Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats
Abstract Recent pre-clinical and clinical spinal cord epidural stimulation (scES) experiments specifically targeting the thoracolumbar and lumbosacral circuitries mediating lower urinary tract (LUT) function have shown improvements in storage, detrusor pressure, and emptying. With the existence of a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39388-9 |
| _version_ | 1797769559418077184 |
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| author | Daniel Medina-Aguiñaga Robert F. Hoey Natasha L. Wilkins Beatrice Ugiliweneza Jason Fell Susan J. Harkema Charles H. Hubscher |
| author_facet | Daniel Medina-Aguiñaga Robert F. Hoey Natasha L. Wilkins Beatrice Ugiliweneza Jason Fell Susan J. Harkema Charles H. Hubscher |
| author_sort | Daniel Medina-Aguiñaga |
| collection | DOAJ |
| description | Abstract Recent pre-clinical and clinical spinal cord epidural stimulation (scES) experiments specifically targeting the thoracolumbar and lumbosacral circuitries mediating lower urinary tract (LUT) function have shown improvements in storage, detrusor pressure, and emptying. With the existence of a lumbar spinal coordinating center in rats that is involved with external urethral sphincter (EUS) functionality during micturition, the mid-lumbar spinal cord (specifically L3) was targeted in the current study with scES to determine if the EUS and thus the void pattern could be modulated, using both intact and chronic complete spinal cord injured female rats under urethane anesthesia. L3 scES at select frequencies and intensities of stimulation produced a reduction in void volumes and EUS burst duration in intact rats. After chronic transection, three different subgroups of LUT dysfunction were identified and the response to L3 scES promoted different cystometry outcomes, including changes in EUS bursting. The current findings suggest that scES at the L3 level can generate functional neuromodulation of both the urinary bladder and the EUS in intact and SCI rats to enhance voiding in a variety of clinical scenarios. |
| first_indexed | 2024-03-12T21:10:49Z |
| format | Article |
| id | doaj.art-38c35f99e0944758b6bb327bd112e2d9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T21:10:49Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-38c35f99e0944758b6bb327bd112e2d92023-07-30T11:11:46ZengNature PortfolioScientific Reports2045-23222023-07-0113111410.1038/s41598-023-39388-9Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized ratsDaniel Medina-Aguiñaga0Robert F. Hoey1Natasha L. Wilkins2Beatrice Ugiliweneza3Jason Fell4Susan J. Harkema5Charles H. Hubscher6Department of Anatomical Sciences and Neurobiology, University of Louisville School of MedicineDepartment of Anatomical Sciences and Neurobiology, University of Louisville School of MedicineDepartment of Anatomical Sciences and Neurobiology, University of Louisville School of MedicineDepartment of Neurological Surgery, University of Louisville School of MedicineDepartment of Anatomical Sciences and Neurobiology, University of Louisville School of MedicineDepartment of Neurological Surgery, University of Louisville School of MedicineDepartment of Anatomical Sciences and Neurobiology, University of Louisville School of MedicineAbstract Recent pre-clinical and clinical spinal cord epidural stimulation (scES) experiments specifically targeting the thoracolumbar and lumbosacral circuitries mediating lower urinary tract (LUT) function have shown improvements in storage, detrusor pressure, and emptying. With the existence of a lumbar spinal coordinating center in rats that is involved with external urethral sphincter (EUS) functionality during micturition, the mid-lumbar spinal cord (specifically L3) was targeted in the current study with scES to determine if the EUS and thus the void pattern could be modulated, using both intact and chronic complete spinal cord injured female rats under urethane anesthesia. L3 scES at select frequencies and intensities of stimulation produced a reduction in void volumes and EUS burst duration in intact rats. After chronic transection, three different subgroups of LUT dysfunction were identified and the response to L3 scES promoted different cystometry outcomes, including changes in EUS bursting. The current findings suggest that scES at the L3 level can generate functional neuromodulation of both the urinary bladder and the EUS in intact and SCI rats to enhance voiding in a variety of clinical scenarios.https://doi.org/10.1038/s41598-023-39388-9 |
| spellingShingle | Daniel Medina-Aguiñaga Robert F. Hoey Natasha L. Wilkins Beatrice Ugiliweneza Jason Fell Susan J. Harkema Charles H. Hubscher Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats Scientific Reports |
| title | Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats |
| title_full | Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats |
| title_fullStr | Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats |
| title_full_unstemmed | Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats |
| title_short | Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats |
| title_sort | mid lumbar l3 epidural stimulation effects on bladder and external urethral sphincter in non injured and chronically transected urethane anesthetized rats |
| url | https://doi.org/10.1038/s41598-023-39388-9 |
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