Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression
Abstract Objective The use of molecular markers can identify a subgroup of tumors with distinct recurrence patterns. The present study aimed to characterize the immunohistochemical expression of vimentin (VIM), of E-cadherin (CDH1), and of cytokeratin 5 (CK5) in patients with invasive ductal carci...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
2018-12-01
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| Series: | Revista Brasileira de Ginecologia e Obstetrícia |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/rbgo/v40n12/0100-7203-rbgo-40-12-00779.pdf |
| _version_ | 1798026355780091904 |
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| author | Leonardo Fleury Orlandini Francisco José Cândido dos Reis Willian Abraham da Silveira Marcelo Guimarães Tiezzi Jurandyr Moreira de Andrade Alfredo Ribeiro-Silva Ryan Deaton Maarten Bosland Daniel Guimarães Tiezzi |
| author_facet | Leonardo Fleury Orlandini Francisco José Cândido dos Reis Willian Abraham da Silveira Marcelo Guimarães Tiezzi Jurandyr Moreira de Andrade Alfredo Ribeiro-Silva Ryan Deaton Maarten Bosland Daniel Guimarães Tiezzi |
| author_sort | Leonardo Fleury Orlandini |
| collection | DOAJ |
| description | Abstract Objective The use of molecular markers can identify a subgroup of tumors with distinct recurrence patterns. The present study aimed to characterize the immunohistochemical expression of vimentin (VIM), of E-cadherin (CDH1), and of cytokeratin 5 (CK5) in patients with invasive ductal carcinomas (IDCs). Methods We have constructed a tissuemicroarray (TMA) from87 patients with IDC of the breast. Immunohistochemistry (IHC) was performed to study the expression of estrogen and progesterone receptors (ER and PgR), human epidermal growth factor receptor 2 (HER2), VIM, CDH1, CK5, and Ki67. The tumors were classified as luminal A and B (n = 39), HER2 enriched (n = 25), and triple-negative (TNBC) (n = 23), based on the IHC expression. Results We have observed that luminal A and B tumors lack the VIM+/CDH1-/low phenotype. This phenotype was observed in 16.5% of the HER2+ tumors and in 60% of the TNBC tumors (p = 0.0001). Out of a total of 20 TNBC tumors, the CK5 (basal-like marker) was positive in 11 of them. The VIM+/CDH1-/low phenotype was observed in 5 CK5+ TNBC tumors (45%) and in 7 out of 9 CK5- TNBC tumors (78%) (p = 0.02). The median Ki67 index in the VIM+/CDH1-/low tumors was 13.6 (range: 17.8-45.4) compared with 9.8 (range: 4.1-38.1) in other tumors (p = 0.0007). The presence of lymph nodemetastasis was less frequent in patients with VIM+/CDH1-/low tumors (23% versus 61%; X2 test; p = 0.01). Conclusion Our findings suggest that the expression of VIM and CDH1 can identify a subset of IDCs of the breast with a mesenchymal phenotype associated with poor prognosis, high-grade lesion, and high mitotic index. |
| first_indexed | 2024-04-11T18:35:12Z |
| format | Article |
| id | doaj.art-38d3be9d43154038861271cac7463314 |
| institution | Directory Open Access Journal |
| issn | 0100-7203 |
| language | English |
| last_indexed | 2024-04-11T18:35:12Z |
| publishDate | 2018-12-01 |
| publisher | Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
| record_format | Article |
| series | Revista Brasileira de Ginecologia e Obstetrícia |
| spelling | doaj.art-38d3be9d43154038861271cac74633142022-12-22T04:09:19ZengFederação Brasileira das Sociedades de Ginecologia e ObstetríciaRevista Brasileira de Ginecologia e Obstetrícia0100-72032018-12-01401277978610.1055/s-0038-1673700Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin ExpressionLeonardo Fleury OrlandiniFrancisco José Cândido dos ReisWillian Abraham da SilveiraMarcelo Guimarães TiezziJurandyr Moreira de AndradeAlfredo Ribeiro-SilvaRyan DeatonMaarten BoslandDaniel Guimarães TiezziAbstract Objective The use of molecular markers can identify a subgroup of tumors with distinct recurrence patterns. The present study aimed to characterize the immunohistochemical expression of vimentin (VIM), of E-cadherin (CDH1), and of cytokeratin 5 (CK5) in patients with invasive ductal carcinomas (IDCs). Methods We have constructed a tissuemicroarray (TMA) from87 patients with IDC of the breast. Immunohistochemistry (IHC) was performed to study the expression of estrogen and progesterone receptors (ER and PgR), human epidermal growth factor receptor 2 (HER2), VIM, CDH1, CK5, and Ki67. The tumors were classified as luminal A and B (n = 39), HER2 enriched (n = 25), and triple-negative (TNBC) (n = 23), based on the IHC expression. Results We have observed that luminal A and B tumors lack the VIM+/CDH1-/low phenotype. This phenotype was observed in 16.5% of the HER2+ tumors and in 60% of the TNBC tumors (p = 0.0001). Out of a total of 20 TNBC tumors, the CK5 (basal-like marker) was positive in 11 of them. The VIM+/CDH1-/low phenotype was observed in 5 CK5+ TNBC tumors (45%) and in 7 out of 9 CK5- TNBC tumors (78%) (p = 0.02). The median Ki67 index in the VIM+/CDH1-/low tumors was 13.6 (range: 17.8-45.4) compared with 9.8 (range: 4.1-38.1) in other tumors (p = 0.0007). The presence of lymph nodemetastasis was less frequent in patients with VIM+/CDH1-/low tumors (23% versus 61%; X2 test; p = 0.01). Conclusion Our findings suggest that the expression of VIM and CDH1 can identify a subset of IDCs of the breast with a mesenchymal phenotype associated with poor prognosis, high-grade lesion, and high mitotic index.http://www.scielo.br/pdf/rbgo/v40n12/0100-7203-rbgo-40-12-00779.pdfbreast cancerepithelialmesenchymal transitionvimentine-cadherinprognostic factors |
| spellingShingle | Leonardo Fleury Orlandini Francisco José Cândido dos Reis Willian Abraham da Silveira Marcelo Guimarães Tiezzi Jurandyr Moreira de Andrade Alfredo Ribeiro-Silva Ryan Deaton Maarten Bosland Daniel Guimarães Tiezzi Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression Revista Brasileira de Ginecologia e Obstetrícia breast cancer epithelialmesenchymal transition vimentin e-cadherin prognostic factors |
| title | Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression |
| title_full | Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression |
| title_fullStr | Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression |
| title_full_unstemmed | Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression |
| title_short | Identification of a Subtype of Poorly Differentiated Invasive Ductal Carcinoma of the Breast Based on Vimentin and E-cadherin Expression |
| title_sort | identification of a subtype of poorly differentiated invasive ductal carcinoma of the breast based on vimentin and e cadherin expression |
| topic | breast cancer epithelialmesenchymal transition vimentin e-cadherin prognostic factors |
| url | http://www.scielo.br/pdf/rbgo/v40n12/0100-7203-rbgo-40-12-00779.pdf |
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