, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilPlant Physiology Graduate Program, Department of Plant Biology, Federal University of Viçosa (UFV), Viçosa 36570-900, Minas Gerais, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilProgram of Graduate Studies in Forestry and Environmental Sciences, Universidade Federal do Tocantins (UFT), Campus Gurupi, Gurupi 77402-970, Tocantins, BrazilThe galling insect <i>Leptocybe invasa</i> Fisher & La Salle (Hymenoptera: Eulophidae) is a major limiting factor in the cultivation of drought-tolerant eucalyptus. The insect <i>L. invasa</i> is a major pest of eucalyptus plantations, and <i>Beauveria bassiana</i> (Bals.) Vuill (Ascomycota: Hypocreales) is being investigated as a potential biocontrol agent against this pest. The fungus <i>B. bassiana</i> can produce metabolites that affect insect biology and survival. Here, we investigated the ability of the entomopathogenic <i>B. bassiana</i> to endophytically develop and induce resistance to <i>L. invasa</i> in a drought-tolerant eucalyptus hybrid. In a greenhouse under semi-controlled conditions, a group of seedlings were sprayed with a solution containing the fungal spores of <i>B. bassiana</i>. The uninoculated seedlings and seedlings inoculated were infested with <i>L. invasa</i>, and their morphometric responses, gas exchange, and chlorophyll indexes were assessed. The number of leaves and height of the inoculated plants was higher than those of the uninoculated plants. The mean CO<sub>2</sub> assimilation rate (A) and transpiration rate (E) were higher for inoculated plants. The inoculated plants showed higher chl a and chl b contents. Compared to the uninoculated plants, the inoculated plants developed much fewer galls, while some showed only scar formations where <i>L. invasa</i> deposited its eggs. These results indicated that inoculating <i>Eucalyptus</i> with <i>B. bassiana</i> promoted resistance to <i>L. invasa</i>. To the best of our knowledge, this is the first study showing that an entomopathogenic fungus can develop endophytically to promote resistance against a galling insect pest.https://www.mdpi.com/1999-4907/14/7/1349fungal ecologyendophytic developmentpest controlinduced resistance |
| spellingShingle | João Pedro Laurindo Rocha Thomas Vieira Nunes Jovielly Neves Rodrigues Nívea Maria Pereira Lima Pedro Augusto Laurindo Rocha Ismael de Oliveira Pinto Maíra Ignacio Sarmento Wagner L. Araújo Cristiano Bueno de Moraes Renato Almeida Sarmento Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> Forests fungal ecology endophytic development pest control induced resistance |
| title | Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> |
| title_full | Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> |
| title_fullStr | Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> |
| title_full_unstemmed | Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> |
| title_short | Morphophysiological Responses in <i>Eucalyptus</i> Demonstrate the Potential of the Entomopathogenic Fungus <i>Beauveria bassiana</i> to Promote Resistance against the Galling Wasp <i>Leptocybe invasa</i> |
| title_sort | morphophysiological responses in i eucalyptus i demonstrate the potential of the entomopathogenic fungus i beauveria bassiana i to promote resistance against the galling wasp i leptocybe invasa i |
| topic | fungal ecology endophytic development pest control induced resistance |
| url | https://www.mdpi.com/1999-4907/14/7/1349 |
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