PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation through cryo-focused ion beam (FIB) milling and...

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Bibliographic Details
Main Authors: Sergey Gorelick, Genevieve Buckley, Gediminas Gervinskas, Travis K Johnson, Ava Handley, Monica Pia Caggiano, James C Whisstock, Roger Pocock, Alex de Marco
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2019-07-01
Series:eLife
Subjects:
cryo-FIB
cryo-CLEM
cryo-LM
targeted cryo-FIB
correlative microscopy
in situ structural biology
Online Access:https://elifesciences.org/articles/45919

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