An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans
The differential metabolite profiles of four wild and ten cultivated soybeans genotypes were explored using an untargeted metabolomics approach. Ground soybean seed samples were extracted with methanol and water, and metabolic features were obtained using ultra-high-performance liquid chromatography...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/14/5507 |
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| author | Fakir Shahidullah Tareq Raghavendhar R. Kotha Savithiry Natarajan Jianghao Sun Devanand L. Luthria |
| author_facet | Fakir Shahidullah Tareq Raghavendhar R. Kotha Savithiry Natarajan Jianghao Sun Devanand L. Luthria |
| author_sort | Fakir Shahidullah Tareq |
| collection | DOAJ |
| description | The differential metabolite profiles of four wild and ten cultivated soybeans genotypes were explored using an untargeted metabolomics approach. Ground soybean seed samples were extracted with methanol and water, and metabolic features were obtained using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) in both positive and negative ion modes. The UHPLC-HRMS analysis of the two different extracts resulted in the putative identification of 98 metabolites belonging to several classes of phytochemicals, including isoflavones, organic acids, lipids, sugars, amino acids, saponins, and other compounds. The metabolic profile was significantly impacted by the polarity of the extraction solvent. Multivariate analysis showed a clear difference between wild and cultivated soybean cultivars. Unsupervised and supervised learning algorithms were applied to mine the generated data and to pinpoint metabolites differentiating wild and cultivated soybeans. The key identified metabolites differentiating wild and cultivated soybeans were isoflavonoids, free amino acids, and fatty acids. Catechin analogs, cynaroside, hydroxylated unsaturated fatty acid derivatives, amino acid, and uridine diphosphate-<i>N</i>-acetylglucosamine were upregulated in the methanol extract of wild soybeans. In contrast, isoflavonoids and other minor compounds were downregulated in the same soybean extract. This metabolic information will benefit breeders and biotechnology professionals to develop value-added soybeans with improved quality traits. |
| first_indexed | 2024-03-11T00:46:44Z |
| format | Article |
| id | doaj.art-38dd636e1ab245a5a617a58f04996a05 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:46:44Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-38dd636e1ab245a5a617a58f04996a052023-11-18T20:43:08ZengMDPI AGMolecules1420-30492023-07-012814550710.3390/molecules28145507An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated SoybeansFakir Shahidullah Tareq0Raghavendhar R. Kotha1Savithiry Natarajan2Jianghao Sun3Devanand L. Luthria4Methods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USASoybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAMethods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USAThe differential metabolite profiles of four wild and ten cultivated soybeans genotypes were explored using an untargeted metabolomics approach. Ground soybean seed samples were extracted with methanol and water, and metabolic features were obtained using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) in both positive and negative ion modes. The UHPLC-HRMS analysis of the two different extracts resulted in the putative identification of 98 metabolites belonging to several classes of phytochemicals, including isoflavones, organic acids, lipids, sugars, amino acids, saponins, and other compounds. The metabolic profile was significantly impacted by the polarity of the extraction solvent. Multivariate analysis showed a clear difference between wild and cultivated soybean cultivars. Unsupervised and supervised learning algorithms were applied to mine the generated data and to pinpoint metabolites differentiating wild and cultivated soybeans. The key identified metabolites differentiating wild and cultivated soybeans were isoflavonoids, free amino acids, and fatty acids. Catechin analogs, cynaroside, hydroxylated unsaturated fatty acid derivatives, amino acid, and uridine diphosphate-<i>N</i>-acetylglucosamine were upregulated in the methanol extract of wild soybeans. In contrast, isoflavonoids and other minor compounds were downregulated in the same soybean extract. This metabolic information will benefit breeders and biotechnology professionals to develop value-added soybeans with improved quality traits.https://www.mdpi.com/1420-3049/28/14/5507soybean cultivarsmetabolomicsultra-high-performance liquid chromatography-tandem mass spectrometrymultivariate analysis |
| spellingShingle | Fakir Shahidullah Tareq Raghavendhar R. Kotha Savithiry Natarajan Jianghao Sun Devanand L. Luthria An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans Molecules soybean cultivars metabolomics ultra-high-performance liquid chromatography-tandem mass spectrometry multivariate analysis |
| title | An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans |
| title_full | An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans |
| title_fullStr | An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans |
| title_full_unstemmed | An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans |
| title_short | An Untargeted Metabolomics Approach to Study the Variation between Wild and Cultivated Soybeans |
| title_sort | untargeted metabolomics approach to study the variation between wild and cultivated soybeans |
| topic | soybean cultivars metabolomics ultra-high-performance liquid chromatography-tandem mass spectrometry multivariate analysis |
| url | https://www.mdpi.com/1420-3049/28/14/5507 |
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