Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry
Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-l-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is impor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523005084 |
| _version_ | 1797244901630410752 |
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| author | Armaghan Shafaei Caroline R. Hill Jonathan M. Hodgson Lauren C. Blekkenhorst Mary C. Boyce |
| author_facet | Armaghan Shafaei Caroline R. Hill Jonathan M. Hodgson Lauren C. Blekkenhorst Mary C. Boyce |
| author_sort | Armaghan Shafaei |
| collection | DOAJ |
| description | Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-l-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more applicable to large studies. Quantitative analysis using calibration standards was demonstrated for 10 GLS, SFN and SMCSO. A further 10 GLS were tentatively identified using high resolution mass spectrometry. The use of surrogate GLS standards was shown to be unreliable, with closely related GLS displaying significantly different ionisation efficiencies. |
| first_indexed | 2024-03-08T22:31:09Z |
| format | Article |
| id | doaj.art-38c5e8fcf0284a8db83b438590ebfa93 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T19:18:22Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-38c5e8fcf0284a8db83b438590ebfa932024-03-26T04:27:04ZengElsevierFood Chemistry: X2590-15752024-03-0121101065Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometryArmaghan Shafaei0Caroline R. Hill1Jonathan M. Hodgson2Lauren C. Blekkenhorst3Mary C. Boyce4Centre for Integrative Metabolomics & Computational Biology, School of Science, Edith Cowan University, Joondalup, Western Australia, Australia; Corresponding author.Nutrition and Health Innovation Research Institute, School of Medical and Health Sciences, Edith Cowan University, Perth, Western Australia, AustraliaNutrition and Health Innovation Research Institute, School of Medical and Health Sciences, Edith Cowan University, Perth, Western Australia, Australia; Royal Perth Hospital Research Foundation, Perth, AustraliaNutrition and Health Innovation Research Institute, School of Medical and Health Sciences, Edith Cowan University, Perth, Western Australia, Australia; Royal Perth Hospital Research Foundation, Perth, AustraliaSchool of Science, Edith Cowan University, Joondalup, Western Australia, AustraliaSulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-l-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more applicable to large studies. Quantitative analysis using calibration standards was demonstrated for 10 GLS, SFN and SMCSO. A further 10 GLS were tentatively identified using high resolution mass spectrometry. The use of surrogate GLS standards was shown to be unreliable, with closely related GLS displaying significantly different ionisation efficiencies.http://www.sciencedirect.com/science/article/pii/S2590157523005084CruciferousBrassicaGlucosinolatesSulforaphane, S-methyl-l-cysteine sulfoxideLC-MS |
| spellingShingle | Armaghan Shafaei Caroline R. Hill Jonathan M. Hodgson Lauren C. Blekkenhorst Mary C. Boyce Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry Food Chemistry: X Cruciferous Brassica Glucosinolates Sulforaphane, S-methyl-l-cysteine sulfoxide LC-MS |
| title | Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| title_full | Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| title_fullStr | Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| title_full_unstemmed | Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| title_short | Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| title_sort | simultaneous extraction and quantitative analysis of s methyl l cysteine sulfoxide sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry |
| topic | Cruciferous Brassica Glucosinolates Sulforaphane, S-methyl-l-cysteine sulfoxide LC-MS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523005084 |
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