Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks
Fluorescence analysis of materials used as binders and coatings in artworks is often hampered by numerous factors, leading to uncertainties in data interpretation. Fluorescence lifetime (FL) measurements enable improvement of the specificity with respect to steady-state measurements by resolving the...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/1/179 |
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| author | Alice Dal Fovo Sara Mattana Antonina Chaban Diego Quintero Balbas João Luis Lagarto Jana Striova Riccardo Cicchi Raffaella Fontana |
| author_facet | Alice Dal Fovo Sara Mattana Antonina Chaban Diego Quintero Balbas João Luis Lagarto Jana Striova Riccardo Cicchi Raffaella Fontana |
| author_sort | Alice Dal Fovo |
| collection | DOAJ |
| description | Fluorescence analysis of materials used as binders and coatings in artworks is often hampered by numerous factors, leading to uncertainties in data interpretation. Fluorescence lifetime (FL) measurements enable improvement of the specificity with respect to steady-state measurements by resolving the decay dynamics of the fluorophore emissions. In this work, layers of natural resin, oil, and wax—in pure form, pigmented, in mixtures, and spread on different substrates—were analyzed using a compact, portable, fiber-based FL instrument. FL data were processed via the phasor method and integrated with Raman spectroscopy to obtain complementary chemical information on the different substances. It was observed that the <i>τ</i>-phase of the mixtures is affected by both the pigments and the dispersing medium, and that the presence of the metal substrate contributes to changes in the FL of mixtures. The results obtained with our portable FL system combined with Raman spectroscopy pave the way for a systematic study of a larger number of materials for future in situ applications on works of art. |
| first_indexed | 2024-03-10T03:50:05Z |
| format | Article |
| id | doaj.art-38be9f3df4b249de9b8120139bc8820a |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:50:05Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-38be9f3df4b249de9b8120139bc8820a2023-11-23T11:08:56ZengMDPI AGApplied Sciences2076-34172021-12-0112117910.3390/app12010179Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in ArtworksAlice Dal Fovo0Sara Mattana1Antonina Chaban2Diego Quintero Balbas3João Luis Lagarto4Jana Striova5Riccardo Cicchi6Raffaella Fontana7National Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyNational Research Council—National Institute of Optics (CNR-INO), Largo E. Fermi 6, 50125 Florence, ItalyFluorescence analysis of materials used as binders and coatings in artworks is often hampered by numerous factors, leading to uncertainties in data interpretation. Fluorescence lifetime (FL) measurements enable improvement of the specificity with respect to steady-state measurements by resolving the decay dynamics of the fluorophore emissions. In this work, layers of natural resin, oil, and wax—in pure form, pigmented, in mixtures, and spread on different substrates—were analyzed using a compact, portable, fiber-based FL instrument. FL data were processed via the phasor method and integrated with Raman spectroscopy to obtain complementary chemical information on the different substances. It was observed that the <i>τ</i>-phase of the mixtures is affected by both the pigments and the dispersing medium, and that the presence of the metal substrate contributes to changes in the FL of mixtures. The results obtained with our portable FL system combined with Raman spectroscopy pave the way for a systematic study of a larger number of materials for future in situ applications on works of art.https://www.mdpi.com/2076-3417/12/1/179fluorescence lifetimeRaman spectroscopyphasor analysisorganic bindersfluorescent pigments |
| spellingShingle | Alice Dal Fovo Sara Mattana Antonina Chaban Diego Quintero Balbas João Luis Lagarto Jana Striova Riccardo Cicchi Raffaella Fontana Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks Applied Sciences fluorescence lifetime Raman spectroscopy phasor analysis organic binders fluorescent pigments |
| title | Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks |
| title_full | Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks |
| title_fullStr | Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks |
| title_full_unstemmed | Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks |
| title_short | Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks |
| title_sort | fluorescence lifetime phasor analysis and raman spectroscopy of pigmented organic binders and coatings used in artworks |
| topic | fluorescence lifetime Raman spectroscopy phasor analysis organic binders fluorescent pigments |
| url | https://www.mdpi.com/2076-3417/12/1/179 |
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