The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus
Background. Viral infections trigger transcription factors, including Nrf2, which regulate the expression of genes related to cytokines, chemokines, and more. Nuclear factor erythroid 2-related factor 2 (Nrf2) participates in complex regulatory networks controlling the expression of cytoprotective g...
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| Format: | Article |
| Language: | English |
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Igor Sikorsky Kyiv Polytechnic Institute
2023-12-01
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| Series: | Innovative Biosystems and Bioengineering |
| Subjects: | |
| Online Access: | http://ibb.kpi.ua/article/view/290103 |
| _version_ | 1797356590605533184 |
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| author | Maryna Arkhypova Oleg Deriabin Tetiana Trokhymchuk Daria Starosyla Viktor Atamaniuk Michael Zavelevich Zhanna Vialykh Svitlana Rybalko Alexander Galkin |
| author_facet | Maryna Arkhypova Oleg Deriabin Tetiana Trokhymchuk Daria Starosyla Viktor Atamaniuk Michael Zavelevich Zhanna Vialykh Svitlana Rybalko Alexander Galkin |
| author_sort | Maryna Arkhypova |
| collection | DOAJ |
| description | Background. Viral infections trigger transcription factors, including Nrf2, which regulate the expression of genes related to cytokines, chemokines, and more. Nuclear factor erythroid 2-related factor 2 (Nrf2) participates in complex regulatory networks controlling the expression of cytoprotective genes and immune responses. Recently, new roles have been attributed to Nrf2, including the regulation of antiviral responses.
Objective. The aim of the study was to analyze Nrf2 activation in influenza virus infection in vivo and in transmissible gastroenteritis coronavirus (TGEV) infection in vitro, as well as the effects of the flavonoid compositions Proteflazidum® and Protoil on the Nrf2 expression in these two experimental models of viral infection.
Methods. Outbred white mice were infected with influenza A virus (A/FM/1/47/H1N1 strain). Jurkat cells were infected with TGEV, previously adapted to these cells. Flavonoid compositions Proteflazidum® and Protoil (Ecopharm, Ukraine), containing the mixture of tricin, luteolin, apigenin, quercetin, and rhamnosin, were used in corresponding dilutions. Real-Time PCR was employed to analyze Nrf2 RNA expression in the lungs of mice and in both uninfected and virus-infected cells. Additionally, ELISA was used to assess the expression of Nrf2 peptide.
Results. The Nrf2 expression in the lungs of influenza virus-infected mice showed a tendency to increase within a 100-fold range. In virus-infected mice treated with Proteflazidum® or Protoil, the level of Nrf2 expression in the lungs decreased about 10-fold compared to infected untreated mice. TGEV infection resulted in 100-fold increase in Nrf2 expression in Jurkat cells. Both Proteflazidum® and Protoil decreased Nrf2 expression in TGEV-infected cells, while their effects on Nrf2 expression in the intact cells on Day 1 were not detected.
Conclusions. Flavonoid compositions have only a slight effect on Nrf2 expression in intact cells. However, in case of virus infection, both in vivo and in vitro, they counteract the extensive up-regulation of Nrf2 expression due to viral infection. |
| first_indexed | 2024-03-08T14:29:53Z |
| format | Article |
| id | doaj.art-38ce126aa7a74ed6aa74c5c3a77db878 |
| institution | Directory Open Access Journal |
| issn | 2616-177X |
| language | English |
| last_indexed | 2024-03-08T14:29:53Z |
| publishDate | 2023-12-01 |
| publisher | Igor Sikorsky Kyiv Polytechnic Institute |
| record_format | Article |
| series | Innovative Biosystems and Bioengineering |
