Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation
The ciliate Paramecium bursaria harbors several hundred symbiotic algae in its cell and is widely used as an experimental model for studying symbiosis between eukaryotic cells. Currently, various types of bacteria and eukaryotic microorganisms are used as food for culturing P. bursaria; thus, the cu...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1036372/full |
| _version_ | 1811158651416608768 |
|---|---|
| author | Eiko Himi Tohru Miyoshi-Akiyama Yuri Matsushima Iru Shiono Seiji Aragane Yui Hirano Gaku Ikeda Yuki Kitaura Kyohei Kobayashi Daichi Konno Ayata Morohashi Yui Noguchi Yuka Ominato Soma Shinbo Naruya Suzuki Kurama Takatsuka Hitomi Tashiro Yoki Yamada Kenya Yamashita Natsumi Yoshino Masaharu Kitashima Susumu Kotani Susumu Kotani Susumu Kotani Kazuhito Inoue Kazuhito Inoue Kazuhito Inoue Akiya Hino Hiroshi Hosoya |
| author_facet | Eiko Himi Tohru Miyoshi-Akiyama Yuri Matsushima Iru Shiono Seiji Aragane Yui Hirano Gaku Ikeda Yuki Kitaura Kyohei Kobayashi Daichi Konno Ayata Morohashi Yui Noguchi Yuka Ominato Soma Shinbo Naruya Suzuki Kurama Takatsuka Hitomi Tashiro Yoki Yamada Kenya Yamashita Natsumi Yoshino Masaharu Kitashima Susumu Kotani Susumu Kotani Susumu Kotani Kazuhito Inoue Kazuhito Inoue Kazuhito Inoue Akiya Hino Hiroshi Hosoya |
| author_sort | Eiko Himi |
| collection | DOAJ |
| description | The ciliate Paramecium bursaria harbors several hundred symbiotic algae in its cell and is widely used as an experimental model for studying symbiosis between eukaryotic cells. Currently, various types of bacteria and eukaryotic microorganisms are used as food for culturing P. bursaria; thus, the cultivation conditions are not uniform among researchers. To unify cultivation conditions, we established cloned, unfed strains that can be cultured using only sterile medium without exogenous food. The proliferation of these unfed strains was suppressed in the presence of antibiotics, suggesting that bacteria are required for the proliferation of the unfed strains. Indeed, several kinds of bacteria, such as Burkholderiales, Rhizobiales, Rhodospirillales, and Sphingomonadales, which are able to fix atmospheric nitrogen and/or degrade chemical pollutants, were detected in the unfed strains. The genetic background of the individually cloned, unfed strains were the same, but the proliferation curves of the individual P. bursaria strains were very diverse. Therefore, we selected multiple actively and poorly proliferating individual strains and compared the bacterial composition among the individual strains using 16S rDNA sequencing. The results showed that the bacterial composition among actively proliferating P. bursaria strains was highly homologous but different to poorly proliferating strains. Using unfed strains, the cultivation conditions applied in different laboratories can be unified, and symbiosis research on P. bursaria will make great progress. |
| first_indexed | 2024-04-10T05:27:44Z |
| format | Article |
| id | doaj.art-38e584c738f948b3b4456f59fe14c8c0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T05:27:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-38e584c738f948b3b4456f59fe14c8c02023-03-07T12:29:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.10363721036372Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferationEiko Himi0Tohru Miyoshi-Akiyama1Yuri Matsushima2Iru Shiono3Seiji Aragane4Yui Hirano5Gaku Ikeda6Yuki Kitaura7Kyohei Kobayashi8Daichi Konno9Ayata Morohashi10Yui Noguchi11Yuka Ominato12Soma Shinbo13Naruya Suzuki14Kurama Takatsuka15Hitomi Tashiro16Yoki Yamada17Kenya Yamashita18Natsumi Yoshino19Masaharu Kitashima20Susumu Kotani21Susumu Kotani22Susumu Kotani23Kazuhito Inoue24Kazuhito Inoue25Kazuhito Inoue26Akiya Hino27Hiroshi Hosoya28Faculty of Agriculture, Kibi International University, Minamiawaji, Hyogo, JapanDepartment of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, JapanDepartment of Biological Sciences, Graduate School of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Graduate School of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanResearch Institute for Integrated Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Graduate School of Science, Kanagawa University, Kanagawa, JapanDepartment of Biological Sciences, Faculty of Science, Kanagawa University, Kanagawa, JapanResearch Institute for Integrated Science, Kanagawa University, Kanagawa, JapanResearch Institute for Integrated Science, Kanagawa University, Kanagawa, JapanResearch Institute for Integrated Science, Kanagawa University, Kanagawa, JapanThe ciliate Paramecium bursaria harbors several hundred symbiotic algae in its cell and is widely used as an experimental model for studying symbiosis between eukaryotic cells. Currently, various types of bacteria and eukaryotic microorganisms are used as food for culturing P. bursaria; thus, the