Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901
<i>Synechococcus</i> sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/7/872 |
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| author | Lauren A. Mills José Ángel Moreno-Cabezuelo Artur Włodarczyk Angelo J. Victoria Rebeca Mejías Anja Nenninger Simon Moxon Paolo Bombelli Tiago T. Selão Alistair J. McCormick David J. Lea-Smith |
| author_facet | Lauren A. Mills José Ángel Moreno-Cabezuelo Artur Włodarczyk Angelo J. Victoria Rebeca Mejías Anja Nenninger Simon Moxon Paolo Bombelli Tiago T. Selão Alistair J. McCormick David J. Lea-Smith |
| author_sort | Lauren A. Mills |
| collection | DOAJ |
| description | <i>Synechococcus</i> sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermore, tools specific for genetic manipulation of PCC 11901 are not established. Here, we demonstrate that PCC 11901 shows faster growth than other model cyanobacteria, including the fast-growing species <i>Synechococcus</i><i>elongatus</i> UTEX 2973, under optimal growth conditions for UTEX 2973. Comparative genomics between PCC 11901 and <i>Synechocystis</i> sp. PCC 6803 reveal conservation of most metabolic pathways but PCC 11901 has a simplified electron transport chain and reduced light harvesting complex. This may underlie its superior light use, reduced photoinhibition, and higher photosynthetic and respiratory rates. To aid biotechnology applications, we developed a vitamin B<sub>12</sub> auxotrophic mutant but were unable to generate unmarked knockouts using two negative selectable markers, suggesting that recombinase- or CRISPR-based approaches may be required for repeated genetic manipulation. Overall, this study establishes PCC 11901 as one of the most promising species currently available for cyanobacterial biotechnology and provides a useful set of bioinformatics tools and strains for advancing this field, in addition to insights into the factors underlying its fast growth phenotype. |
| first_indexed | 2024-03-09T03:39:13Z |
| format | Article |
| id | doaj.art-38d74810b3c143feb4298e1ddcd8227c |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T03:39:13Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-38d74810b3c143feb4298e1ddcd8227c2023-12-03T14:43:07ZengMDPI AGBiomolecules2218-273X2022-06-0112787210.3390/biom12070872Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901Lauren A. Mills0José Ángel Moreno-Cabezuelo1Artur Włodarczyk2Angelo J. Victoria3Rebeca Mejías4Anja Nenninger5Simon Moxon6Paolo Bombelli7Tiago T. Selão8Alistair J. McCormick9David J. Lea-Smith10School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKBondi Bio Pty Ltd., c/o Climate Change Cluster, University of Technology Sydney, 745 Harris Street, Ultimo, NSW 2007, AustraliaSynthSys and Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UKSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKSynthSys and Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UKSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UKDepartment of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UKDepartment of Chemical and Environmental Engineering, University of Nottingham, Nottingham NG7 2RD, UKSynthSys and Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UKSchool of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK<i>Synechococcus</i> sp. PCC 11901 reportedly demonstrates the highest, most sustained growth of any known cyanobacterium under optimized conditions. Due to its recent discovery, our knowledge of its biology, including the factors underlying sustained, fast growth, is limited. Furthermore, tools specific for genetic manipulation of PCC 11901 are not established. Here, we demonstrate that PCC 11901 shows faster growth than other model cyanobacteria, including the fast-growing species <i>Synechococcus</i><i>elongatus</i> UTEX 2973, under optimal growth conditions for UTEX 2973. Comparative genomics between PCC 11901 and <i>Synechocystis</i> sp. PCC 6803 reveal conservation of most metabolic pathways but PCC 11901 has a simplified electron transport chain and reduced light harvesting complex. This may underlie its superior light use, reduced photoinhibition, and higher photosynthetic and respiratory rates. To aid biotechnology applications, we developed a vitamin B<sub>12</sub> auxotrophic mutant but were unable to generate unmarked knockouts using two negative selectable markers, suggesting that recombinase- or CRISPR-based approaches may be required for repeated genetic manipulation. Overall, this study establishes PCC 11901 as one of the most promising species currently available for cyanobacterial biotechnology and provides a useful set of bioinformatics tools and strains for advancing this field, in addition to insights into the factors underlying its fast growth phenotype.https://www.mdpi.com/2218-273X/12/7/872<i>Synechococcus</i> sp. PCC 11901CodA selectionSacB selectionvitamin B12photosynthesisphotoinhibition |
| spellingShingle | Lauren A. Mills José Ángel Moreno-Cabezuelo Artur Włodarczyk Angelo J. Victoria Rebeca Mejías Anja Nenninger Simon Moxon Paolo Bombelli Tiago T. Selão Alistair J. McCormick David J. Lea-Smith Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 Biomolecules <i>Synechococcus</i> sp. PCC 11901 CodA selection SacB selection vitamin B12 photosynthesis photoinhibition |
| title | Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 |
| title_full | Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 |
| title_fullStr | Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 |
| title_full_unstemmed | Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 |
| title_short | Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium <em>Synechococcus</em> sp. PCC 11901 |
| title_sort | development of a biotechnology platform for the fast growing cyanobacterium em synechococcus em sp pcc 11901 |
| topic | <i>Synechococcus</i> sp. PCC 11901 CodA selection SacB selection vitamin B12 photosynthesis photoinhibition |
| url | https://www.mdpi.com/2218-273X/12/7/872 |
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