α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells
Glucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for glucose uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to th...
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MDPI AG
2024-01-01
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| Series: | International Journal of Molecular Sciences |
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| author | Giordana Feriotto Federico Tagliati Valentina Costa Marcello Monesi Claudio Tabolacci Simone Beninati Carlo Mischiati |
| author_facet | Giordana Feriotto Federico Tagliati Valentina Costa Marcello Monesi Claudio Tabolacci Simone Beninati Carlo Mischiati |
| author_sort | Giordana Feriotto |
| collection | DOAJ |
| description | Glucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for glucose uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to the cell plasma membrane is reduced, compromising cell energy metabolism. Therefore, the use of synthetic or naturally occurring molecules able to stimulate GLUT4 expression represents a good tool for alternative treatments of insulin resistance. The present study aimed to investigate the effects of essential oils (EOs) derived from <i>Pinus</i> spp. (<i>P. nigra</i> and <i>P. radiata</i>) and of their main terpenoid constituents (α- and β-pinene) on the expression/translocation of GLUT4 in myoblast C2C12 murine cells. For this purpose, the chemical profiles of the EOs were first analyzed through gas chromatography–mass spectrometry (GC-MS). Cell viability was assessed by MTT assay, and GLUT4 expression/translocation was evaluated through RT-qPCR and flow cytometry analyses. The results showed that only the <i>P. nigra</i> essential oil (PnEO) and α-pinene can increase the transcription of the <i>Glut4/Scl2a4</i> gene, resulting in a subsequent increase in the amount of GLUT4 produced and its plasma membrane localization. Moreover, the PnEO or α-pinene can induce <i>Glut4</i> expression both during myogenesis and in myotubes. In summary, the PnEO and α-pinene emulate insulin’s effect on the GLUT4 transporter expression and its translocation to the muscle cell surface. |
| first_indexed | 2024-03-08T09:52:40Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:52:40Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-38da238975c84ab8b43b088c5d5e22432024-01-29T13:58:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252125210.3390/ijms25021252α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle CellsGiordana Feriotto0Federico Tagliati1Valentina Costa2Marcello Monesi3Claudio Tabolacci4Simone Beninati5Carlo Mischiati6Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121 Ferrara, ItalyDepartment of Neurosciences and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyDepartment of Environmental Sciences and Prevention, University of Ferrara, 44121 Ferrara, ItalyUOC (Unità Operativa Complessa) Territorial Diabetology, AUSL Ferrara, 44121 Ferrara, ItalyResearch Coordination and Support Service, Superior Institute of Health, 00161 Rome, ItalyDepartment of Biology, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Neurosciences and Rehabilitation, University of Ferrara, 44121 Ferrara, ItalyGlucose transporter-4 (GLUT4) represents the major glucose transporter isoform responsible for glucose uptake into insulin-sensitive cells, primarily in skeletal muscle and adipose tissues. In insulin-resistant conditions, such as type 2 diabetes mellitus, GLUT4 expression and/or translocation to the cell plasma membrane is reduced, compromising cell energy metabolism. Therefore, the use of synthetic or naturally occurring molecules able to stimulate GLUT4 expression represents a good tool for alternative treatments of insulin resistance. The present study aimed to investigate the effects of essential oils (EOs) derived from <i>Pinus</i> spp. (<i>P. nigra</i> and <i>P. radiata</i>) and of their main terpenoid constituents (α- and β-pinene) on the expression/translocation of GLUT4 in myoblast C2C12 murine cells. For this purpose, the chemical profiles of the EOs were first analyzed through gas chromatography–mass spectrometry (GC-MS). Cell viability was assessed by MTT assay, and GLUT4 expression/translocation was evaluated through RT-qPCR and flow cytometry analyses. The results showed that only the <i>P. nigra</i> essential oil (PnEO) and α-pinene can increase the transcription of the <i>Glut4/Scl2a4</i> gene, resulting in a subsequent increase in the amount of GLUT4 produced and its plasma membrane localization. Moreover, the PnEO or α-pinene can induce <i>Glut4</i> expression both during myogenesis and in myotubes. In summary, the PnEO and α-pinene emulate insulin’s effect on the GLUT4 transporter expression and its translocation to the muscle cell surface.https://www.mdpi.com/1422-0067/25/2/1252glucose transporter-4essential oilsα-pinene |
| spellingShingle | Giordana Feriotto Federico Tagliati Valentina Costa Marcello Monesi Claudio Tabolacci Simone Beninati Carlo Mischiati α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells International Journal of Molecular Sciences glucose transporter-4 essential oils α-pinene |
| title | α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells |
| title_full | α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells |
| title_fullStr | α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells |
| title_full_unstemmed | α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells |
| title_short | α-Pinene, a Main Component of <i>Pinus</i> Essential Oils, Enhances the Expression of Insulin-Sensitive Glucose Transporter Type 4 in Murine Skeletal Muscle Cells |
| title_sort | α pinene a main component of i pinus i essential oils enhances the expression of insulin sensitive glucose transporter type 4 in murine skeletal muscle cells |
| topic | glucose transporter-4 essential oils α-pinene |
| url | https://www.mdpi.com/1422-0067/25/2/1252 |
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