Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media
The interest in microorganisms to produce microbial lipids at large-scale processes has increased during the last decades. <i>Rhodosporidium toruloides</i>-1588 could be an efficient option for its ability to simultaneously utilize five- and six-carbon sugars. Nevertheless, one of the mo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/1/11 |
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| author | Carlos S. Osorio-González Rahul Saini Krishnamoorthy Hegde Satinder Kaur Brar Alain Lefebvre Antonio Avalos Ramírez |
| author_facet | Carlos S. Osorio-González Rahul Saini Krishnamoorthy Hegde Satinder Kaur Brar Alain Lefebvre Antonio Avalos Ramírez |
| author_sort | Carlos S. Osorio-González |
| collection | DOAJ |
| description | The interest in microorganisms to produce microbial lipids at large-scale processes has increased during the last decades. <i>Rhodosporidium toruloides</i>-1588 could be an efficient option for its ability to simultaneously utilize five- and six-carbon sugars. Nevertheless, one of the most important characteristics that any strain needs to be considered or used at an industrial scale is its capacity to grow in substrates with high sugar concentrations. In this study, the effect of high sugar concentrations and the effect of ammonium sulfate were tested on <i>R. toruloides</i>-1588 and its capacity to grow and accumulate lipids using undetoxified wood hydrolysates. Batch fermentations showed a catabolic repression effect on <i>R. toruloides</i>-1588 growth at sugar concentrations of 120 g/L. The maximum lipid accumulation was 8.2 g/L with palmitic, stearic, oleic, linoleic, and lignoceric acids as predominant fatty acids in the produced lipids. Furthermore, <i>R. toruloides</i>-1588 was able to utilize up to 80% of the total xylose content. Additionally, this study is the first to report the effect of using high xylose concentrations on the growth, sugar utilization, and lipid accumulation by <i>R. toruloides</i>-1588. |
| first_indexed | 2024-03-09T12:47:45Z |
| format | Article |
| id | doaj.art-38ccce4c50c3445881b82207c30d79f8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T12:47:45Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-38ccce4c50c3445881b82207c30d79f82023-11-30T22:10:11ZengMDPI AGFermentation2311-56372022-12-01911110.3390/fermentation9010011Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture MediaCarlos S. Osorio-González0Rahul Saini1Krishnamoorthy Hegde2Satinder Kaur Brar3Alain Lefebvre4Antonio Avalos Ramírez5Department of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON M3J 1P3, CanadaDepartment of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON M3J 1P3, CanadaDepartment of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON M3J 1P3, CanadaDepartment of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON M3J 1P3, CanadaGreenfield Ethanol Quebec, 3300 Rte Marie-Victorin, Varennes, QC J3X 1P7, CanadaCentre National en Électrochimie et en Technologies Environnementales, 2263, Avenue du Collège, Shawinigan, QC G9N 6V8, CanadaThe interest in microorganisms to produce microbial lipids at large-scale processes has increased during the last decades. <i>Rhodosporidium toruloides</i>-1588 could be an efficient option for its ability to simultaneously utilize five- and six-carbon sugars. Nevertheless, one of the most important characteristics that any strain needs to be considered or used at an industrial scale is its capacity to grow in substrates with high sugar concentrations. In this study, the effect of high sugar concentrations and the effect of ammonium sulfate were tested on <i>R. toruloides</i>-1588 and its capacity to grow and accumulate lipids using undetoxified wood hydrolysates. Batch fermentations showed a catabolic repression effect on <i>R. toruloides</i>-1588 growth at sugar concentrations of 120 g/L. The maximum lipid accumulation was 8.2 g/L with palmitic, stearic, oleic, linoleic, and lignoceric acids as predominant fatty acids in the produced lipids. Furthermore, <i>R. toruloides</i>-1588 was able to utilize up to 80% of the total xylose content. Additionally, this study is the first to report the effect of using high xylose concentrations on the growth, sugar utilization, and lipid accumulation by <i>R. toruloides</i>-1588.https://www.mdpi.com/2311-5637/9/1/11substrate inhibitiongrowth inhibitionwood hydrolysatelipid production<i>Rhodosporidium toruloides</i> |
| spellingShingle | Carlos S. Osorio-González Rahul Saini Krishnamoorthy Hegde Satinder Kaur Brar Alain Lefebvre Antonio Avalos Ramírez Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media Fermentation substrate inhibition growth inhibition wood hydrolysate lipid production <i>Rhodosporidium toruloides</i> |
| title | Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media |
| title_full | Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media |
| title_fullStr | Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media |
| title_full_unstemmed | Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media |
| title_short | Crabtree Effect on <i>Rhodosporidium toruloides</i> Using Wood Hydrolysate as a Culture Media |
| title_sort | crabtree effect on i rhodosporidium toruloides i using wood hydrolysate as a culture media |
| topic | substrate inhibition growth inhibition wood hydrolysate lipid production <i>Rhodosporidium toruloides</i> |
| url | https://www.mdpi.com/2311-5637/9/1/11 |
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