RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause
During menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation of RANKL and FSHR genes were identified in 30 premenopausal and 35 postmenopausal women using methylation-specific high resolution melting (MS...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2020-08-01
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| Series: | Balkan Journal of Medical Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/bjmg-2020-0001 |
| _version_ | 1797718662350635008 |
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| author | Kalkan R Altarda M Tosun O |
| author_facet | Kalkan R Altarda M Tosun O |
| author_sort | Kalkan R |
| collection | DOAJ |
| description | During menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation of RANKL and FSHR genes were identified in 30 premenopausal and 35 postmenopausal women using methylation-specific high resolution melting (MS-HRM) analysis. The statistical analyses and their association with patient characteristics were performed by Pearson χ2 and Fisher’s exact test (p <0.05). The methylated RANKL gene was detected in 16 postmenopausal cases, and 12 (75.0%) of the RANKL methylated cases had hot flashes (p = 0.024). The methylated FSHR gene was detected in 18 postmenopausal cases, and 13 (75.0%) of the methylated cases had hot flashes (p = 0.028). In vitro studies demonstrated the association between RANKL expression, FSH level and hot flashes in the mouse. Although lack of epigenetic studies in this field proves our results crucial and therefore, our results showed magnitude of epigenetic profiles of Turkish Cypriot post-menopausal women. This was the first study which has investigated the RANKL and FSHR methylation and their relationship with hot flashes in postmenopausal women. |
| first_indexed | 2024-03-12T08:53:39Z |
| format | Article |
| id | doaj.art-38e2e853164648b992cafcdc4bbb68dd |
| institution | Directory Open Access Journal |
| issn | 1311-0160 |
| language | English |
| last_indexed | 2024-03-12T08:53:39Z |
| publishDate | 2020-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Balkan Journal of Medical Genetics |
| spelling | doaj.art-38e2e853164648b992cafcdc4bbb68dd2023-09-02T16:10:17ZengSciendoBalkan Journal of Medical Genetics1311-01602020-08-01231515610.2478/bjmg-2020-0001bjmg-2020-0001RANKL is a new epigenetic biomarker for the vasomotor symptom during MenopauseKalkan R0Altarda M1Tosun O2Department of Medical Genetics, Faculty of Medicine, Near East University, Nicosia, Turkish Republic of Northern CyprusDepartment of Medical Genetics, Faculty of Medicine, Near East University, Nicosia, Turkish Republic of Northern CyprusDepartment of Biostatistics, Faculty of Medicine, Near East University, Nicosia, Turkish Republic of Northern CyprusDuring menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation of RANKL and FSHR genes were identified in 30 premenopausal and 35 postmenopausal women using methylation-specific high resolution melting (MS-HRM) analysis. The statistical analyses and their association with patient characteristics were performed by Pearson χ2 and Fisher’s exact test (p <0.05). The methylated RANKL gene was detected in 16 postmenopausal cases, and 12 (75.0%) of the RANKL methylated cases had hot flashes (p = 0.024). The methylated FSHR gene was detected in 18 postmenopausal cases, and 13 (75.0%) of the methylated cases had hot flashes (p = 0.028). In vitro studies demonstrated the association between RANKL expression, FSH level and hot flashes in the mouse. Although lack of epigenetic studies in this field proves our results crucial and therefore, our results showed magnitude of epigenetic profiles of Turkish Cypriot post-menopausal women. This was the first study which has investigated the RANKL and FSHR methylation and their relationship with hot flashes in postmenopausal women.https://doi.org/10.2478/bjmg-2020-0001epigeneticsfshrrankl geneshot flashesmenopause |
| spellingShingle | Kalkan R Altarda M Tosun O RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause Balkan Journal of Medical Genetics epigenetics fshr rankl genes hot flashes menopause |
| title | RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause |
| title_full | RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause |
| title_fullStr | RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause |
| title_full_unstemmed | RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause |
| title_short | RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause |
| title_sort | rankl is a new epigenetic biomarker for the vasomotor symptom during menopause |
| topic | epigenetics fshr rankl genes hot flashes menopause |
| url | https://doi.org/10.2478/bjmg-2020-0001 |
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