Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis
Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica inf...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2002-10-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000900024 |
| _version_ | 1797703100475113472 |
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| author | Nilton Thaumaturgo Mônica Magno Vilar Ricardo Edelenyi Miriam Tendler |
| author_facet | Nilton Thaumaturgo Mônica Magno Vilar Ricardo Edelenyi Miriam Tendler |
| author_sort | Nilton Thaumaturgo |
| collection | DOAJ |
| description | Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests. |
| first_indexed | 2024-03-12T04:59:32Z |
| format | Article |
| id | doaj.art-38cb6cce7dcb4a02b453a419f346fab7 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:59:32Z |
| publishDate | 2002-10-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-38cb6cce7dcb4a02b453a419f346fab72023-09-03T09:14:09ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602002-10-019711511610.1590/S0074-02762002000900024Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysisNilton ThaumaturgoMônica Magno VilarRicardo EdelenyiMiriam TendlerSm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts - Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata - presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000900024Sm14Schistosoma mansoniimmune crossreactionfatty acid-binding protein |
| spellingShingle | Nilton Thaumaturgo Mônica Magno Vilar Ricardo Edelenyi Miriam Tendler Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis Memorias do Instituto Oswaldo Cruz Sm14 Schistosoma mansoni immune crossreaction fatty acid-binding protein |
| title | Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis |
| title_full | Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis |
| title_fullStr | Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis |
| title_full_unstemmed | Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis |
| title_short | Characterization of Sm14 related components in different helminths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis |
| title_sort | characterization of sm14 related components in different helminths by sodium dodecyl sulphate polyacrylamide gel electrophoresis and western blotting analysis |
| topic | Sm14 Schistosoma mansoni immune crossreaction fatty acid-binding protein |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000900024 |
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