On-chip on-demand delivery of K+ for in vitro bioelectronics
Bioelectronic devices that interface electronics with biological systems can actuate and control biological processes. The potassium ion plays a vital role in cell membrane physiology, maintaining the cell membrane potential (Vmem) and generating action potentials. In this work, we present two bioel...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2022-12-01
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| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/5.0129134 |
| _version_ | 1797947384877023232 |
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| author | Harika Dechiraju John Selberg Manping Jia Pattawong Pansodtee Houpu Li Hao-Chieh Hsieh Cristian Hernandez Narges Asefifeyzabadi Tiffany Nguyen Prabhat Baniya Giovanny Marquez Cody Rasmussen-Ivey Carrie Bradley Mircea Teodorescu Marcella Gomez Michael Levin Marco Rolandi |
| author_facet | Harika Dechiraju John Selberg Manping Jia Pattawong Pansodtee Houpu Li Hao-Chieh Hsieh Cristian Hernandez Narges Asefifeyzabadi Tiffany Nguyen Prabhat Baniya Giovanny Marquez Cody Rasmussen-Ivey Carrie Bradley Mircea Teodorescu Marcella Gomez Michael Levin Marco Rolandi |
| author_sort | Harika Dechiraju |
| collection | DOAJ |
| description | Bioelectronic devices that interface electronics with biological systems can actuate and control biological processes. The potassium ion plays a vital role in cell membrane physiology, maintaining the cell membrane potential (Vmem) and generating action potentials. In this work, we present two bioelectronic ion pumps that use an electronic signal to modulate the potassium ion concentration in solution. The first ion pump is designed to integrate directly with six-well cell culture plates for optimal ease of integration with in vitro cell culture, and the second on-chip ion pump provides high spatial resolution. These pumps offer increased ease of integration with in vitro systems and demonstrate K+ concentration distribution with high spatial resolution. We systematically investigate the ion pump’s performance using electrical characterization and computational modeling, and we explore closed-loop control of K+ concentration using fluorescent dyes as indicators. As a proof-of-concept, we study the effects of modulating K+ concentration on Vmem of THP-1 macrophages. |
| first_indexed | 2024-04-10T21:25:54Z |
| format | Article |
| id | doaj.art-38e86d1b33dd4570ab69a084c19718a3 |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-04-10T21:25:54Z |
| publishDate | 2022-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-38e86d1b33dd4570ab69a084c19718a32023-01-19T16:47:09ZengAIP Publishing LLCAIP Advances2158-32262022-12-011212125205125205-810.1063/5.0129134On-chip on-demand delivery of K+ for in vitro bioelectronicsHarika Dechiraju0John Selberg1Manping Jia2Pattawong Pansodtee3Houpu Li4Hao-Chieh Hsieh5Cristian Hernandez6Narges Asefifeyzabadi7Tiffany Nguyen8Prabhat Baniya9Giovanny Marquez10Cody Rasmussen-Ivey11Carrie Bradley12Mircea Teodorescu13Marcella Gomez14Michael Levin15Marco Rolandi16Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Applied Mathematics and Statistics, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Biology and Center for Regenerative and Developmental Biology, Tufts University, Medford, Massachusetts 02155, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Applied Mathematics and Statistics, University of California Santa Cruz, Santa Cruz, California 95060, USADepartment of Biology and Center for Regenerative and Developmental Biology, Tufts University, Medford, Massachusetts 02155, USADepartment of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, California 95060, USABioelectronic devices that interface electronics with biological systems can actuate and control biological processes. The potassium ion plays a vital role in cell membrane physiology, maintaining the cell membrane potential (Vmem) and generating action potentials. In this work, we present two bioelectronic ion pumps that use an electronic signal to modulate the potassium ion concentration in solution. The first ion pump is designed to integrate directly with six-well cell culture plates for optimal ease of integration with in vitro cell culture, and the second on-chip ion pump provides high spatial resolution. These pumps offer increased ease of integration with in vitro systems and demonstrate K+ concentration distribution with high spatial resolution. We systematically investigate the ion pump’s performance using electrical characterization and computational modeling, and we explore closed-loop control of K+ concentration using fluorescent dyes as indicators. As a proof-of-concept, we study the effects of modulating K+ concentration on Vmem of THP-1 macrophages.http://dx.doi.org/10.1063/5.0129134 |
| spellingShingle | Harika Dechiraju John Selberg Manping Jia Pattawong Pansodtee Houpu Li Hao-Chieh Hsieh Cristian Hernandez Narges Asefifeyzabadi Tiffany Nguyen Prabhat Baniya Giovanny Marquez Cody Rasmussen-Ivey Carrie Bradley Mircea Teodorescu Marcella Gomez Michael Levin Marco Rolandi On-chip on-demand delivery of K+ for in vitro bioelectronics AIP Advances |
| title | On-chip on-demand delivery of K+ for in vitro bioelectronics |
| title_full | On-chip on-demand delivery of K+ for in vitro bioelectronics |
| title_fullStr | On-chip on-demand delivery of K+ for in vitro bioelectronics |
| title_full_unstemmed | On-chip on-demand delivery of K+ for in vitro bioelectronics |
| title_short | On-chip on-demand delivery of K+ for in vitro bioelectronics |
| title_sort | on chip on demand delivery of k for in vitro bioelectronics |
| url | http://dx.doi.org/10.1063/5.0129134 |
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