Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium
Flower color variation in Chrysanthemum × morifolium is an important horticultural trait. This study identifies a natural bud sprout mutant of chrysanthemum cultivar Hanluhong (HLH) which normally produces red flowers. In the mutant (hlh), the tip of the petals turn golden yellow and gradually turn...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Maximum Academic Press
2022-01-01
|
| Series: | Ornamental Plant Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/OPR-2022-0019 |
| _version_ | 1827339865888391168 |
|---|---|
| author | Sagheer Ahmad Cunquan Yuan Tianci Cong Qingqing Yang Yujie Yang Qixiang Zhang |
| author_facet | Sagheer Ahmad Cunquan Yuan Tianci Cong Qingqing Yang Yujie Yang Qixiang Zhang |
| author_sort | Sagheer Ahmad |
| collection | DOAJ |
| description | Flower color variation in Chrysanthemum × morifolium is an important horticultural trait. This study identifies a natural bud sprout mutant of chrysanthemum cultivar Hanluhong (HLH) which normally produces red flowers. In the mutant (hlh), the tip of the petals turn golden yellow and gradually turn yellow from tip to the base. After two years of consecutive propagation through cuttings, the mutant traits were stable. Here, the contents of carotenoids and anthocyanidins were determined by HPLC-MS/MS. Compared to HLH, the hlh displayed significantly higher contents of lutein. Then we comparatively analyzed the transcriptome of the ray florets tissues during three flower developmental stages. Higher number of stage specific DEGs were found in mutant cultivar as compared to wild type. About 200 DEGs were filtered related to the metabolism of flavonoids, carotenoids, terpenoids, and anthocyanins. Combined with weighted gene co-expression network analysis (WGCNA) and qRT-PCR verification, CHI, DFR, ANS and CCD4 genes involved in anthocyanins biosynthesis and carotenoids degradation were identified as candidate genes. Among the transcription factors, MYB, bHLH and WD40 members showed significant expression variations between the two cultivars. These results improve our understanding of flower color variation especially red and yellow color transition in chrysanthemum. |
| first_indexed | 2024-03-07T20:07:45Z |
| format | Article |
| id | doaj.art-38ec514eb496437eb7324b88f2cfbf1b |
| institution | Directory Open Access Journal |
| issn | 2769-2094 |
| language | English |
| last_indexed | 2024-03-07T20:07:45Z |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj.art-38ec514eb496437eb7324b88f2cfbf1b2024-02-28T03:46:01ZengMaximum Academic PressOrnamental Plant Research2769-20942022-01-012111210.48130/OPR-2022-0019OPR-2022-0019Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifoliumSagheer Ahmad0Cunquan Yuan1Tianci Cong2Qingqing Yang3Yujie Yang4Qixiang Zhang5Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaBeijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Engineering Research Center of Landscape Environment of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, ChinaFlower color variation in Chrysanthemum × morifolium is an important horticultural trait. This study identifies a natural bud sprout mutant of chrysanthemum cultivar Hanluhong (HLH) which normally produces red flowers. In the mutant (hlh), the tip of the petals turn golden yellow and gradually turn yellow from tip to the base. After two years of consecutive propagation through cuttings, the mutant traits were stable. Here, the contents of carotenoids and anthocyanidins were determined by HPLC-MS/MS. Compared to HLH, the hlh displayed significantly higher contents of lutein. Then we comparatively analyzed the transcriptome of the ray florets tissues during three flower developmental stages. Higher number of stage specific DEGs were found in mutant cultivar as compared to wild type. About 200 DEGs were filtered related to the metabolism of flavonoids, carotenoids, terpenoids, and anthocyanins. Combined with weighted gene co-expression network analysis (WGCNA) and qRT-PCR verification, CHI, DFR, ANS and CCD4 genes involved in anthocyanins biosynthesis and carotenoids degradation were identified as candidate genes. Among the transcription factors, MYB, bHLH and WD40 members showed significant expression variations between the two cultivars. These results improve our understanding of flower color variation especially red and yellow color transition in chrysanthemum.https://www.maxapress.com/article/doi/10.48130/OPR-2022-0019color mutantchrysanthemumcarotenoidsanthocyanins |
| spellingShingle | Sagheer Ahmad Cunquan Yuan Tianci Cong Qingqing Yang Yujie Yang Qixiang Zhang Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium Ornamental Plant Research color mutant chrysanthemum carotenoids anthocyanins |
| title | Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium |
| title_full | Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium |
| title_fullStr | Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium |
| title_full_unstemmed | Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium |
| title_short | Transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of Chrysanthemum × morifolium |
| title_sort | transcriptome and chemical analyses identify candidate genes associated with flower color shift in a natural mutant of chrysanthemum morifolium |
| topic | color mutant chrysanthemum carotenoids anthocyanins |
| url | https://www.maxapress.com/article/doi/10.48130/OPR-2022-0019 |
| work_keys_str_mv | AT sagheerahmad transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium AT cunquanyuan transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium AT tiancicong transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium AT qingqingyang transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium AT yujieyang transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium AT qixiangzhang transcriptomeandchemicalanalysesidentifycandidategenesassociatedwithflowercolorshiftinanaturalmutantofchrysanthemummorifolium |