Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia
Cytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) gene is one of the sub-members of CYP450 family member and it encodes with the families of drug metabolizing enzyme families along with the cancers and leukemias. Among leukemias, AML is considered to be one of the important leukemia which attac...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Saudi Journal of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319562X23003625 |
| _version_ | 1797315972863885312 |
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| author | Abdullah Farasani |
| author_facet | Abdullah Farasani |
| author_sort | Abdullah Farasani |
| collection | DOAJ |
| description | Cytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) gene is one of the sub-members of CYP450 family member and it encodes with the families of drug metabolizing enzyme families along with the cancers and leukemias. Among leukemias, AML is considered to be one of the important leukemia which attack the older adults. The aim of this study is to explore the role of A4889G polymorphism in CYP1A1 gene in acute myeloid leukemia (AML) in the Saudi population. This study was designed as an experimental case-control study in which 100 AML cases and 100 controls were selected. This in vivo study was carried out using genomic DNA extraction, polymerase chain reaction and agarose gel electrophoresis and then BsrDI restriction enzyme to digest the A4889G polymorphism of the PCR products. In this study, 200 subjects were digested and based on the appearance of the bands, genotypes were categorized. The attained data was used to calculate the clinical details as well as genotype analysis. The study results confirmed AG genotype (OR = 3.23, CI = 1.60–6.55, p = 0.0008), AG + GG (OR = 3.47, CI = 1.76–6.86, p = 0.0002) and GG + AA (OR = 12.47, CI = 6.18–15.17, p < 0.0001) and G vs A (OR = 3.15, CI = 1.71–5.81, p = 0.0001) were associated in AML cases. In conclusion, we confirm that A4889G polymorphism is associated with AML in the Saudi population. |
| first_indexed | 2024-03-08T03:12:34Z |
| format | Article |
| id | doaj.art-38bae9039a314a378490fc65f0f8822c |
| institution | Directory Open Access Journal |
| issn | 1319-562X |
| language | English |
| last_indexed | 2024-03-08T03:12:34Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Journal of Biological Sciences |
| spelling | doaj.art-38bae9039a314a378490fc65f0f8822c2024-02-13T04:06:23ZengElsevierSaudi Journal of Biological Sciences1319-562X2024-03-01313103917Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemiaAbdullah Farasani0Biomedical Research Unit, Medical Center, Jazan University, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi ArabiaCytochrome P450 Family 1 Subfamily A Member 1 (CYP1A1) gene is one of the sub-members of CYP450 family member and it encodes with the families of drug metabolizing enzyme families along with the cancers and leukemias. Among leukemias, AML is considered to be one of the important leukemia which attack the older adults. The aim of this study is to explore the role of A4889G polymorphism in CYP1A1 gene in acute myeloid leukemia (AML) in the Saudi population. This study was designed as an experimental case-control study in which 100 AML cases and 100 controls were selected. This in vivo study was carried out using genomic DNA extraction, polymerase chain reaction and agarose gel electrophoresis and then BsrDI restriction enzyme to digest the A4889G polymorphism of the PCR products. In this study, 200 subjects were digested and based on the appearance of the bands, genotypes were categorized. The attained data was used to calculate the clinical details as well as genotype analysis. The study results confirmed AG genotype (OR = 3.23, CI = 1.60–6.55, p = 0.0008), AG + GG (OR = 3.47, CI = 1.76–6.86, p = 0.0002) and GG + AA (OR = 12.47, CI = 6.18–15.17, p < 0.0001) and G vs A (OR = 3.15, CI = 1.71–5.81, p = 0.0001) were associated in AML cases. In conclusion, we confirm that A4889G polymorphism is associated with AML in the Saudi population.http://www.sciencedirect.com/science/article/pii/S1319562X23003625AMLA4889G polymorphismCYP1A1 gene and Saudi Arabian adults |
| spellingShingle | Abdullah Farasani Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia Saudi Journal of Biological Sciences AML A4889G polymorphism CYP1A1 gene and Saudi Arabian adults |
| title | Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia |
| title_full | Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia |
| title_fullStr | Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia |
| title_full_unstemmed | Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia |
| title_short | Association of genetic predisposition studies in CYP1A1 polymorphism studies in acute myeloid leukemia |
| title_sort | association of genetic predisposition studies in cyp1a1 polymorphism studies in acute myeloid leukemia |
| topic | AML A4889G polymorphism CYP1A1 gene and Saudi Arabian adults |
| url | http://www.sciencedirect.com/science/article/pii/S1319562X23003625 |
| work_keys_str_mv | AT abdullahfarasani associationofgeneticpredispositionstudiesincyp1a1polymorphismstudiesinacutemyeloidleukemia |