cultivation conditions are not uniform among researchers. To unify cultivation conditions, we established cloned, unfed strains that can be cultured using only sterile medium without exogenous food. The proliferation of these unfed strains was suppressed in the presence of antibiotics, suggesting that bacteria are required for the proliferation of the unfed strains. Indeed, several kinds of bacteria, such as Burkholderiales, Rhizobiales, Rhodospirillales, and Sphingomonadales, which are able to fix atmospheric nitrogen and/or degrade chemical pollutants, were detected in the unfed strains. The genetic background of the individually cloned, unfed strains were the same, but the proliferation curves of the individual P. bursaria strains were very diverse. Therefore, we selected multiple actively and poorly proliferating individual strains and compared the bacterial composition among the individual strains using 16S rDNA sequencing. The results showed that the bacterial composition among actively proliferating P. bursaria strains was highly homologous but different to poorly proliferating strains. Using unfed strains, the cultivation conditions applied in different laboratories can be unified, and symbiosis research on P. bursaria will make great progress.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1036372/fullParamecium bursariasymbiosissymbiotic algaebacteriaunfed strainprotist |
| spellingShingle | Eiko Himi Tohru Miyoshi-Akiyama Yuri Matsushima Iru Shiono Seiji Aragane Yui Hirano Gaku Ikeda Yuki Kitaura Kyohei Kobayashi Daichi Konno Ayata Morohashi Yui Noguchi Yuka Ominato Soma Shinbo Naruya Suzuki Kurama Takatsuka Hitomi Tashiro Yoki Yamada Kenya Yamashita Natsumi Yoshino Masaharu Kitashima Susumu Kotani Susumu Kotani Susumu Kotani Kazuhito Inoue Kazuhito Inoue Kazuhito Inoue Akiya Hino Hiroshi Hosoya Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation Frontiers in Microbiology Paramecium bursaria symbiosis symbiotic algae bacteria unfed strain protist |
| title | Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| title_full | Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| title_fullStr | Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| title_full_unstemmed | Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| title_short | Establishment of an unfed strain of Paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| title_sort | establishment of an unfed strain of paramecium bursaria and analysis of associated bacterial communities controlling its proliferation |
| topic | Paramecium bursaria symbiosis symbiotic algae bacteria unfed strain protist |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1036372/full |
| work_keys_str_mv | AT eikohimi establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT tohrumiyoshiakiyama establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yurimatsushima establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT irushiono establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT seijiaragane establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yuihirano establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT gakuikeda establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yukikitaura establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kyoheikobayashi establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT daichikonno establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT ayatamorohashi establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yuinoguchi establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yukaominato establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT somashinbo establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT naruyasuzuki establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kuramatakatsuka establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT hitomitashiro establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT yokiyamada establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kenyayamashita establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT natsumiyoshino establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT masaharukitashima establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT susumukotani establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT susumukotani establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT susumukotani establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kazuhitoinoue establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kazuhitoinoue establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT kazuhitoinoue establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT akiyahino establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation AT hiroshihosoya establishmentofanunfedstrainofparameciumbursariaandanalysisofassociatedbacterialcommunitiescontrollingitsproliferation |