Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress
Bacterial ribosome small subunit rRNA (16S rRNA) contains 11 nucleotide modifications scattered throughout all its domains. The 16S rRNA pseudouridylation enzyme, RsuA, which modifies U516, is a survival protein essential for bacterial survival under stress conditions. A comparison of the growth cur...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1447 |
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| author | Sudeshi M. Abedeera Kumudie S. Jayalath Jiale Xie Rushdhi M. Rauff Sanjaya C. Abeysirigunawardena |
| author_facet | Sudeshi M. Abedeera Kumudie S. Jayalath Jiale Xie Rushdhi M. Rauff Sanjaya C. Abeysirigunawardena |
| author_sort | Sudeshi M. Abedeera |
| collection | DOAJ |
| description | Bacterial ribosome small subunit rRNA (16S rRNA) contains 11 nucleotide modifications scattered throughout all its domains. The 16S rRNA pseudouridylation enzyme, RsuA, which modifies U516, is a survival protein essential for bacterial survival under stress conditions. A comparison of the growth curves of wildtype and RsuA knock-out <i>E. coli</i> strains illustrates that RsuA renders a survival advantage to bacteria under streptomycin stress. The RsuA-dependent growth advantage for bacteria was found to be dependent on its pseudouridylation activity. In addition, the role of RsuA as a trans-acting factor during ribosome biogenesis may also play a role in bacterial growth under streptomycin stress. Furthermore, circular dichroism spectroscopy measurements and RNase footprinting studies have demonstrated that pseudouridine at position 516 influences helix 18 structure, folding, and streptomycin binding. This study exemplifies the importance of bacterial rRNA modification enzymes during environmental stress. |
| first_indexed | 2024-03-10T23:06:15Z |
| format | Article |
| id | doaj.art-38cb127d44f542c49d70c409a87a2e3e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:15Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-38cb127d44f542c49d70c409a87a2e3e2023-11-19T09:17:54ZengMDPI AGAntibiotics2079-63822023-09-01129144710.3390/antibiotics12091447Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin StressSudeshi M. Abedeera0Kumudie S. Jayalath1Jiale Xie2Rushdhi M. Rauff3Sanjaya C. Abeysirigunawardena4Department of Chemistry and Biochemistry, Kent State University, 1175 Risman Dr., Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, 1175 Risman Dr., Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, 1175 Risman Dr., Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, 1175 Risman Dr., Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, 1175 Risman Dr., Kent, OH 44242, USABacterial ribosome small subunit rRNA (16S rRNA) contains 11 nucleotide modifications scattered throughout all its domains. The 16S rRNA pseudouridylation enzyme, RsuA, which modifies U516, is a survival protein essential for bacterial survival under stress conditions. A comparison of the growth curves of wildtype and RsuA knock-out <i>E. coli</i> strains illustrates that RsuA renders a survival advantage to bacteria under streptomycin stress. The RsuA-dependent growth advantage for bacteria was found to be dependent on its pseudouridylation activity. In addition, the role of RsuA as a trans-acting factor during ribosome biogenesis may also play a role in bacterial growth under streptomycin stress. Furthermore, circular dichroism spectroscopy measurements and RNase footprinting studies have demonstrated that pseudouridine at position 516 influences helix 18 structure, folding, and streptomycin binding. This study exemplifies the importance of bacterial rRNA modification enzymes during environmental stress.https://www.mdpi.com/2079-6382/12/9/1447ribosomepseudouridinepseudouridine synthasestreptomycinhelix18 |
| spellingShingle | Sudeshi M. Abedeera Kumudie S. Jayalath Jiale Xie Rushdhi M. Rauff Sanjaya C. Abeysirigunawardena Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress Antibiotics ribosome pseudouridine pseudouridine synthase streptomycin helix18 |
| title | Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress |
| title_full | Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress |
| title_fullStr | Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress |
| title_full_unstemmed | Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress |
| title_short | Pseudouridine Synthase RsuA Confers a Survival Advantage to Bacteria under Streptomycin Stress |
| title_sort | pseudouridine synthase rsua confers a survival advantage to bacteria under streptomycin stress |
| topic | ribosome pseudouridine pseudouridine synthase streptomycin helix18 |
| url | https://www.mdpi.com/2079-6382/12/9/1447 |
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