Respostas imuno-celulares a antigénios micobacterianos.
The authors studied the immunological response before and after beginning anti-tuberculous therapy, of a previously health, HIV negative, 22 year old black male, from the Republic of Cape Verde. The patient had multiple vertebral bony lesions associated to subcutaneous abscesses. As immunological ma...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Ordem dos Médicos
1998-10-01
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| Series: | Acta Médica Portuguesa |
| Online Access: | https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2331 |
| _version_ | 1798021213269786624 |
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| author | D Ordway M F Moraes L Oliveira R Badura M N Pinheiro J M Graça L Carvalho T Saldanha P Abecasis F A Ventura |
| author_facet | D Ordway M F Moraes L Oliveira R Badura M N Pinheiro J M Graça L Carvalho T Saldanha P Abecasis F A Ventura |
| author_sort | D Ordway |
| collection | DOAJ |
| description | The authors studied the immunological response before and after beginning anti-tuberculous therapy, of a previously health, HIV negative, 22 year old black male, from the Republic of Cape Verde. The patient had multiple vertebral bony lesions associated to subcutaneous abscesses. As immunological markers of antigen recognition, we measured blastogenic and cytotoxic responses and gamma-IFN secretion towards 30 kD, 65 kD, filtrate proteins of M. tuberculosis, M. tuberculosis (H37Rv) and PPD cultures. To characterise the role of cytokines during infection, expression of mRNA for gamma-IFN, IL-4 and IL-10 was also analysed. A slight increase of lymphocyte proliferation and gamma-IFN production was seen in response to purified protein derivative (PPD) and short term culture filtrate proteins (ST-CFP), after one month of therapy. More significant, was the increase in M. tuberculosis and PPD-specific cytolytic T lymphocyte response after one one month of treatment. After 6 months of treatment, blastogenic and cytotoxic responses and gamma-IFN production were considerably higher toward the antigen panel. The CD4/CD8 ratio increased from 0.7 to 1.4 after treatment. We observed that ST-CFP and MT-CFP induced increasingly higher lymphoproliferation and gamma-IFN production, confirming their role in the protective immune responses to M. tuberculosis. The reduced immune responses in the peripheral blood of this patient probably reflect a high activity in the local sites of infection. This case of disseminated tuberculosis infection maybe related to nutritional or social factors or may represent an example of reduced in ate resistance against tuberculosis infection. |
| first_indexed | 2024-04-11T17:10:10Z |
| format | Article |
| id | doaj.art-38d6764b5c5844e9905f11f14250af81 |
| institution | Directory Open Access Journal |
| issn | 0870-399X 1646-0758 |
| language | English |
| last_indexed | 2024-04-11T17:10:10Z |
| publishDate | 1998-10-01 |
| publisher | Ordem dos Médicos |
| record_format | Article |
| series | Acta Médica Portuguesa |
| spelling | doaj.art-38d6764b5c5844e9905f11f14250af812022-12-22T04:12:55ZengOrdem dos MédicosActa Médica Portuguesa0870-399X1646-07581998-10-01111010.20344/amp.2331Respostas imuno-celulares a antigénios micobacterianos.D Ordway0M F MoraesL OliveiraR BaduraM N PinheiroJ M GraçaL CarvalhoT SaldanhaP AbecasisF A VenturaCentro de Malária e Outras Doenças Tropicais/IHMT e Hospital Egas Moniz, Lisboa.The authors studied the immunological response before and after beginning anti-tuberculous therapy, of a previously health, HIV negative, 22 year old black male, from the Republic of Cape Verde. The patient had multiple vertebral bony lesions associated to subcutaneous abscesses. As immunological markers of antigen recognition, we measured blastogenic and cytotoxic responses and gamma-IFN secretion towards 30 kD, 65 kD, filtrate proteins of M. tuberculosis, M. tuberculosis (H37Rv) and PPD cultures. To characterise the role of cytokines during infection, expression of mRNA for gamma-IFN, IL-4 and IL-10 was also analysed. A slight increase of lymphocyte proliferation and gamma-IFN production was seen in response to purified protein derivative (PPD) and short term culture filtrate proteins (ST-CFP), after one month of therapy. More significant, was the increase in M. tuberculosis and PPD-specific cytolytic T lymphocyte response after one one month of treatment. After 6 months of treatment, blastogenic and cytotoxic responses and gamma-IFN production were considerably higher toward the antigen panel. The CD4/CD8 ratio increased from 0.7 to 1.4 after treatment. We observed that ST-CFP and MT-CFP induced increasingly higher lymphoproliferation and gamma-IFN production, confirming their role in the protective immune responses to M. tuberculosis. The reduced immune responses in the peripheral blood of this patient probably reflect a high activity in the local sites of infection. This case of disseminated tuberculosis infection maybe related to nutritional or social factors or may represent an example of reduced in ate resistance against tuberculosis infection.https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2331 |
| spellingShingle | D Ordway M F Moraes L Oliveira R Badura M N Pinheiro J M Graça L Carvalho T Saldanha P Abecasis F A Ventura Respostas imuno-celulares a antigénios micobacterianos. Acta Médica Portuguesa |
| title | Respostas imuno-celulares a antigénios micobacterianos. |
| title_full | Respostas imuno-celulares a antigénios micobacterianos. |
| title_fullStr | Respostas imuno-celulares a antigénios micobacterianos. |
| title_full_unstemmed | Respostas imuno-celulares a antigénios micobacterianos. |
| title_short | Respostas imuno-celulares a antigénios micobacterianos. |
| title_sort | respostas imuno celulares a antigenios micobacterianos |
| url | https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2331 |
| work_keys_str_mv | AT dordway respostasimunocelularesaantigeniosmicobacterianos AT mfmoraes respostasimunocelularesaantigeniosmicobacterianos AT loliveira respostasimunocelularesaantigeniosmicobacterianos AT rbadura respostasimunocelularesaantigeniosmicobacterianos AT mnpinheiro respostasimunocelularesaantigeniosmicobacterianos AT jmgraca respostasimunocelularesaantigeniosmicobacterianos AT lcarvalho respostasimunocelularesaantigeniosmicobacterianos AT tsaldanha respostasimunocelularesaantigeniosmicobacterianos AT pabecasis respostasimunocelularesaantigeniosmicobacterianos AT faventura respostasimunocelularesaantigeniosmicobacterianos |