LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma
Abstract Background Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is overexpressed in multiple human tumors. However, the role of LPCAT1 in hepatocellular carcinoma (HCC) has not been understood. We aim to explore the relationships between LPCAT1 expression and prognosis, clinicopathological fe...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-10-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-022-00854-1 |
| _version_ | 1811197885682810880 |
|---|---|
| author | Lan Li Xiao Wang Yanni Ding Nini Hui Bingjie Su Min Yang |
| author_facet | Lan Li Xiao Wang Yanni Ding Nini Hui Bingjie Su Min Yang |
| author_sort | Lan Li |
| collection | DOAJ |
| description | Abstract Background Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is overexpressed in multiple human tumors. However, the role of LPCAT1 in hepatocellular carcinoma (HCC) has not been understood. We aim to explore the relationships between LPCAT1 expression and prognosis, clinicopathological features, tumor microenvironment (TME), immune cell infiltration, immune checkpoint gene expression, and related signaling pathways in HCC. Furthermore, we also explored the relationship between LPCAT1 expression and drug sensitivity to HCC treatment. Methods The expression profiles were acquired from the Cancer Genome Atlas (TCGA) and the Human Protein Atlas (THPA). Immune status and infiltration in cancer tissues were explored using the single sample gene set enrichment analysis (ssGSEA) and CIBERSORT algorithm. Results LPCAT1 was overexpressed in HCC, and its expression was related to poor prognosis, LPCAT1 was an independent prognostic biomarker in HCC. Expression of LPCAT1 increased statistically with the increase of clinical stage and grade of HCC patients. GO and KEGG network analysis revealed that LPCAT1 positively associated molecules were mostly enriched in functions related to cell adhesion. The TME score of high-LPCAT1 group was significantly higher than that of low-LPCAT1 group. Immune infiltrating cells positively correlated with LPCAT1 expression were Macrophages M0, B cells memory, Dendritic cells activated, T cells regulatory and T cells gamma delta in HCC. We found a positive correlation between LPCAT1 and most immune checkpoint gene expression. The IC50 of 5-Fluorouracil, Gemcitabine, Mitomycin C, Sorafenib and Cabozantinib in patients with high-LPCAT1 expression was lower than that in patients with low-LPCAT1 expression. Our findings provide a wealth of information for further understanding of the biological functions and signaling pathways of LPCAT1 in HCC. Conclusions LPCAT1 is an independent prognostic biomarker and associated with tumor microenvironment, immune cell infiltration, immune checkpoint expression and drug sensitivity in hepatocellular carcinoma. |
| first_indexed | 2024-04-12T01:20:45Z |
| format | Article |
| id | doaj.art-38be17f22535413d8cea5b0454b88acc |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-12T01:20:45Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-38be17f22535413d8cea5b0454b88acc2022-12-22T03:53:47ZengBMCEuropean Journal of Medical Research2047-783X2022-10-0127111210.1186/s40001-022-00854-1LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinomaLan Li0Xiao Wang1Yanni Ding2Nini Hui3Bingjie Su4Min Yang5Department of Breast Surgery, Shaanxi Provincial Cancer HospitalDepartment of Breast Surgery, Shaanxi Provincial Cancer HospitalDepartment of Breast Surgery, Shaanxi Provincial Cancer HospitalDepartment of Breast Surgery, Shaanxi Provincial Cancer HospitalDepartment of Breast Surgery, Shaanxi Provincial Cancer HospitalDepartment of Breast Surgery, Shaanxi Provincial Cancer HospitalAbstract Background Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is overexpressed in multiple human tumors. However, the role of LPCAT1 in hepatocellular carcinoma (HCC) has not been understood. We aim to explore the relationships between LPCAT1 expression and prognosis, clinicopathological features, tumor microenvironment (TME), immune cell infiltration, immune checkpoint gene expression, and related signaling pathways in HCC. Furthermore, we also explored the relationship between LPCAT1 expression and drug sensitivity to HCC treatment. Methods The expression profiles were acquired from the Cancer Genome Atlas (TCGA) and the Human Protein Atlas (THPA). Immune status and infiltration in cancer tissues were explored using the single sample gene set enrichment analysis (ssGSEA) and CIBERSORT algorithm. Results LPCAT1 was overexpressed in HCC, and its expression was related to poor prognosis, LPCAT1 was an independent prognostic biomarker in HCC. Expression of LPCAT1 increased statistically with the increase of clinical stage and grade of HCC patients. GO and KEGG network analysis revealed that LPCAT1 positively associated molecules were mostly enriched in functions related to cell adhesion. The TME score of high-LPCAT1 group was significantly higher than that of low-LPCAT1 group. Immune infiltrating cells positively correlated with LPCAT1 expression were Macrophages M0, B cells memory, Dendritic cells activated, T cells regulatory and T cells gamma delta in HCC. We found a positive correlation between LPCAT1 and most immune checkpoint gene expression. The IC50 of 5-Fluorouracil, Gemcitabine, Mitomycin C, Sorafenib and Cabozantinib in patients with high-LPCAT1 expression was lower than that in patients with low-LPCAT1 expression. Our findings provide a wealth of information for further understanding of the biological functions and signaling pathways of LPCAT1 in HCC. Conclusions LPCAT1 is an independent prognostic biomarker and associated with tumor microenvironment, immune cell infiltration, immune checkpoint expression and drug sensitivity in hepatocellular carcinoma.https://doi.org/10.1186/s40001-022-00854-1LPCAT1PrognosisTumor microenvironmentImmune cell infiltrationImmune checkpointDrug sensitivity |
| spellingShingle | Lan Li Xiao Wang Yanni Ding Nini Hui Bingjie Su Min Yang LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma European Journal of Medical Research LPCAT1 Prognosis Tumor microenvironment Immune cell infiltration Immune checkpoint Drug sensitivity |
| title | LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| title_full | LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| title_fullStr | LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| title_full_unstemmed | LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| title_short | LPCAT1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| title_sort | lpcat1 acts as an independent prognostic biomarker correlated with immune infiltration in hepatocellular carcinoma |
| topic | LPCAT1 Prognosis Tumor microenvironment Immune cell infiltration Immune checkpoint Drug sensitivity |
| url | https://doi.org/10.1186/s40001-022-00854-1 |
| work_keys_str_mv | AT lanli lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma AT xiaowang lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma AT yanniding lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma AT ninihui lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma AT bingjiesu lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma AT minyang lpcat1actsasanindependentprognosticbiomarkercorrelatedwithimmuneinfiltrationinhepatocellularcarcinoma |