Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy
This study investigated the effectiveness of immobilizing <i>Saccharomyces cerevisiae</i> invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based on the existing literature and accompanied by parameter estimation, a modified kinetic model was employed...
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MDPI AG
2023-06-01
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Online Access: | https://www.mdpi.com/2305-7084/7/3/55 |
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author | David Polanía Melo Andrés Hernández Bravo Juan C. Cruz Luis H. Reyes |
author_facet | David Polanía Melo Andrés Hernández Bravo Juan C. Cruz Luis H. Reyes |
author_sort | David Polanía Melo |
collection | DOAJ |
description | This study investigated the effectiveness of immobilizing <i>Saccharomyces cerevisiae</i> invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based on the existing literature and accompanied by parameter estimation, a modified kinetic model was employed to represent the kinetics of sucrose hydrolysis and transfructosylation using SInv immobilized on magnetite nanoparticle surfaces. This model was utilized to simulate the performance of batch reactors for both free and immobilized enzymes. The maximum FOS concentration for the free enzyme was determined to be 123.1 mM, while the immobilized case achieved a slightly higher concentration of 125.4 mM. Furthermore, a continuous stirred-tank reactor (CSTR) model was developed for the immobilized enzyme, resulting in a maximum FOS concentration of 73.96 mM at the reactor’s outlet and a dilution rate of 14.2 h<sup>−1</sup>. To examine the impact of glucose inhibition on FOS production, a glucose oxidase reaction mechanism was integrated into the fitted immobilized theoretical model. In a batch reactor, the reduction or elimination of glucose in the reactive media led to a 2.1% increase in FOS production. Immobilizing the biocatalyst enhanced the overall performance of SInv. This enzyme immobilization approach also holds the potential for coupling glucose oxidase onto functionalized nanoparticles to minimize glucose inhibition, thereby improving FOS synthesis and facilitating optimal enzyme recovery and reuse. |
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spelling | doaj.art-38eba08adbef4114960dcd3d86ee26672023-11-18T09:45:09ZengMDPI AGChemEngineering2305-70842023-06-01735510.3390/chemengineering7030055Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design StrategyDavid Polanía Melo0Andrés Hernández Bravo1Juan C. Cruz2Luis H. Reyes3Product and Process Design Research Group (GDPP), Department of Chemical and Food Engineering, Universidad de Los Andes, Bogotá 111711, ColombiaProduct and Process Design Research Group (GDPP), Department of Chemical and Food Engineering, Universidad de Los Andes, Bogotá 111711, ColombiaProduct and Process Design Research Group (GDPP), Department of Chemical and Food Engineering, Universidad de Los Andes, Bogotá 111711, ColombiaProduct and Process Design Research Group (GDPP), Department of Chemical and Food Engineering, Universidad de Los Andes, Bogotá 111711, ColombiaThis study investigated the effectiveness of immobilizing <i>Saccharomyces cerevisiae</i> invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based on the existing literature and accompanied by parameter estimation, a modified kinetic model was employed to represent the kinetics of sucrose hydrolysis and transfructosylation using SInv immobilized on magnetite nanoparticle surfaces. This model was utilized to simulate the performance of batch reactors for both free and immobilized enzymes. The maximum FOS concentration for the free enzyme was determined to be 123.1 mM, while the immobilized case achieved a slightly higher concentration of 125.4 mM. Furthermore, a continuous stirred-tank reactor (CSTR) model was developed for the immobilized enzyme, resulting in a maximum FOS concentration of 73.96 mM at the reactor’s outlet and a dilution rate of 14.2 h<sup>−1</sup>. To examine the impact of glucose inhibition on FOS production, a glucose oxidase reaction mechanism was integrated into the fitted immobilized theoretical model. In a batch reactor, the reduction or elimination of glucose in the reactive media led to a 2.1% increase in FOS production. Immobilizing the biocatalyst enhanced the overall performance of SInv. This enzyme immobilization approach also holds the potential for coupling glucose oxidase onto functionalized nanoparticles to minimize glucose inhibition, thereby improving FOS synthesis and facilitating optimal enzyme recovery and reuse.https://www.mdpi.com/2305-7084/7/3/55fructooligosaccharidesinvertasenanoparticlesimmobilizationmodelingglucose inhibition |
spellingShingle | David Polanía Melo Andrés Hernández Bravo Juan C. Cruz Luis H. Reyes Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy ChemEngineering fructooligosaccharides invertase nanoparticles immobilization modeling glucose inhibition |
title | Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy |
title_full | Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy |
title_fullStr | Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy |
title_full_unstemmed | Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy |
title_short | Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy |
title_sort | invertase immobilization on magnetite nanoparticles for efficient fructooligosaccharide generation a comprehensive kinetic analysis and reactor design strategy |
topic | fructooligosaccharides invertase nanoparticles immobilization modeling glucose inhibition |
url | https://www.mdpi.com/2305-7084/7/3/55 |
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