The relationship between H19 and parameters of ovarian reserve
Abstract Context The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Anti-mullerian hormone (Amh) appears to be regulated by H19. However, the relationshi...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-020-00578-z |
| _version_ | 1818963390009180160 |
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| author | Xi Xia Martina S. Burn Yong Chen Cengiz Karakaya Amanda Kallen |
| author_facet | Xi Xia Martina S. Burn Yong Chen Cengiz Karakaya Amanda Kallen |
| author_sort | Xi Xia |
| collection | DOAJ |
| description | Abstract Context The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Anti-mullerian hormone (Amh) appears to be regulated by H19. However, the relationship between circulating H19 and markers of ovarian reserve including AMH not been investigated. Objective To determine whether H19 expression is altered in women with decreased ovarian reserve. Design Experimental study. Setting Yale School of Medicine (New Haven, USA) and Gazi University School of Medicine (Ankara, Turkey). Patients or other participants A total of 141 women undergoing infertility evaluation and treatment. Intervention Collection of discarded blood samples and cumulus cells at the time of baseline infertility evaluation and transvaginal oocyte retrieval, respectively. Main outcome measure Serum and cumulus cell H19 expression. Results Women with diminished ovarian reserve (as determined by AMH) had significantly lower serum H19 expression levels as compared to controls (p < 0.01). Serum H19 was moderately positively correlated with serum AMH. H19 expression was increased 3.7-fold in cumulus cells of IVF patients who demonstrated a high response to gonadotropins, compared to low responders (p < 0.05). Conclusion In this study, we show that downregulation of H19 in serum and cumulus cells is closely associated with decreased ovarian reserve, as measured by decreased AMH levels and reduced oocyte yield at oocyte retrieval. Further study with expanded sample sizes is necessary to determine whether H19 may be of use as a novel biomarker for diminished ovarian reserve. |
| first_indexed | 2024-12-20T12:44:27Z |
| format | Article |
| id | doaj.art-38d2dd799cc0437589b5ca61a485a8b8 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-20T12:44:27Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-38d2dd799cc0437589b5ca61a485a8b82022-12-21T19:40:22ZengBMCReproductive Biology and Endocrinology1477-78272020-05-011811810.1186/s12958-020-00578-zThe relationship between H19 and parameters of ovarian reserveXi Xia0Martina S. Burn1Yong Chen2Cengiz Karakaya3Amanda Kallen4Reproductive Center, Peking University Shenzhen HospitalDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics, Yale School of Medicine, Gynecology, and Reproductive SciencesDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics, Yale School of Medicine, Gynecology, and Reproductive SciencesDepartment of Medical Biochemistry, Gazi University School of MedicineDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics, Yale School of Medicine, Gynecology, and Reproductive SciencesAbstract Context The H19 long noncoding RNA (lncRNA) belongs to a highly conserved, imprinted gene cluster involved in embryonic development and growth control. We previously described a novel mechanism whereby the Anti-mullerian hormone (Amh) appears to be regulated by H19. However, the relationship between circulating H19 and markers of ovarian reserve including AMH not been investigated. Objective To determine whether H19 expression is altered in women with decreased ovarian reserve. Design Experimental study. Setting Yale School of Medicine (New Haven, USA) and Gazi University School of Medicine (Ankara, Turkey). Patients or other participants A total of 141 women undergoing infertility evaluation and treatment. Intervention Collection of discarded blood samples and cumulus cells at the time of baseline infertility evaluation and transvaginal oocyte retrieval, respectively. Main outcome measure Serum and cumulus cell H19 expression. Results Women with diminished ovarian reserve (as determined by AMH) had significantly lower serum H19 expression levels as compared to controls (p < 0.01). Serum H19 was moderately positively correlated with serum AMH. H19 expression was increased 3.7-fold in cumulus cells of IVF patients who demonstrated a high response to gonadotropins, compared to low responders (p < 0.05). Conclusion In this study, we show that downregulation of H19 in serum and cumulus cells is closely associated with decreased ovarian reserve, as measured by decreased AMH levels and reduced oocyte yield at oocyte retrieval. Further study with expanded sample sizes is necessary to determine whether H19 may be of use as a novel biomarker for diminished ovarian reserve.http://link.springer.com/article/10.1186/s12958-020-00578-zH19Noncoding RNAncRNAOvarian reserveAMH |
| spellingShingle | Xi Xia Martina S. Burn Yong Chen Cengiz Karakaya Amanda Kallen The relationship between H19 and parameters of ovarian reserve Reproductive Biology and Endocrinology H19 Noncoding RNA ncRNA Ovarian reserve AMH |
| title | The relationship between H19 and parameters of ovarian reserve |
| title_full | The relationship between H19 and parameters of ovarian reserve |
| title_fullStr | The relationship between H19 and parameters of ovarian reserve |
| title_full_unstemmed | The relationship between H19 and parameters of ovarian reserve |
| title_short | The relationship between H19 and parameters of ovarian reserve |
| title_sort | relationship between h19 and parameters of ovarian reserve |
| topic | H19 Noncoding RNA ncRNA Ovarian reserve AMH |
| url | http://link.springer.com/article/10.1186/s12958-020-00578-z |
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