Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation
Stroke is the second leading cause of death worldwide. Existing therapies present limitations, and other therapeutic alternatives are sought, such as sonothrombolysis with microbubbles (STL). The aim of this study was to evaluate the change induced by STL with or without recombinant tissue-type plas...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/13/10/1566 |
| _version_ | 1797513403660500992 |
|---|---|
| author | Laurent Auboire Damien Fouan Jean-Marc Grégoire Fréderic Ossant Camille Plag Jean-Michel Escoffre Ayache Bouakaz |
| author_facet | Laurent Auboire Damien Fouan Jean-Marc Grégoire Fréderic Ossant Camille Plag Jean-Michel Escoffre Ayache Bouakaz |
| author_sort | Laurent Auboire |
| collection | DOAJ |
| description | Stroke is the second leading cause of death worldwide. Existing therapies present limitations, and other therapeutic alternatives are sought, such as sonothrombolysis with microbubbles (STL). The aim of this study was to evaluate the change induced by STL with or without recombinant tissue-type plasminogen activator (rtPA) on the acoustic and elastic properties of the blood clot by measuring its sound speed (SoS) and shear wave speed (SWS) with high frequency ultrasound and ultrafast imaging, respectively. An in-vitro setup was used and human blood clots were submitted to a combination of microbubbles and rtPA. The results demonstrate that STL induces a raise of SoS in the blood clot, specifically when combined with rtPA (<i>p</i> < 0.05). Moreover, the combination of rtPA and STL induces a hardening of the clot in comparison to rtPA alone (<i>p</i> < 0.05). This is the first assessment of acoustoelastic properties of blood clots during STL. The combination of rtPA and STL induce SoS and hardening of the clot, which is known to impair the penetration of thrombolytic drugs and their efficacy. |
| first_indexed | 2024-03-10T06:17:01Z |
| format | Article |
| id | doaj.art-38e8f0a638ea4f8bb6062d535e871616 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:17:01Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-38e8f0a638ea4f8bb6062d535e8716162023-11-22T19:37:39ZengMDPI AGPharmaceutics1999-49232021-09-011310156610.3390/pharmaceutics13101566Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial CavitationLaurent Auboire0Damien Fouan1Jean-Marc Grégoire2Fréderic Ossant3Camille Plag4Jean-Michel Escoffre5Ayache Bouakaz6Imaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceImaging and Brain Unit (iBrain, Inserm Unit 1253), Université de Tours, 37000 Tours, FranceStroke is the second leading cause of death worldwide. Existing therapies present limitations, and other therapeutic alternatives are sought, such as sonothrombolysis with microbubbles (STL). The aim of this study was to evaluate the change induced by STL with or without recombinant tissue-type plasminogen activator (rtPA) on the acoustic and elastic properties of the blood clot by measuring its sound speed (SoS) and shear wave speed (SWS) with high frequency ultrasound and ultrafast imaging, respectively. An in-vitro setup was used and human blood clots were submitted to a combination of microbubbles and rtPA. The results demonstrate that STL induces a raise of SoS in the blood clot, specifically when combined with rtPA (<i>p</i> < 0.05). Moreover, the combination of rtPA and STL induces a hardening of the clot in comparison to rtPA alone (<i>p</i> < 0.05). This is the first assessment of acoustoelastic properties of blood clots during STL. The combination of rtPA and STL induce SoS and hardening of the clot, which is known to impair the penetration of thrombolytic drugs and their efficacy.https://www.mdpi.com/1999-4923/13/10/1566microbubblesstrokeultrasoundrtPAelastographyultrafast imaging |
| spellingShingle | Laurent Auboire Damien Fouan Jean-Marc Grégoire Fréderic Ossant Camille Plag Jean-Michel Escoffre Ayache Bouakaz Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation Pharmaceutics microbubbles stroke ultrasound rtPA elastography ultrafast imaging |
| title | Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation |
| title_full | Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation |
| title_fullStr | Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation |
| title_full_unstemmed | Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation |
| title_short | Acoustic and Elastic Properties of a Blood Clot during Microbubble-Enhanced Sonothrombolysis: Hardening of the Clot with Inertial Cavitation |
| title_sort | acoustic and elastic properties of a blood clot during microbubble enhanced sonothrombolysis hardening of the clot with inertial cavitation |
| topic | microbubbles stroke ultrasound rtPA elastography ultrafast imaging |
| url | https://www.mdpi.com/1999-4923/13/10/1566 |
| work_keys_str_mv | AT laurentauboire acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT damienfouan acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT jeanmarcgregoire acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT fredericossant acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT camilleplag acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT jeanmichelescoffre acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation AT ayachebouakaz acousticandelasticpropertiesofabloodclotduringmicrobubbleenhancedsonothrombolysishardeningoftheclotwithinertialcavitation |