Effects of substrate stiffness on mast cell migration
Mast cells (MCs) play important roles in multiple pathologies, including fibrosis; however, their behaviors in different extracellular matrix (ECM) environments have not been fully elucidated. Accordingly, in this study, the migration of MCs on substrates with different stiffnesses was investigated...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933521000297 |
| _version_ | 1818342619301806080 |
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| author | Yi Yu Liu-Jie Ren Xin-Yue Liu Xiao-Bo Gong Wei Yao |
| author_facet | Yi Yu Liu-Jie Ren Xin-Yue Liu Xiao-Bo Gong Wei Yao |
| author_sort | Yi Yu |
| collection | DOAJ |
| description | Mast cells (MCs) play important roles in multiple pathologies, including fibrosis; however, their behaviors in different extracellular matrix (ECM) environments have not been fully elucidated. Accordingly, in this study, the migration of MCs on substrates with different stiffnesses was investigated using time-lapse video microscopy. Our results showed that MCs could appear in round, spindle, and star-like shapes; spindle-shaped cells accounted for 80–90 % of the total observed cells. The migration speed of round cells was significantly lower than that of cells with other shapes. Interestingly, spindle-shaped MCs migrated in a jiggling and wiggling motion between protrusions. The persistence index of MC migration was slightly higher on stiffer substrates. Moreover, we found that there was an intermediate optimal stiffness at which the migration efficiency was the highest. These findings may help to improve our understanding of MC-induced pathologies and the roles of MC migration in the immune system. |
| first_indexed | 2024-12-13T16:17:34Z |
| format | Article |
| id | doaj.art-38bac367924d42499ff73b25696a9968 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-12-13T16:17:34Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-38bac367924d42499ff73b25696a99682022-12-21T23:38:49ZengElsevierEuropean Journal of Cell Biology0171-93352021-09-011007151178Effects of substrate stiffness on mast cell migrationYi Yu0Liu-Jie Ren1Xin-Yue Liu2Xiao-Bo Gong3Wei Yao4Shanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Department of Aeronautics and Astronautics, Fudan University, No.220 Handan Road, Shanghai, 200433, ChinaENT Institute, Eye & ENT Hospital of Fudan University, No.83 Fenyang Road, Shanghai, 200031, ChinaShanghai Institute of Applied Mathematics and Mechanics, School of Mechanics and Engineering Science, Shanghai Key Laboratory of Mechanics in Energy Engineering, Shanghai University, Shanghai 200072, ChinaKey Laboratory of Hydrodynamics (Ministry of Education), Department of Engineering Mechanics, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaShanghai Key Laboratory of Acupuncture Mechanism and Acupoint Function, Department of Aeronautics and Astronautics, Fudan University, No.220 Handan Road, Shanghai, 200433, China; Corresponding author.Mast cells (MCs) play important roles in multiple pathologies, including fibrosis; however, their behaviors in different extracellular matrix (ECM) environments have not been fully elucidated. Accordingly, in this study, the migration of MCs on substrates with different stiffnesses was investigated using time-lapse video microscopy. Our results showed that MCs could appear in round, spindle, and star-like shapes; spindle-shaped cells accounted for 80–90 % of the total observed cells. The migration speed of round cells was significantly lower than that of cells with other shapes. Interestingly, spindle-shaped MCs migrated in a jiggling and wiggling motion between protrusions. The persistence index of MC migration was slightly higher on stiffer substrates. Moreover, we found that there was an intermediate optimal stiffness at which the migration efficiency was the highest. These findings may help to improve our understanding of MC-induced pathologies and the roles of MC migration in the immune system.http://www.sciencedirect.com/science/article/pii/S0171933521000297Mast cellsMigrationStiffnessTrajectoryCell morphology |
| spellingShingle | Yi Yu Liu-Jie Ren Xin-Yue Liu Xiao-Bo Gong Wei Yao Effects of substrate stiffness on mast cell migration European Journal of Cell Biology Mast cells Migration Stiffness Trajectory Cell morphology |
| title | Effects of substrate stiffness on mast cell migration |
| title_full | Effects of substrate stiffness on mast cell migration |
| title_fullStr | Effects of substrate stiffness on mast cell migration |
| title_full_unstemmed | Effects of substrate stiffness on mast cell migration |
| title_short | Effects of substrate stiffness on mast cell migration |
| title_sort | effects of substrate stiffness on mast cell migration |
| topic | Mast cells Migration Stiffness Trajectory Cell morphology |
| url | http://www.sciencedirect.com/science/article/pii/S0171933521000297 |
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