Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil
In the semiarid of the state of Paraíba, the anti-rabies vaccination is not common, most of the local inhabitants who deal with the animals do not know the incidence of the disease in the region. In this study, samples of foxes (Pseudalopex vetulus), insectivorous bats (Molossus molossus), raccoons...
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Instituto Biológico
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| Series: | Arquivos do Instituto Biológico |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400020&lng=en&tlng=en |
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| author | A.A.B. Gomes M.L.C.R. Silva F. Bernardi T. Sakai T. Itou F.H. Ito |
| author_facet | A.A.B. Gomes M.L.C.R. Silva F. Bernardi T. Sakai T. Itou F.H. Ito |
| author_sort | A.A.B. Gomes |
| collection | DOAJ |
| description | In the semiarid of the state of Paraíba, the anti-rabies vaccination is not common, most of the local inhabitants who deal with the animals do not know the incidence of the disease in the region. In this study, samples of foxes (Pseudalopex vetulus), insectivorous bats (Molossus molossus), raccoons (Procyon cancrivorous) and domestic animals brains were submitted to the diagnosis of rabies, by using the direct fluorescent antibody technique (d-FAT) and mouse inoculation test (MIT). Of the 581 examined materials, 50 (8.60 %) were positive for d-FAT and 47 (8.09 %) for MIT. From the positive samples for rabies, RNAs were extracted and transformed to cDNA, at the Laboratory of Rabies/Faculdade de Medicina Veterinária e Zootecnia/USP, SP. The phylogenetic characterization of the N gene was performed at the Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade Nihon, Faculdade de Ciências Bioresource, Fujisawa, Kanagawa, Japão. Based on the results of genotyping and phylogenetic analyzes, it is concluded that the epidemiology of rabies is complex in the semiarid of Paraíba, with different viral variants being maintained in domestic dogs, foxes, insectivorous bats and vampire bats. All the isolates examined belong to the genotype I of the genus Lyssavirus and it is possible to state that in the region, foxes are important sylvatic reservoirs of the rabies virus. |
| first_indexed | 2024-12-17T12:47:45Z |
| format | Article |
| id | doaj.art-38d1437c8d3449959e0c1dd63c99826a |
| institution | Directory Open Access Journal |
| issn | 0020-3653 1808-1657 |
| language | English |
| last_indexed | 2024-12-17T12:47:45Z |
| publisher | Instituto Biológico |
| record_format | Article |
| series | Arquivos do Instituto Biológico |
| spelling | doaj.art-38d1437c8d3449959e0c1dd63c99826a2022-12-21T21:47:41ZengInstituto BiológicoArquivos do Instituto Biológico0020-36531808-1657794611615S1808-16572012000400020Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern BrazilA.A.B. Gomes0M.L.C.R. Silva1F. Bernardi2T. Sakai3T. Itou4F.H. Ito5Universidade Federal de Campina GrandeUniversidade Federal de Campina GrandeCentro de Vigilância Sanitária e Controle de Zoonoses Tereza Rodrigues de CamargoUniversidade NihonUniversidade NihonUniversidade de São PauloIn the semiarid of the state of Paraíba, the anti-rabies vaccination is not common, most of the local inhabitants who deal with the animals do not know the incidence of the disease in the region. In this study, samples of foxes (Pseudalopex vetulus), insectivorous bats (Molossus molossus), raccoons (Procyon cancrivorous) and domestic animals brains were submitted to the diagnosis of rabies, by using the direct fluorescent antibody technique (d-FAT) and mouse inoculation test (MIT). Of the 581 examined materials, 50 (8.60 %) were positive for d-FAT and 47 (8.09 %) for MIT. From the positive samples for rabies, RNAs were extracted and transformed to cDNA, at the Laboratory of Rabies/Faculdade de Medicina Veterinária e Zootecnia/USP, SP. The phylogenetic characterization of the N gene was performed at the Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Universidade Nihon, Faculdade de Ciências Bioresource, Fujisawa, Kanagawa, Japão. Based on the results of genotyping and phylogenetic analyzes, it is concluded that the epidemiology of rabies is complex in the semiarid of Paraíba, with different viral variants being maintained in domestic dogs, foxes, insectivorous bats and vampire bats. All the isolates examined belong to the genotype I of the genus Lyssavirus and it is possible to state that in the region, foxes are important sylvatic reservoirs of the rabies virus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400020&lng=en&tlng=enlyssaviruswild canidsdomestic animalinsectivorous bats |
| spellingShingle | A.A.B. Gomes M.L.C.R. Silva F. Bernardi T. Sakai T. Itou F.H. Ito Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil Arquivos do Instituto Biológico lyssavirus wild canids domestic animal insectivorous bats |
| title | Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil |
| title_full | Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil |
| title_fullStr | Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil |
| title_full_unstemmed | Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil |
| title_short | Molecular epidemiology of animal rabies in the semiarid region of Paraíba, Northeastern Brazil |
| title_sort | molecular epidemiology of animal rabies in the semiarid region of paraiba northeastern brazil |
| topic | lyssavirus wild canids domestic animal insectivorous bats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572012000400020&lng=en&tlng=en |
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