Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding
AbstractPaclitaxel (trademark Taxol®) is the most effective chemotherapeutic agent against a wide range of cancers. Hazelnut (Corylus avellana L.) cell culture is a promising and inexpensive strategy for producing paclitaxel and related taxanes. This study evaluated the effect of elicitation and tax...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2255703 |
| _version_ | 1797367459697655808 |
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| author | Ilayda Goktepe-Atilgan Ahmet Dogan Sule Ari |
| author_facet | Ilayda Goktepe-Atilgan Ahmet Dogan Sule Ari |
| author_sort | Ilayda Goktepe-Atilgan |
| collection | DOAJ |
| description | AbstractPaclitaxel (trademark Taxol®) is the most effective chemotherapeutic agent against a wide range of cancers. Hazelnut (Corylus avellana L.) cell culture is a promising and inexpensive strategy for producing paclitaxel and related taxanes. This study evaluated the effect of elicitation and taxane precursor feeding for the enhancement of taxanes in a Turkish hazelnut (C. avellana cv. ‘Kalınkara’) cell suspension culture. Elicitor methyl jasmonate (100, 200 and 300 µmol L−1) and precursor phenylalanine (3μmol, 3 and 6 mmol L−1) were tested in cell suspension cultures. While methyl jasmonate increased the accumulation of taxanes, especially cephalomannine, via induction of the paclitaxel biosynthetic pathway, phenylalanine slightly induced the conversion of 10-deacetylbaccatin III and baccatin III to the end product paclitaxel (0.5349 µg g−1). The results showed that 200 µmol L−1 methyl jasmonate led to significantly greater induction of taxanes than 100 and 300 µmol L−1. The amount of cephalomannine (331.6 µg g−1) in cell suspension cultures elicited by 200 µmol L−1 methyl jasmonate was 8-fold higher than that in the control (41.61 µg g−1), while 10-deacetylbaccatin III (6.174 µg g−1) and baccatin III (3.956 µg g−1) increased almost twice as much as the control. The highest total paclitaxel yield was 5.453 µg g−1 in the same culture conditions. In conclusion, cell culture systems of C. avellana L. cv. ‘Kalınkara’ hazelnut, established for the first time, may become a potential candidate for industrial-level production of cephalomannine and other taxanes with further optimization. |
| first_indexed | 2024-03-08T17:18:39Z |
| format | Article |
| id | doaj.art-38c21174b5fe43e4b92e7887c1b6fd7c |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-03-08T17:18:39Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-38c21174b5fe43e4b92e7887c1b6fd7c2024-01-03T09:44:11ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302023-12-0137110.1080/13102818.2023.2255703Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feedingIlayda Goktepe-Atilgan0Ahmet Dogan1Sule Ari2Faculty of Science, Department of Molecular Biology and Genetics, İstanbul University, Istanbul, TurkeyFaculty of Science, Department of Molecular Biology and Genetics, İstanbul University, Istanbul, TurkeyFaculty of Science, Department of Molecular Biology and Genetics, İstanbul University, Istanbul, TurkeyAbstractPaclitaxel (trademark Taxol®) is the most effective chemotherapeutic agent against a wide range of cancers. Hazelnut (Corylus avellana L.) cell culture is a promising and inexpensive strategy for producing paclitaxel and related taxanes. This study evaluated the effect of elicitation and taxane precursor feeding for the enhancement of taxanes in a Turkish hazelnut (C. avellana cv. ‘Kalınkara’) cell suspension culture. Elicitor methyl jasmonate (100, 200 and 300 µmol L−1) and precursor phenylalanine (3μmol, 3 and 6 mmol L−1) were tested in cell suspension cultures. While methyl jasmonate increased the accumulation of taxanes, especially cephalomannine, via induction of the paclitaxel biosynthetic pathway, phenylalanine slightly induced the conversion of 10-deacetylbaccatin III and baccatin III to the end product paclitaxel (0.5349 µg g−1). The results showed that 200 µmol L−1 methyl jasmonate led to significantly greater induction of taxanes than 100 and 300 µmol L−1. The amount of cephalomannine (331.6 µg g−1) in cell suspension cultures elicited by 200 µmol L−1 methyl jasmonate was 8-fold higher than that in the control (41.61 µg g−1), while 10-deacetylbaccatin III (6.174 µg g−1) and baccatin III (3.956 µg g−1) increased almost twice as much as the control. The highest total paclitaxel yield was 5.453 µg g−1 in the same culture conditions. In conclusion, cell culture systems of C. avellana L. cv. ‘Kalınkara’ hazelnut, established for the first time, may become a potential candidate for industrial-level production of cephalomannine and other taxanes with further optimization.https://www.tandfonline.com/doi/10.1080/13102818.2023.2255703Hazelnutanti-cancertaxanescell suspension culturemethyl jasmonatephenylalanine |
| spellingShingle | Ilayda Goktepe-Atilgan Ahmet Dogan Sule Ari Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding Biotechnology & Biotechnological Equipment Hazelnut anti-cancer taxanes cell suspension culture methyl jasmonate phenylalanine |
| title | Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding |
| title_full | Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding |
| title_fullStr | Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding |
| title_full_unstemmed | Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding |
| title_short | Enhancement of taxane production in cell suspension culture of Corylus avellana L. Kalınkara by elicitation and precursor feeding |
| title_sort | enhancement of taxane production in cell suspension culture of corylus avellana l kalinkara by elicitation and precursor feeding |
| topic | Hazelnut anti-cancer taxanes cell suspension culture methyl jasmonate phenylalanine |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2023.2255703 |
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