Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
The honeybee <i>Apis mellifera</i> is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in...
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MDPI AG
2021-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/163 |
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| author | Ivana N. Macri Diego E. Vázquez Eduardo A. Pagano Jorge A. Zavala Walter M. Farina |
| author_facet | Ivana N. Macri Diego E. Vázquez Eduardo A. Pagano Jorge A. Zavala Walter M. Farina |
| author_sort | Ivana N. Macri |
| collection | DOAJ |
| description | The honeybee <i>Apis mellifera</i> is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxification in larvae and adult honeybees from three apiaries located in agricultural environments that differ in their proportion of the crop/wild flora. We evaluated these variables before and after the administration of a mixture of three herbicides during the summer season. The expression of several <i>cytochrome P450 monooxygenases</i> decreased significantly in larvae after post-emergence weed control and showed significant differences between apiaries in the case of honeybee workers. Principal component analysis (PCA) revealed that colonies located in the plot near to a wetland area exhibited a different relative gene expression profile after herbicide application compared with the other plots. Moreover, we found significant positive correlations between pollen collection and the pesticide detoxification genes that discriminated between plots in the PCA. Our results suggest that nutrition may modify herbicide impact on honeybees and that larvae are more harmed than adults in agroecosystems, a factor that will alter the colonies’ population growth at the end of the blooming period. |
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| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T00:53:21Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-38eacf5a8fb24fb79cd58e02c9784b1c2023-12-11T17:02:11ZengMDPI AGInsects2075-44502021-02-0112216310.3390/insects12020163Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural SurroundingsIvana N. Macri0Diego E. Vázquez1Eduardo A. Pagano2Jorge A. Zavala3Walter M. Farina4Laboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, ArgentinaLaboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, ArgentinaCátedra de Bioquímica, Facultad de Agronomía, Universidad de Buenos Aires, 1428 Buenos Aires, ArgentinaCátedra de Bioquímica, Facultad de Agronomía, Universidad de Buenos Aires, 1428 Buenos Aires, ArgentinaLaboratorio de Insectos Sociales, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, 1428 Buenos Aires, ArgentinaThe honeybee <i>Apis mellifera</i> is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxification in larvae and adult honeybees from three apiaries located in agricultural environments that differ in their proportion of the crop/wild flora. We evaluated these variables before and after the administration of a mixture of three herbicides during the summer season. The expression of several <i>cytochrome P450 monooxygenases</i> decreased significantly in larvae after post-emergence weed control and showed significant differences between apiaries in the case of honeybee workers. Principal component analysis (PCA) revealed that colonies located in the plot near to a wetland area exhibited a different relative gene expression profile after herbicide application compared with the other plots. Moreover, we found significant positive correlations between pollen collection and the pesticide detoxification genes that discriminated between plots in the PCA. Our results suggest that nutrition may modify herbicide impact on honeybees and that larvae are more harmed than adults in agroecosystems, a factor that will alter the colonies’ population growth at the end of the blooming period.https://www.mdpi.com/2075-4450/12/2/163<i>Apis mellifera</i>agricultural intensificationherbicides<i>cytochrome P450</i>pesticides detoxification |
| spellingShingle | Ivana N. Macri Diego E. Vázquez Eduardo A. Pagano Jorge A. Zavala Walter M. Farina Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings Insects <i>Apis mellifera</i> agricultural intensification herbicides <i>cytochrome P450</i> pesticides detoxification |
| title | Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings |
| title_full | Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings |
| title_fullStr | Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings |
| title_full_unstemmed | Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings |
| title_short | Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings |
| title_sort | evaluating the impact of post emergence weed control in honeybee colonies located in different agricultural surroundings |
| topic | <i>Apis mellifera</i> agricultural intensification herbicides <i>cytochrome P450</i> pesticides detoxification |
| url | https://www.mdpi.com/2075-4450/12/2/163 |
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