| spelling | doaj.art-38ce126aa7a74ed6aa74c5c3a77db8782024-01-12T12:17:15ZengIgor Sikorsky Kyiv Polytechnic InstituteInnovative Biosystems and Bioengineering2616-177X2023-12-0174485610.20535/ibb.2023.7.4.290103328382The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis CoronavirusMaryna Arkhypova0https://orcid.org/0000-0001-5860-2870Oleg Deriabin1https://orcid.org/0000-0001-5702-0190Tetiana Trokhymchuk2https://orcid.org/0009-0008-8208-0855Daria Starosyla3https://orcid.org/0000-0003-0210-2361Viktor Atamaniuk4https://orcid.org/0000-0002-8591-1633Michael Zavelevich5https://orcid.org/0000-0001-6778-7205Zhanna Vialykh6https://orcid.org/0009-0003-0977-917XSvitlana Rybalko7https://orcid.org/0000-0002-1913-1380Alexander Galkin8https://orcid.org/0000-0002-5309-6099L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases, National Academy of Medical Sciences of Ukraine; Igor Sikorsky Kyiv Polytechnic InstituteState Scientific Control Institute of Biotechnology and Strains of MicroorganismsL.V. Gromashevsky Institute of Epidemiology and Infectious Diseases, National Academy of Medical Sciences of UkraineL.V. Gromashevsky Institute of Epidemiology and Infectious Diseases, National Academy of Medical Sciences of UkraineEcopharm Research and Production Company, Research and Development UnitR.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of UkraineMain Medical Center of the Ministry of Internal Affairs of UkraineL.V. Gromashevsky Institute of Epidemiology and Infectious Diseases, National Academy of Medical Sciences of UkraineIgor Sikorsky Kyiv Polytechnic InstituteBackground. Viral infections trigger transcription factors, including Nrf2, which regulate the expression of genes related to cytokines, chemokines, and more. Nuclear factor erythroid 2-related factor 2 (Nrf2) participates in complex regulatory networks controlling the expression of cytoprotective genes and immune responses. Recently, new roles have been attributed to Nrf2, including the regulation of antiviral responses. Objective. The aim of the study was to analyze Nrf2 activation in influenza virus infection in vivo and in transmissible gastroenteritis coronavirus (TGEV) infection in vitro, as well as the effects of the flavonoid compositions Proteflazidum® and Protoil on the Nrf2 expression in these two experimental models of viral infection. Methods. Outbred white mice were infected with influenza A virus (A/FM/1/47/H1N1 strain). Jurkat cells were infected with TGEV, previously adapted to these cells. Flavonoid compositions Proteflazidum® and Protoil (Ecopharm, Ukraine), containing the mixture of tricin, luteolin, apigenin, quercetin, and rhamnosin, were used in corresponding dilutions. Real-Time PCR was employed to analyze Nrf2 RNA expression in the lungs of mice and in both uninfected and virus-infected cells. Additionally, ELISA was used to assess the expression of Nrf2 peptide. Results. The Nrf2 expression in the lungs of influenza virus-infected mice showed a tendency to increase within a 100-fold range. In virus-infected mice treated with Proteflazidum® or Protoil, the level of Nrf2 expression in the lungs decreased about 10-fold compared to infected untreated mice. TGEV infection resulted in 100-fold increase in Nrf2 expression in Jurkat cells. Both Proteflazidum® and Protoil decreased Nrf2 expression in TGEV-infected cells, while their effects on Nrf2 expression in the intact cells on Day 1 were not detected. Conclusions. Flavonoid compositions have only a slight effect on Nrf2 expression in intact cells. However, in case of virus infection, both in vivo and in vitro, they counteract the extensive up-regulation of Nrf2 expression due to viral infection.http://ibb.kpi.ua/article/view/290103nrf2flavonoidsinfluenza virustransmissible gastroenteritis coronavirus |
| spellingShingle | Maryna Arkhypova Oleg Deriabin Tetiana Trokhymchuk Daria Starosyla Viktor Atamaniuk Michael Zavelevich Zhanna Vialykh Svitlana Rybalko Alexander Galkin The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus Innovative Biosystems and Bioengineering nrf2 flavonoids influenza virus transmissible gastroenteritis coronavirus |
| title | The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus |
| title_full | The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus |
| title_fullStr | The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus |
| title_full_unstemmed | The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus |
| title_short | The Influence of Flavonoid Compositions on Nrf2 Transcription Factor Expression in Case of Infections Triggered by Influenza A Virus and Transmissible Gastroenteritis Coronavirus |
| title_sort | influence of flavonoid compositions on nrf2 transcription factor expression in case of infections triggered by influenza a virus and transmissible gastroenteritis coronavirus |
| topic | nrf2 flavonoids influenza virus transmissible gastroenteritis coronavirus |
| url | http://ibb.kpi.ua/article/view/290103 |
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