Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation
Bacillus subtilis PcrA abrogates replication-transcription conflicts in vivo and disrupts RecA nucleoprotein filaments in vitro. Inactivation of pcrA is lethal. We show that PcrA depletion lethality is suppressed by recJ (involved in end resection), recA (the recombinase), or mfd (transcription-coup...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmolb.2020.00140/full |
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| author | María Moreno-del Alamo Rubén Torres Candela Manfredi José A. Ruiz-Masó Gloria del Solar Juan Carlos Alonso |
| author_facet | María Moreno-del Alamo Rubén Torres Candela Manfredi José A. Ruiz-Masó Gloria del Solar Juan Carlos Alonso |
| author_sort | María Moreno-del Alamo |
| collection | DOAJ |
| description | Bacillus subtilis PcrA abrogates replication-transcription conflicts in vivo and disrupts RecA nucleoprotein filaments in vitro. Inactivation of pcrA is lethal. We show that PcrA depletion lethality is suppressed by recJ (involved in end resection), recA (the recombinase), or mfd (transcription-coupled repair) inactivation, but not by inactivating end resection (addAB or recQ), positive and negative RecA modulators (rarA or recX and recU), or genes involved in the reactivation of a stalled RNA polymerase (recD2, helD, hepA, and ywqA). We also report that B. subtilis mutations previously designated as recL16 actually map to the recO locus, and confirm that PcrA depletion lethality is suppressed by recO inactivation. The pcrA gene is epistatic to recA or mfd, but it is not epistatic to addAB, recJ, recQ, recO16, rarA, recX, recU, recD2, helD, hepA, or ywqA in response to DNA damage. PcrA depletion led to the accumulation of unsegregated chromosomes, and this defect is increased by recQ, rarA, or recU inactivation. We propose that PcrA, which is crucial to maintain cell viability, is involved in different DNA transactions. |
| first_indexed | 2024-04-13T18:45:07Z |
| format | Article |
| id | doaj.art-38c8eefaa2024ae5936baa4852cb3216 |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-13T18:45:07Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-38c8eefaa2024ae5936baa4852cb32162022-12-22T02:34:36ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2020-07-01710.3389/fmolb.2020.00140556588Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and SegregationMaría Moreno-del Alamo0Rubén Torres1Candela Manfredi2José A. Ruiz-Masó3Gloria del Solar4Juan Carlos Alonso5Department of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, SpainDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, SpainDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas, CIB-CSIC, Madrid, SpainCentro de Investigaciones Biológicas Margarita Salas, CIB-CSIC, Madrid, SpainDepartment of Microbial Biotechnology, Centro Nacional de Biotecnología, CNB-CSIC, Madrid, SpainBacillus subtilis PcrA abrogates replication-transcription conflicts in vivo and disrupts RecA nucleoprotein filaments in vitro. Inactivation of pcrA is lethal. We show that PcrA depletion lethality is suppressed by recJ (involved in end resection), recA (the recombinase), or mfd (transcription-coupled repair) inactivation, but not by inactivating end resection (addAB or recQ), positive and negative RecA modulators (rarA or recX and recU), or genes involved in the reactivation of a stalled RNA polymerase (recD2, helD, hepA, and ywqA). We also report that B. subtilis mutations previously designated as recL16 actually map to the recO locus, and confirm that PcrA depletion lethality is suppressed by recO inactivation. The pcrA gene is epistatic to recA or mfd, but it is not epistatic to addAB, recJ, recQ, recO16, rarA, recX, recU, recD2, helD, hepA, or ywqA in response to DNA damage. PcrA depletion led to the accumulation of unsegregated chromosomes, and this defect is increased by recQ, rarA, or recU inactivation. We propose that PcrA, which is crucial to maintain cell viability, is involved in different DNA transactions.https://www.frontiersin.org/article/10.3389/fmolb.2020.00140/fullreplication fork stallingRNA polymerase backtrackingreplication-transcription conflictRecL16RepUvrD |
| spellingShingle | María Moreno-del Alamo Rubén Torres Candela Manfredi José A. Ruiz-Masó Gloria del Solar Juan Carlos Alonso Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation Frontiers in Molecular Biosciences replication fork stalling RNA polymerase backtracking replication-transcription conflict RecL16 Rep UvrD |
| title | Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation |
| title_full | Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation |
| title_fullStr | Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation |
| title_full_unstemmed | Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation |
| title_short | Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation |
| title_sort | bacillus subtilis pcra couples dna replication transcription recombination and segregation |
| topic | replication fork stalling RNA polymerase backtracking replication-transcription conflict RecL16 Rep UvrD |
| url | https://www.frontiersin.org/article/10.3389/fmolb.2020.00140/full |
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