Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages
Abstract Background Anthocyanin synthase (ANS) is the enzyme downstream of the anthocyanins synthesis pathway and the rate-limiting enzyme of the synthesis pathway. It catalyzes the conversion of colorless anthocyanins to anthocyanins and plays an important role in plant color presentation and stres...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04648-3 |
| _version_ | 1797398066050891776 |
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| author | Yongqing Feng Xuechun Tian Wei Liang XinTong Nan Aoning Zhang Wenfang Li Zonghuan Ma |
| author_facet | Yongqing Feng Xuechun Tian Wei Liang XinTong Nan Aoning Zhang Wenfang Li Zonghuan Ma |
| author_sort | Yongqing Feng |
| collection | DOAJ |
| description | Abstract Background Anthocyanin synthase (ANS) is the enzyme downstream of the anthocyanins synthesis pathway and the rate-limiting enzyme of the synthesis pathway. It catalyzes the conversion of colorless anthocyanins to anthocyanins and plays an important role in plant color presentation and stress resistance. However, ANS gene is rarely studied in grapes. Results In this study, 121 VvANS genes were identified and distributed on 18 chromosomes, VvANS family members were divided into 8 subgroups. Secondary structure prediction showed mainly irregular coils and α-helices, and subcellular localization indicated that VvANS gene family is mainly located in chloroplast, cytoplasm and nucleus. The promoter region of the VvANS gene family contains multiple cis-acting elements that are associated with light, abiotic stress, and hormones. Intraspecific collinearity analysis showed that there were 13 pairs of collinearity between VvANS genes. Interspecific collinearity analysis showed that there was more collinearity between grape, apple and Arabidopsis, but less collinearity between grape and rice. Microarray data analysis showed that VvANS17, VvANS23 and VvANS75 had higher expression levels in flesh and peel, while VvANS25, VvANS64 and VvANS106 had higher expression levels in flower. The results of qRT-PCR analysis showed that VvANS genes were expressed throughout the whole process of fruit coloring, such as VvANS47 and VvANS55 in the green fruit stage, VvANS3, VvANS64 and VvANS90 in the initial fruit color turning stage. The expression levels of VvANS21, VvANS79 and VvANS108 were higher at 50% coloring stage, indicating that these genes play an important role in the fruit coloring process. VvANS4, VvANS66 and VvANS113 had the highest expression levels in the full maturity stage. Conclusions These results indicated that different members of VvANS gene family played a role in different coloring stages, and this study laid a foundation for further research on the function of ANS gene family. |
| first_indexed | 2024-03-09T01:19:20Z |
| format | Article |
| id | doaj.art-38cb6c29276d4b9197ef9b45b1d3871c |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T01:19:20Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-38cb6c29276d4b9197ef9b45b1d3871c2023-12-10T12:13:28ZengBMCBMC Plant Biology1471-22292023-12-0123111710.1186/s12870-023-04648-3Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stagesYongqing Feng0Xuechun Tian1Wei Liang2XinTong Nan3Aoning Zhang4Wenfang Li5Zonghuan Ma6College of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityCollege of Horticulture, Gansu Agricultural UniversityAbstract Background Anthocyanin synthase (ANS) is the enzyme downstream of the anthocyanins synthesis pathway and the rate-limiting enzyme of the synthesis pathway. It catalyzes the conversion of colorless anthocyanins to anthocyanins and plays an important role in plant color presentation and stress resistance. However, ANS gene is rarely studied in grapes. Results In this study, 121 VvANS genes were identified and distributed on 18 chromosomes, VvANS family members were divided into 8 subgroups. Secondary structure prediction showed mainly irregular coils and α-helices, and subcellular localization indicated that VvANS gene family is mainly located in chloroplast, cytoplasm and nucleus. The promoter region of the VvANS gene family contains multiple cis-acting elements that are associated with light, abiotic stress, and hormones. Intraspecific collinearity analysis showed that there were 13 pairs of collinearity between VvANS genes. Interspecific collinearity analysis showed that there was more collinearity between grape, apple and Arabidopsis, but less collinearity between grape and rice. Microarray data analysis showed that VvANS17, VvANS23 and VvANS75 had higher expression levels in flesh and peel, while VvANS25, VvANS64 and VvANS106 had higher expression levels in flower. The results of qRT-PCR analysis showed that VvANS genes were expressed throughout the whole process of fruit coloring, such as VvANS47 and VvANS55 in the green fruit stage, VvANS3, VvANS64 and VvANS90 in the initial fruit color turning stage. The expression levels of VvANS21, VvANS79 and VvANS108 were higher at 50% coloring stage, indicating that these genes play an important role in the fruit coloring process. VvANS4, VvANS66 and VvANS113 had the highest expression levels in the full maturity stage. Conclusions These results indicated that different members of VvANS gene family played a role in different coloring stages, and this study laid a foundation for further research on the function of ANS gene family.https://doi.org/10.1186/s12870-023-04648-3GrapeVvANS gene familyDifferent coloring stagesExpression analysis |
| spellingShingle | Yongqing Feng Xuechun Tian Wei Liang XinTong Nan Aoning Zhang Wenfang Li Zonghuan Ma Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages BMC Plant Biology Grape VvANS gene family Different coloring stages Expression analysis |
| title | Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages |
| title_full | Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages |
| title_fullStr | Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages |
| title_full_unstemmed | Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages |
| title_short | Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages |
| title_sort | genome wide identification of grape ans gene family and expression analysis at different fruit coloration stages |
| topic | Grape VvANS gene family Different coloring stages Expression analysis |
| url | https://doi.org/10.1186/s12870-023-04648-3 |
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