3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control
OBJECTIVES/SPECIFIC AIMS: Our objective was to compare the proteomics of HDL between youth with T1DM and healthy controls (HC). METHODS/STUDY POPULATION: We did chromatography-based HDL purification and SWATH-MS-based proteomic quantitation. Proteomic alterations of HDL fractions and their associati...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2019-03-01
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| Series: | Journal of Clinical and Translational Science |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2059866119000761/type/journal_article |
| _version_ | 1811156630450995200 |
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| author | Evgenia Gourgari Scott Gordon Junfeng Ma Martin Playford Nehal Mehta Radoslav Goldman Alan Remaley |
| author_facet | Evgenia Gourgari Scott Gordon Junfeng Ma Martin Playford Nehal Mehta Radoslav Goldman Alan Remaley |
| author_sort | Evgenia Gourgari |
| collection | DOAJ |
| description | OBJECTIVES/SPECIFIC AIMS: Our objective was to compare the proteomics of HDL between youth with T1DM and healthy controls (HC). METHODS/STUDY POPULATION: We did chromatography-based HDL purification and SWATH-MS-based proteomic quantitation. Proteomic alterations of HDL fractions and their association with glycemic control was examined. Study population: 26 patients with T1DM and 13 HC. RESULTS/ANTICIPATED RESULTS: We quantified 78 proteins in isolated HDL, using mass spectrometry and label-free SWATH quantification. Youth with T1DM had significantly higher protein levels of A1BG (P = 0.008), A2AP (P = 0.0448), APOA4 (P = 0.0366), CFAH (P = 0.0476), FHR2 (P = 0.0005), ITIH4(P = 0.01), PGRP2 (P = 0.0167) and lower levels of ALBU (P = 0.0164) and CO3 (P = 0.019) compared to HC. A1BG (r=0.541, P<0.001) and ITIH4 (r=0.357, P = 0.026) were significantly positively correlated with HbA1c. DISCUSSION/SIGNIFICANCE OF IMPACT: Youth with T1DM have proteomic alterations of their HDL compared to HC, despite similar concentration of HDL cholesterol, that might affect the cardioprotective mechanisms of HDL. Future efforts should focus on investigating the role of these HDL associated proteins in regard to HDL function and their role in CVD risk in patients with T1DM. |
| first_indexed | 2024-04-10T04:54:32Z |
| format | Article |
| id | doaj.art-38b5a0a2dd8741cdacc7ae656072e279 |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-10T04:54:32Z |
| publishDate | 2019-03-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-38b5a0a2dd8741cdacc7ae656072e2792023-03-09T12:30:29ZengCambridge University PressJournal of Clinical and Translational Science2059-86612019-03-013313210.1017/cts.2019.763065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic controlEvgenia Gourgari0Scott Gordon1Junfeng Ma2Martin Playford3Nehal Mehta4Radoslav Goldman5Alan Remaley6Georgetown - Howard UniversitiesGeorgetown - Howard UniversitiesGeorgetown - Howard UniversitiesGeorgetown - Howard UniversitiesGeorgetown - Howard UniversitiesGeorgetown - Howard UniversitiesGeorgetown - Howard UniversitiesOBJECTIVES/SPECIFIC AIMS: Our objective was to compare the proteomics of HDL between youth with T1DM and healthy controls (HC). METHODS/STUDY POPULATION: We did chromatography-based HDL purification and SWATH-MS-based proteomic quantitation. Proteomic alterations of HDL fractions and their association with glycemic control was examined. Study population: 26 patients with T1DM and 13 HC. RESULTS/ANTICIPATED RESULTS: We quantified 78 proteins in isolated HDL, using mass spectrometry and label-free SWATH quantification. Youth with T1DM had significantly higher protein levels of A1BG (P = 0.008), A2AP (P = 0.0448), APOA4 (P = 0.0366), CFAH (P = 0.0476), FHR2 (P = 0.0005), ITIH4(P = 0.01), PGRP2 (P = 0.0167) and lower levels of ALBU (P = 0.0164) and CO3 (P = 0.019) compared to HC. A1BG (r=0.541, P<0.001) and ITIH4 (r=0.357, P = 0.026) were significantly positively correlated with HbA1c. DISCUSSION/SIGNIFICANCE OF IMPACT: Youth with T1DM have proteomic alterations of their HDL compared to HC, despite similar concentration of HDL cholesterol, that might affect the cardioprotective mechanisms of HDL. Future efforts should focus on investigating the role of these HDL associated proteins in regard to HDL function and their role in CVD risk in patients with T1DM.https://www.cambridge.org/core/product/identifier/S2059866119000761/type/journal_article |
| spellingShingle | Evgenia Gourgari Scott Gordon Junfeng Ma Martin Playford Nehal Mehta Radoslav Goldman Alan Remaley 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control Journal of Clinical and Translational Science |
| title | 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control |
| title_full | 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control |
| title_fullStr | 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control |
| title_full_unstemmed | 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control |
| title_short | 3065 A1BG and ITIH4 proteins are upregulated on HDL of youth with type 1 diabetes and correlate with glycemic control |
| title_sort | 3065 a1bg and itih4 proteins are upregulated on hdl of youth with type 1 diabetes and correlate with glycemic control |
| url | https://www.cambridge.org/core/product/identifier/S2059866119000761/type/journal_article |
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