Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells
AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2013-06-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/ |
| _version_ | 1818977860078010368 |
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| author | Shi-Feng Zhou Qing Han Hong Zhang Lin Wang Guo-Dan Liu Ping Liu |
| author_facet | Shi-Feng Zhou Qing Han Hong Zhang Lin Wang Guo-Dan Liu Ping Liu |
| author_sort | Shi-Feng Zhou |
| collection | DOAJ |
| description | AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.RESULTS: The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.CONCLUSION: The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury. |
| first_indexed | 2024-12-20T16:34:27Z |
| format | Article |
| id | doaj.art-38e2bf1584b54b04b738251e53546350 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-20T16:34:27Z |
| publishDate | 2013-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-38e2bf1584b54b04b738251e535463502022-12-21T19:33:10ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982013-06-016328028510.3980/j.issn.2222-3959.2013.03.05Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cellsShi-Feng ZhouQing HanHong ZhangLin WangGuo-Dan LiuPing LiuAIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.RESULTS: The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.CONCLUSION: The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/hydrogenLipopolysaccharidesmiR-9miR-21miR-199Toll-like receptor 4 |
| spellingShingle | Shi-Feng Zhou Qing Han Hong Zhang Lin Wang Guo-Dan Liu Ping Liu Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells International Journal of Ophthalmology hydrogen Lipopolysaccharides miR-9 miR-21 miR-199 Toll-like receptor 4 |
| title | Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells |
| title_full | Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells |
| title_fullStr | Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells |
| title_full_unstemmed | Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells |
| title_short | Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells |
| title_sort | molecular hydrogen regulates the expression of mir 9 mir 21 and mir 199 in lps activated retinal microglia cells |
| topic | hydrogen Lipopolysaccharides miR-9 miR-21 miR-199 Toll-like receptor 4 |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693006/ |
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