Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2
Due to the current, rapidly increasing Coronavirus disease 2019 (COVID-19) pandemic, efficient and highly specific diagnostic methods are needed. The receptor-binding part of the spike (S) protein, S1, has been suggested to be highly virus-specific; it does not cross-react with antibodies against ot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/6/993 |
| _version_ | 1797532514030452736 |
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| author | Tove Hoffman Linda Kolstad Johanna F. Lindahl Bo Albinsson Anders Bergqvist Bengt Rönnberg Åke Lundkvist |
| author_facet | Tove Hoffman Linda Kolstad Johanna F. Lindahl Bo Albinsson Anders Bergqvist Bengt Rönnberg Åke Lundkvist |
| author_sort | Tove Hoffman |
| collection | DOAJ |
| description | Due to the current, rapidly increasing Coronavirus disease 2019 (COVID-19) pandemic, efficient and highly specific diagnostic methods are needed. The receptor-binding part of the spike (S) protein, S1, has been suggested to be highly virus-specific; it does not cross-react with antibodies against other coronaviruses. Three recombinant partial S proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) expressed in mammalian or baculovirus-insect cells were evaluated as antigens in a Luminex-based suspension immunoassay (SIA). The best performing antigen (S1; amino acids 16-685) was selected and further evaluated by serum samples from 76 Swedish patients or convalescents with COVID-19 (previously PCR and/or serologically confirmed), 200 pre-COVID-19 individuals (180 blood donors and 20 infants), and 10 patients with acute Epstein-Barr virus infection. All 76 positive samples showed detectable antibodies to S1, while none of the 210 negative controls gave a false positive antibody reaction. We further compared the COVID-19 SIA with a commercially available enzyme immunoassay and a previously evaluated COVID-19 rapid antibody test. The results revealed an overall assay sensitivity of 100%, a specificity of 100% for both IgM and IgG, a quantitative ability at concentrations up to 25 BAU/mL, and a better performance as compared to the commercial assays, suggesting the COVID-19 SIA as a most valuable tool for efficient laboratory-based serology. |
| first_indexed | 2024-03-10T11:01:19Z |
| format | Article |
| id | doaj.art-38e5e8bd0fba4c109414bfd45df4a461 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T11:01:19Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-38e5e8bd0fba4c109414bfd45df4a4612023-11-21T21:29:17ZengMDPI AGViruses1999-49152021-05-0113699310.3390/v13060993Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2Tove Hoffman0Linda Kolstad1Johanna F. Lindahl2Bo Albinsson3Anders Bergqvist4Bengt Rönnberg5Åke Lundkvist6Department of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenLaboratory of Clinical Microbiology, Uppsala University Hospital, Dag Hammarskjölds väg 38, SE-752 37 Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenDepartment of Medical Biochemistry and Microbiology, Zoonosis Science Centre, Uppsala University, Husargatan 3, SE-751 23 Uppsala, SwedenDue to the current, rapidly increasing Coronavirus disease 2019 (COVID-19) pandemic, efficient and highly specific diagnostic methods are needed. The receptor-binding part of the spike (S) protein, S1, has been suggested to be highly virus-specific; it does not cross-react with antibodies against other coronaviruses. Three recombinant partial S proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) expressed in mammalian or baculovirus-insect cells were evaluated as antigens in a Luminex-based suspension immunoassay (SIA). The best performing antigen (S1; amino acids 16-685) was selected and further evaluated by serum samples from 76 Swedish patients or convalescents with COVID-19 (previously PCR and/or serologically confirmed), 200 pre-COVID-19 individuals (180 blood donors and 20 infants), and 10 patients with acute Epstein-Barr virus infection. All 76 positive samples showed detectable antibodies to S1, while none of the 210 negative controls gave a false positive antibody reaction. We further compared the COVID-19 SIA with a commercially available enzyme immunoassay and a previously evaluated COVID-19 rapid antibody test. The results revealed an overall assay sensitivity of 100%, a specificity of 100% for both IgM and IgG, a quantitative ability at concentrations up to 25 BAU/mL, and a better performance as compared to the commercial assays, suggesting the COVID-19 SIA as a most valuable tool for efficient laboratory-based serology.https://www.mdpi.com/1999-4915/13/6/993COVID-19SARS-CoV-2CoronavirusserologyIgMIgG |
| spellingShingle | Tove Hoffman Linda Kolstad Johanna F. Lindahl Bo Albinsson Anders Bergqvist Bengt Rönnberg Åke Lundkvist Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 Viruses COVID-19 SARS-CoV-2 Coronavirus serology IgM IgG |
| title | Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 |
| title_full | Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 |
| title_fullStr | Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 |
| title_full_unstemmed | Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 |
| title_short | Diagnostic Potential of a Luminex-Based Coronavirus Disease 2019 Suspension Immunoassay (COVID-19 SIA) for the Detection of Antibodies against SARS-CoV-2 |
| title_sort | diagnostic potential of a luminex based coronavirus disease 2019 suspension immunoassay covid 19 sia for the detection of antibodies against sars cov 2 |
| topic | COVID-19 SARS-CoV-2 Coronavirus serology IgM IgG |
| url | https://www.mdpi.com/1999-4915/13/6/993 |
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