Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy
Mounting evidence suggests that changes in microbiome are linked to development of cancer and its aggressiveness. Microbiome profiles in human breast tissue previously presumed to be sterile, have recently been characterized using high-throughput technologies. Recent findings of microbiome variation...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2018.00318/full |
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| author | Shen Meng Shen Meng Bin Chen Junjie Yang Jingwen Wang Dequan Zhu Qingsong Meng Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang |
| author_facet | Shen Meng Shen Meng Bin Chen Junjie Yang Jingwen Wang Dequan Zhu Qingsong Meng Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang |
| author_sort | Shen Meng |
| collection | DOAJ |
| description | Mounting evidence suggests that changes in microbiome are linked to development of cancer and its aggressiveness. Microbiome profiles in human breast tissue previously presumed to be sterile, have recently been characterized using high-throughput technologies. Recent findings of microbiome variation between benign and malignant disease provides a rationale for exploring microbiomes associated with cancer during tumor progression. We assessed microbiomes of aseptically collected human breast tissue samples in this study, using needle biopsy from patients with benign and malignant tumors of different histological grading, using 16S rRNA gene amplicon sequencing. This is consistent with previous studies, and our results identified distinct microbiome profiles in breast tissues from women with cancer as compared to women with benign breast disease in Chinese cohorts. The enriched microbial biomarkers in malignant tissue included genus Propionicimonas and families Micrococcaceae, Caulobacteraceae, Rhodobacteraceae, Nocardioidaceae, Methylobacteriaceae, which appeared to be ethno-specific. Further, we compared microbiome profiles in malignant tissues of three different histological grades. The relative abundance of family Bacteroidaceae decreased and that of genus Agrococcus increased with the development of malignancy. KEGG pathways inferred by PICRUSt showed that biotin and glycerophospholipid metabolism had significant differences in all three grades. Glycerophospholipid and ribosome biogenesis increased in grade III tissue as compared to grades I and II. Flavonoid biosynthesis significantly decreased in grade III tissue. The specific correlation of these potential microbial biomarkers and indicated pathways with advanced disease could have broad implications in the diagnosis and staging of breast cancer. Further large-cohort investigation of the breast cancer microbiome and its potential mechanism in breast cancer development are essential. |
| first_indexed | 2024-04-13T02:09:00Z |
| format | Article |
| id | doaj.art-38d554f3a46746559a1bc04e3bcff19b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-13T02:09:00Z |
| publishDate | 2018-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-38d554f3a46746559a1bc04e3bcff19b2022-12-22T03:07:21ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2018-08-01810.3389/fonc.2018.00318359928Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting MalignancyShen Meng0Shen Meng1Bin Chen2Junjie Yang3Jingwen Wang4Dequan Zhu5Qingsong Meng6Lei Zhang7Lei Zhang8Lei Zhang9Lei Zhang10Lei Zhang11School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Jinan, Jinan, ChinaShandong Cancer Hospital Affiliated to Shandong University, Jinan, ChinaCollege of Life Science, Shandong Normal University, Jinan, ChinaCollege of Life Science, Qilu Normal University, Jinan, ChinaCollege of Life Science, Shandong Normal University, Jinan, ChinaMicrobiological Laboratory, Lin Yi People's Hospital, Linyi, ChinaClinical Laboratory, Qianfoshan Hospital Affiliated to Shandong University, Jinan, ChinaMicrobiological Laboratory, Lin Yi People's Hospital, Linyi, ChinaShandong Children's Microbiome Center, Qilu Children's Hospital of Shandong University, Jinan, ChinaShandong Institutes for Food and Drug Control, Jinan, ChinaQingdao Human Microbiome Center, No. 2 Affiliated Hospital of Qingdao University, Qingdao, China0Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Chemistry and Environment, Beihang University, Beijing, ChinaMounting evidence suggests that changes in microbiome are linked to development of cancer and its aggressiveness. Microbiome profiles in human breast tissue previously presumed to be sterile, have recently been characterized using high-throughput technologies. Recent findings of microbiome variation between benign and malignant disease provides a rationale for exploring microbiomes associated with cancer during tumor progression. We assessed microbiomes of aseptically collected human breast tissue samples in this study, using needle biopsy from patients with benign and malignant tumors of different histological grading, using 16S rRNA gene amplicon sequencing. This is consistent with previous studies, and our results identified distinct microbiome profiles in breast tissues from women with cancer as compared to women with benign breast disease in Chinese cohorts. The enriched microbial biomarkers in malignant tissue included genus Propionicimonas and families Micrococcaceae, Caulobacteraceae, Rhodobacteraceae, Nocardioidaceae, Methylobacteriaceae, which appeared to be ethno-specific. Further, we compared microbiome profiles in malignant tissues of three different histological grades. The relative abundance of family Bacteroidaceae decreased and that of genus Agrococcus increased with the development of malignancy. KEGG pathways inferred by PICRUSt showed that biotin and glycerophospholipid metabolism had significant differences in all three grades. Glycerophospholipid and ribosome biogenesis increased in grade III tissue as compared to grades I and II. Flavonoid biosynthesis significantly decreased in grade III tissue. The specific correlation of these potential microbial biomarkers and indicated pathways with advanced disease could have broad implications in the diagnosis and staging of breast cancer. Further large-cohort investigation of the breast cancer microbiome and its potential mechanism in breast cancer development are essential.https://www.frontiersin.org/article/10.3389/fonc.2018.00318/fullmicrobiomebreast cancerneedle biopsymalignancypathogen |
| spellingShingle | Shen Meng Shen Meng Bin Chen Junjie Yang Jingwen Wang Dequan Zhu Qingsong Meng Lei Zhang Lei Zhang Lei Zhang Lei Zhang Lei Zhang Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy Frontiers in Oncology microbiome breast cancer needle biopsy malignancy pathogen |
| title | Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy |
| title_full | Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy |
| title_fullStr | Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy |
| title_full_unstemmed | Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy |
| title_short | Study of Microbiomes in Aseptically Collected Samples of Human Breast Tissue Using Needle Biopsy and the Potential Role of in situ Tissue Microbiomes for Promoting Malignancy |
| title_sort | study of microbiomes in aseptically collected samples of human breast tissue using needle biopsy and the potential role of in situ tissue microbiomes for promoting malignancy |
| topic | microbiome breast cancer needle biopsy malignancy pathogen |
| url | https://www.frontiersin.org/article/10.3389/fonc.2018.00318/full |
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