Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR
Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagul...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2017-02-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201702-0004_characterisation-of-methicillin-susceptible-staphylococcus-pseudintermedius-isolates-from-canine-infections-and.php |
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| author | O. Melter P. Svec J. Tkadlec J. Doskar H. Kinska R. Pantucek |
| author_facet | O. Melter P. Svec J. Tkadlec J. Doskar H. Kinska R. Pantucek |
| author_sort | O. Melter |
| collection | DOAJ |
| description | Staphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (seccanine) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin. |
| first_indexed | 2024-04-10T07:58:22Z |
| format | Article |
| id | doaj.art-38b674c0a1ae4ff3b31e58f018bdb883 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:22Z |
| publishDate | 2017-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-38b674c0a1ae4ff3b31e58f018bdb8832023-02-23T03:50:40ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922017-02-01622818910.17221/105/2016-VETMEDvet-201702-0004Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCRO. Melter0P. Svec1J. Tkadlec2J. Doskar3H. Kinska4R. Pantucek52nd Medical Faculty, Charles University, Prague, Czech RepublicFaculty of Science, Masaryk University, Brno, Czech Republic2nd Medical Faculty, Charles University, Prague, Czech RepublicFaculty of Science, Masaryk University, Brno, Czech RepublicLaboratory of Veterinary Medicine (Labvet), Prague, Czech RepublicFaculty of Science, Masaryk University, Brno, Czech RepublicStaphylococcus pseudintermedius is a genuine opportunistic pathogen of the skin, especially in canids. However, characterisation of virulence, antimicrobial resistance and genotypic variability in methicillin-susceptible S. pseudintermedius isolates has not been fully explored. In this study, coagulase-positive staphylococcal isolates collected from dogs of various breeds and ages suffering from dermatitis (n = 70), pyoderma (n = 7), and otitis (n = 7), from districts of Prague (Czech Republic) and surrounding areas, were characterised using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry, and repetitive sequence-based PCR fingerprinting. Susceptibility to antimicrobial agents was determined, virulence factor genes for leukocidin (lukSF-I), exfoliatins (exi, expB, and siet), enterotoxin C (seccanine) and enterotoxin-related genes (se-int and sel) were detected using multiplex PCR and the genotypes of S. pseudintermedius isolates were determined using SmaI macrorestriction analysis. The majority of the staphylococcal isolates (n = 84) were identified as S. pseudintermedius (n = 79) and all of them were susceptible to methicillin/oxacillin (MSSP). About half of the strains (n = 41) were resistant to macrolide-lincosamide-streptogramin B antimicrobial agents and resistance was mediated in all but one of the strains by the erm(B) gene. The genes for lukSF-I, siet, se-int, and sel were detected in the majority of the MSSP strains (96.2%, 100%, 100%, and 73.4%, respectively). Investigated canine S. pseudintermedius isolates were highly heterogeneous, which prevented the correlation of any specific lineage to a particular infection, dog breed, or region of origin.https://vetmed.agriculturejournals.cz/artkey/vet-201702-0004_characterisation-of-methicillin-susceptible-staphylococcus-pseudintermedius-isolates-from-canine-infections-and.phpstaphylococcus pseudintermediusmacrolide lincosamide-streptogramin b (mlsb) resistancegenotypingvirulence genes |
| spellingShingle | O. Melter P. Svec J. Tkadlec J. Doskar H. Kinska R. Pantucek Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR Veterinární Medicína staphylococcus pseudintermedius macrolide lincosamide-streptogramin b (mlsb) resistance genotyping virulence genes |
| title | Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR |
| title_full | Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR |
| title_fullStr | Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR |
| title_full_unstemmed | Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR |
| title_short | Characterisation of methicillin-susceptible Staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex PCR |
| title_sort | characterisation of methicillin susceptible staphylococcus pseudintermedius isolates from canine infections and determination of virulence factors using multiplex pcr |
| topic | staphylococcus pseudintermedius macrolide lincosamide-streptogramin b (mlsb) resistance genotyping virulence genes |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201702-0004_characterisation-of-methicillin-susceptible-staphylococcus-pseudintermedius-isolates-from-canine-infections-and.php |
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