Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications
Mesoporous bioactive glass nanoparticles (MBGNPs) are attracting significant attention as suitable materials for multifunctional biomedical applications. In this study, cerium was incorporated into MBGNPs using two different approaches. In the first approach, cerium was added to the glass system dir...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006421000582 |
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| author | F. Kurtuldu H. Kaňková A.M. Beltrán L. Liverani D. Galusek A.R. Boccaccini |
| author_facet | F. Kurtuldu H. Kaňková A.M. Beltrán L. Liverani D. Galusek A.R. Boccaccini |
| author_sort | F. Kurtuldu |
| collection | DOAJ |
| description | Mesoporous bioactive glass nanoparticles (MBGNPs) are attracting significant attention as suitable materials for multifunctional biomedical applications. In this study, cerium was incorporated into MBGNPs using two different approaches. In the first approach, cerium was added to the glass system directly during the synthesis, while in the second approach, cerium was added to the as-synthesized MBGNPs via the template ion-exchange method. The influence of the method of synthesis on the physicochemical properties of nanoparticles was examined by SEM, TEM, XRD, FTIR, and N2 adsorption-desorption analyses. The MBGNPs exhibited spheroidal morphology and disordered mesoporous structure. XRD analysis confirmed the amorphous nature of the nanoparticles. The chemical composition was determined by the acid digestion method using ICP-OES. The influence of the synthesis method on the specific surface area, mesoporosity, and solubility of synthesized nanoparticles in Tris/HCl (pH 7.4) and acetate (pH 4.5) buffer has also been studied. The obtained Ce containing MBGNPs were non-cytotoxic toward preosteoblast MC3T3-E1 cells in contact with nanoparticles in a concentration of up to 100 μg/mL. The anti-inflammatory effect of Ce containing MBGNPs was tested with lipopolysaccharides (LPS)-induced proinflammatory RAW 264.7 macrophage cells. Ce containing MBGNPs decreased the release of nitric oxide, indicating the anti-inflammatory response of macrophage cells. Ce containing MBGNPs also showed antibacterial activity against S. aureus and E. coli. The mentioned features of the obtained MBGNPs make them useful in a variety of biomedical applications, considering their biocompatibility, anti-inflammatory response, and enhanced antibacterial effect. |
| first_indexed | 2024-12-22T01:26:42Z |
| format | Article |
| id | doaj.art-38b260b23a4e4381aa078944e3d2c450 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-12-22T01:26:42Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-38b260b23a4e4381aa078944e3d2c4502022-12-21T18:43:36ZengElsevierMaterials Today Bio2590-00642021-09-0112100150Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applicationsF. Kurtuldu0H. Kaňková1A.M. Beltrán2L. Liverani3D. Galusek4A.R. Boccaccini5FunGlass, Alexander Dubček University of Trenčín, 911 50 Trenčín, Slovakia; Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyFunGlass, Alexander Dubček University of Trenčín, 911 50 Trenčín, SlovakiaDepartamento de Ingeniería y Ciencia de Los Materiales y Del Transporte, Escuela Politécnica Superior, University of Seville, 41011 Seville, SpainInstitute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, GermanyFunGlass, Alexander Dubček University of Trenčín, 911 50 Trenčín, Slovakia; Joint Glass Centre of the IIC SAS, TnUAD and FChFT STU, FunGlass, 911 50, Trenčín, Slovakia; Corresponding author.Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, 91058 Erlangen, Germany; Corresponding author.Mesoporous bioactive glass nanoparticles (MBGNPs) are attracting significant attention as suitable materials for multifunctional biomedical applications. In this study, cerium was incorporated into MBGNPs using two different approaches. In the first approach, cerium was added to the glass system directly during the synthesis, while in the second approach, cerium was added to the as-synthesized MBGNPs via the template ion-exchange method. The influence of the method of synthesis on the physicochemical properties of nanoparticles was examined by SEM, TEM, XRD, FTIR, and N2 adsorption-desorption analyses. The MBGNPs exhibited spheroidal morphology and disordered mesoporous structure. XRD analysis confirmed the amorphous nature of the nanoparticles. The chemical composition was determined by the acid digestion method using ICP-OES. The influence of the synthesis method on the specific surface area, mesoporosity, and solubility of synthesized nanoparticles in Tris/HCl (pH 7.4) and acetate (pH 4.5) buffer has also been studied. The obtained Ce containing MBGNPs were non-cytotoxic toward preosteoblast MC3T3-E1 cells in contact with nanoparticles in a concentration of up to 100 μg/mL. The anti-inflammatory effect of Ce containing MBGNPs was tested with lipopolysaccharides (LPS)-induced proinflammatory RAW 264.7 macrophage cells. Ce containing MBGNPs decreased the release of nitric oxide, indicating the anti-inflammatory response of macrophage cells. Ce containing MBGNPs also showed antibacterial activity against S. aureus and E. coli. The mentioned features of the obtained MBGNPs make them useful in a variety of biomedical applications, considering their biocompatibility, anti-inflammatory response, and enhanced antibacterial effect.http://www.sciencedirect.com/science/article/pii/S2590006421000582Metal ionBioactive glasssesNanoparticlesAnti-inflammation activityAntibacterial materials |
| spellingShingle | F. Kurtuldu H. Kaňková A.M. Beltrán L. Liverani D. Galusek A.R. Boccaccini Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications Materials Today Bio Metal ion Bioactive glassses Nanoparticles Anti-inflammation activity Antibacterial materials |
| title | Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications |
| title_full | Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications |
| title_fullStr | Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications |
| title_full_unstemmed | Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications |
| title_short | Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications |
| title_sort | anti inflammatory and antibacterial activities of cerium containing mesoporous bioactive glass nanoparticles for drug free biomedical applications |
| topic | Metal ion Bioactive glassses Nanoparticles Anti-inflammation activity Antibacterial materials |
| url | http://www.sciencedirect.com/science/article/pii/S2590006421000582 |
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