The ets-related transcription factor GABP directs bidirectional transcription.
Approximately 10% of genes in the human genome are distributed such that their transcription start sites are located less than 1 kb apart on opposite strands. These divergent gene pairs have a single intergenic segment of DNA, which in some cases appears to share regulatory elements, but it is uncle...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-11-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2077898?pdf=render |
| _version_ | 1811267366383779840 |
|---|---|
| author | Patrick J Collins Yuya Kobayashi Loan Nguyen Nathan D Trinklein Richard M Myers |
| author_facet | Patrick J Collins Yuya Kobayashi Loan Nguyen Nathan D Trinklein Richard M Myers |
| author_sort | Patrick J Collins |
| collection | DOAJ |
| description | Approximately 10% of genes in the human genome are distributed such that their transcription start sites are located less than 1 kb apart on opposite strands. These divergent gene pairs have a single intergenic segment of DNA, which in some cases appears to share regulatory elements, but it is unclear whether these regions represent functional bidirectional promoters or two overlapping promoters. A recent study showed that divergent promoters are enriched for consensus binding sequences of a small group of transcription factors, including the ubiquitous ets-family transcription factor GA-binding protein (GABP). Here we show that GABP binds to more than 80% of divergent promoters in at least one cell type. Furthermore, we demonstrate that GABP binding is correlated and associated with bidirectional transcriptional activity in a luciferase transfection assay. In addition, we find that the addition of a strict consensus GABP site into a set of promoters that normally function in only one direction significantly increases activity in the opposite direction in 67% of cases. Our findings demonstrate that GABP regulates the majority of divergent promoters and suggest that bidirectional transcriptional activity is mediated through GABP binding and transactivation at both divergent and nondivergent promoters. |
| first_indexed | 2024-04-12T21:00:06Z |
| format | Article |
| id | doaj.art-38d40e69faaf49debdbd62aaccc3b4d5 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T21:00:06Z |
| publishDate | 2007-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-38d40e69faaf49debdbd62aaccc3b4d52022-12-22T03:16:51ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042007-11-01311e20810.1371/journal.pgen.0030208The ets-related transcription factor GABP directs bidirectional transcription.Patrick J CollinsYuya KobayashiLoan NguyenNathan D TrinkleinRichard M MyersApproximately 10% of genes in the human genome are distributed such that their transcription start sites are located less than 1 kb apart on opposite strands. These divergent gene pairs have a single intergenic segment of DNA, which in some cases appears to share regulatory elements, but it is unclear whether these regions represent functional bidirectional promoters or two overlapping promoters. A recent study showed that divergent promoters are enriched for consensus binding sequences of a small group of transcription factors, including the ubiquitous ets-family transcription factor GA-binding protein (GABP). Here we show that GABP binds to more than 80% of divergent promoters in at least one cell type. Furthermore, we demonstrate that GABP binding is correlated and associated with bidirectional transcriptional activity in a luciferase transfection assay. In addition, we find that the addition of a strict consensus GABP site into a set of promoters that normally function in only one direction significantly increases activity in the opposite direction in 67% of cases. Our findings demonstrate that GABP regulates the majority of divergent promoters and suggest that bidirectional transcriptional activity is mediated through GABP binding and transactivation at both divergent and nondivergent promoters.http://europepmc.org/articles/PMC2077898?pdf=render |
| spellingShingle | Patrick J Collins Yuya Kobayashi Loan Nguyen Nathan D Trinklein Richard M Myers The ets-related transcription factor GABP directs bidirectional transcription. PLoS Genetics |
| title | The ets-related transcription factor GABP directs bidirectional transcription. |
| title_full | The ets-related transcription factor GABP directs bidirectional transcription. |
| title_fullStr | The ets-related transcription factor GABP directs bidirectional transcription. |
| title_full_unstemmed | The ets-related transcription factor GABP directs bidirectional transcription. |
| title_short | The ets-related transcription factor GABP directs bidirectional transcription. |
| title_sort | ets related transcription factor gabp directs bidirectional transcription |
| url | http://europepmc.org/articles/PMC2077898?pdf=render |
| work_keys_str_mv | AT patrickjcollins theetsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT yuyakobayashi theetsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT loannguyen theetsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT nathandtrinklein theetsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT richardmmyers theetsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT patrickjcollins etsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT yuyakobayashi etsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT loannguyen etsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT nathandtrinklein etsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription AT richardmmyers etsrelatedtranscriptionfactorgabpdirectsbidirectionaltranscription |