Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet
Background: Colonic microbiome is thought to be involved in auto-immune multiple sclerosis (MS). Interactions between diet and the colonic microbiome in MS are unknown.Methods: We compared the composition of the colonic microbiota quantitatively in 25 MS patients and 14 healthy controls.Fluorescence...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01141/full |
| _version_ | 1818841555130122240 |
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| author | Alexander Swidsinski Alexander Swidsinski Yvonne Dörffel Vera Loening-Baucke Christoph Gille Önder Göktas Anne Reißhauer Jürgen Neuhaus Karsten-Henrich Weylandt Alexander Guschin Markus Bock |
| author_facet | Alexander Swidsinski Alexander Swidsinski Yvonne Dörffel Vera Loening-Baucke Christoph Gille Önder Göktas Anne Reißhauer Jürgen Neuhaus Karsten-Henrich Weylandt Alexander Guschin Markus Bock |
| author_sort | Alexander Swidsinski |
| collection | DOAJ |
| description | Background: Colonic microbiome is thought to be involved in auto-immune multiple sclerosis (MS). Interactions between diet and the colonic microbiome in MS are unknown.Methods: We compared the composition of the colonic microbiota quantitatively in 25 MS patients and 14 healthy controls.Fluorescence in situ hybridization (FISH) with 162 ribosomal RNA derived bacterial FISH probes was used. Ten of the MS patients received a ketogenic diet for 6 months. Changes in concentrations of 35 numerically substantial bacterial groups were monitored at baseline and at 2, 12, and 23/24 weeks.Results: No MS typical microbiome pattern was apparent.The total concentrations and diversity of substantial bacterial groups were reduced in MS patients (P < 0.001). Bacterial groups detected with EREC (mainly Roseburia), Bac303 (Bacteroides), and Fprau (Faecalibacterium prausnitzii) probes were diminished the most. The individual changes were multidirectional and inconsistent. The effects of a ketogenic diet were biphasic. In the short term, bacterial concentrations and diversity were further reduced. They started to recover at week 12 and exceeded significantly the baseline values after 23–24 weeks on the ketogenic diet.Conclusions: Colonic biofermentative function is markedly impaired in MS patients.The ketogenic diet normalized concentrations of the colonic microbiome after 6 months. |
| first_indexed | 2024-12-19T04:27:56Z |
| format | Article |
| id | doaj.art-38ecd4cc04f74be28d31eb1857d25a06 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T04:27:56Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-38ecd4cc04f74be28d31eb1857d25a062022-12-21T20:35:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-06-01810.3389/fmicb.2017.01141283641Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic DietAlexander Swidsinski0Alexander Swidsinski1Yvonne Dörffel2Vera Loening-Baucke3Christoph Gille4Önder Göktas5Anne Reißhauer6Jürgen Neuhaus7Karsten-Henrich Weylandt8Alexander Guschin9Markus Bock10Laboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Section of Gastroenterology and Hepatology, Department of Medicine, Charité Universitätsmedizin BerlinBerlin, GermanyInstitute of Molecular Medicine, Sechenov First Moscow State Medical UniversityMoscow, RussiaOutpatient Clinic, Charité Universitätsmedizin BerlinBerlin, GermanyLaboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Section of Gastroenterology and Hepatology, Department of Medicine, Charité Universitätsmedizin BerlinBerlin, GermanyLaboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Section of Gastroenterology and Hepatology, Department of Medicine, Charité Universitätsmedizin BerlinBerlin, GermanyLaboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Section of Gastroenterology and Hepatology, Department of Medicine, Charité Universitätsmedizin BerlinBerlin, GermanyLaboratory for Molecular Genetics, Polymicrobial Infections and Biofilms, Section of Gastroenterology and Hepatology, Department of Medicine, Charité Universitätsmedizin BerlinBerlin, GermanyFaculty of Veterinary Medicine, Centre for Infectious Diseases, University of LeipzigLeipzig, GermanyCharité Universitätsmedizin Berlin, Campus Virchow, Gastroenterology BerlinBerlin, GermanyLaboratory of Molecular Diagnostics, Russian Research Center for Molecular Diagnostics and Therapy, Central Research InstituteMoscow, RussiaExperimental and Clinical Research Center, A Joint Cooperation between the Charité Medical Faculty and the Max-Delbrueck Center for Molecular MedicineBerlin, GermanyBackground: Colonic microbiome is thought to be involved in auto-immune multiple sclerosis (MS). Interactions between diet and the colonic microbiome in MS are unknown.Methods: We compared the composition of the colonic microbiota quantitatively in 25 MS patients and 14 healthy controls.Fluorescence in situ hybridization (FISH) with 162 ribosomal RNA derived bacterial FISH probes was used. Ten of the MS patients received a ketogenic diet for 6 months. Changes in concentrations of 35 numerically substantial bacterial groups were monitored at baseline and at 2, 12, and 23/24 weeks.Results: No MS typical microbiome pattern was apparent.The total concentrations and diversity of substantial bacterial groups were reduced in MS patients (P < 0.001). Bacterial groups detected with EREC (mainly Roseburia), Bac303 (Bacteroides), and Fprau (Faecalibacterium prausnitzii) probes were diminished the most. The individual changes were multidirectional and inconsistent. The effects of a ketogenic diet were biphasic. In the short term, bacterial concentrations and diversity were further reduced. They started to recover at week 12 and exceeded significantly the baseline values after 23–24 weeks on the ketogenic diet.Conclusions: Colonic biofermentative function is markedly impaired in MS patients.The ketogenic diet normalized concentrations of the colonic microbiome after 6 months.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01141/fullFISHcolonic microbiotamultiple sclerosisbiofermentationketogenic diet |
| spellingShingle | Alexander Swidsinski Alexander Swidsinski Yvonne Dörffel Vera Loening-Baucke Christoph Gille Önder Göktas Anne Reißhauer Jürgen Neuhaus Karsten-Henrich Weylandt Alexander Guschin Markus Bock Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet Frontiers in Microbiology FISH colonic microbiota multiple sclerosis biofermentation ketogenic diet |
| title | Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet |
| title_full | Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet |
| title_fullStr | Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet |
| title_full_unstemmed | Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet |
| title_short | Reduced Mass and Diversity of the Colonic Microbiome in Patients with Multiple Sclerosis and Their Improvement with Ketogenic Diet |
| title_sort | reduced mass and diversity of the colonic microbiome in patients with multiple sclerosis and their improvement with ketogenic diet |
| topic | FISH colonic microbiota multiple sclerosis biofermentation ketogenic diet |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01141/full |
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