DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil
The substitution and mislabeling is facilitated by the processing of fish products. We employed a DNA barcoding to authenticate fillets labeled as “dourada” (Brachyplatystoma rousseauxii), and “piramutaba” (Brachyplatystoma vaillantii) marketed in the Brazil. A 615 bp of the Cytochrome oxidase subun...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-09-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402031731X |
| _version_ | 1818554739387793408 |
|---|---|
| author | Soraia Costa de Carvalho Iracilda Sampaio Simoni Santos |
| author_facet | Soraia Costa de Carvalho Iracilda Sampaio Simoni Santos |
| author_sort | Soraia Costa de Carvalho |
| collection | DOAJ |
| description | The substitution and mislabeling is facilitated by the processing of fish products. We employed a DNA barcoding to authenticate fillets labeled as “dourada” (Brachyplatystoma rousseauxii), and “piramutaba” (Brachyplatystoma vaillantii) marketed in the Brazil. A 615 bp of the Cytochrome oxidase subunit I (COI) was sequenced from 305 fillets and subsequently identified to species level by querying public databases and sequences of reference species. The results revealed a global mean substitution rate of 17%. The highest substitution rate was detected in “dourada” (26%), the most valuable species, followed by “piramutaba” (9%). The most cases of substitutions were by species of lower commercial value, suggesting fraud aimed at increased profits. Therefore, we suggest the improvement of food-labeling regulation, continued inspection, as well as the adoption of the DNA barcode for the molecular authentication of processed fish to prevent substitution of these products in Brazil. |
| first_indexed | 2024-12-12T09:44:03Z |
| format | Article |
| id | doaj.art-38d5b3ccd7624ec39eeace726d62c5eb |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-12T09:44:03Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-38d5b3ccd7624ec39eeace726d62c5eb2022-12-22T00:28:28ZengElsevierHeliyon2405-84402020-09-0169e04888DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in BrazilSoraia Costa de Carvalho0Iracilda Sampaio1Simoni Santos2Laboratory of Fish Microbiology, Institute of Coastal Studies, Federal University of Para, Alameda Leandro Ribeiro s/n, 68600-000, Braganca, Para, BrazilLaboratory of Genetics and Molecular Biology, Institute of Coastal Studies, Federal University of Para, Alameda Leandro Ribeiro s/n, 68600-000, Braganca, Para, BrazilLaboratory of Fish Microbiology, Institute of Coastal Studies, Federal University of Para, Alameda Leandro Ribeiro s/n, 68600-000, Braganca, Para, Brazil; Corresponding author.The substitution and mislabeling is facilitated by the processing of fish products. We employed a DNA barcoding to authenticate fillets labeled as “dourada” (Brachyplatystoma rousseauxii), and “piramutaba” (Brachyplatystoma vaillantii) marketed in the Brazil. A 615 bp of the Cytochrome oxidase subunit I (COI) was sequenced from 305 fillets and subsequently identified to species level by querying public databases and sequences of reference species. The results revealed a global mean substitution rate of 17%. The highest substitution rate was detected in “dourada” (26%), the most valuable species, followed by “piramutaba” (9%). The most cases of substitutions were by species of lower commercial value, suggesting fraud aimed at increased profits. Therefore, we suggest the improvement of food-labeling regulation, continued inspection, as well as the adoption of the DNA barcode for the molecular authentication of processed fish to prevent substitution of these products in Brazil.http://www.sciencedirect.com/science/article/pii/S240584402031731XFood scienceFood analysisIndustryDNA barcodingSpecies substitutionProcessed fish |
| spellingShingle | Soraia Costa de Carvalho Iracilda Sampaio Simoni Santos DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil Heliyon Food science Food analysis Industry DNA barcoding Species substitution Processed fish |
| title | DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil |
| title_full | DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil |
| title_fullStr | DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil |
| title_full_unstemmed | DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil |
| title_short | DNA barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus Brachyplatystoma Bleeker, 1862, the Amazonian freshwater catfishes economically important in Brazil |
| title_sort | dna barcoding reveals mislabeling and commercial fraud in the marketing of fillets of the genus brachyplatystoma bleeker 1862 the amazonian freshwater catfishes economically important in brazil |
| topic | Food science Food analysis Industry DNA barcoding Species substitution Processed fish |
| url | http://www.sciencedirect.com/science/article/pii/S240584402031731X |
| work_keys_str_mv | AT soraiacostadecarvalho dnabarcodingrevealsmislabelingandcommercialfraudinthemarketingoffilletsofthegenusbrachyplatystomableeker1862theamazonianfreshwatercatfisheseconomicallyimportantinbrazil AT iracildasampaio dnabarcodingrevealsmislabelingandcommercialfraudinthemarketingoffilletsofthegenusbrachyplatystomableeker1862theamazonianfreshwatercatfisheseconomicallyimportantinbrazil AT simonisantos dnabarcodingrevealsmislabelingandcommercialfraudinthemarketingoffilletsofthegenusbrachyplatystomableeker1862theamazonianfreshwatercatfisheseconomicallyimportantinbrazil |