Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice
Introduction: Anxiety disorders being ranked at sixth position in the global burden of diseases is affecting over 250 million people. Curcumin, an active phytochemical flavonoid, has shown to induce the monoamine neurotransmitter serotonin, a prominent neurotransmitter in modulating the brain st...
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JCDR Research and Publications Private Limited
2020-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/14157/45405_CE[Ra1]_F(SHU)_PF1(AB_SL)_PFA(AB_SL)_PN(SL).pdf |
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| author | ISSWARIYA ANANDAN NITYA SELVARAJ R MEENAKSHI MEHER ALI RAJAMOHAMMAD NISHANTHI ANANDABASKAR NALINI DEVI JAYABALAN |
| author_facet | ISSWARIYA ANANDAN NITYA SELVARAJ R MEENAKSHI MEHER ALI RAJAMOHAMMAD NISHANTHI ANANDABASKAR NALINI DEVI JAYABALAN |
| author_sort | ISSWARIYA ANANDAN |
| collection | DOAJ |
| description | Introduction: Anxiety disorders being ranked at sixth position
in the global burden of diseases is affecting over 250 million
people. Curcumin, an active phytochemical flavonoid, has
shown to induce the monoamine neurotransmitter serotonin,
a prominent neurotransmitter in modulating the brain state in
anxiety. Also, evidences reveal that zinc plays a key role in human
neurodevelopment and supplementation of zinc enhanced the
efficacy of antidepressant drugs through synergistic action.
Aim: To evaluate the synergistic antianxiety effect of curcumin
and zinc on acute and chronic models of anxiety in male swiss
albino mice.
Materials and Methods: A total of 36 male Swiss Albino mice,
weighing 20-30 g, were randomly grouped to six groups, such
that each group consisted of six mice. Group 1 served as
control. Group 2 received standard drug diazepam 3 mg/kg Intra
Peritoneal (IP). Group 3 and 4 received curcumin at doses of 5
and 10 mg/kg, respectively. Group 5 and 6 received curcumin
at doses 5 and 10 mg/kg per oral (p.o) along with zinc chloride
10 mg/kg IP, respectively. The anxiolytic effect was studied in two
validated models of anxiety such as Elevated Plus Maze (EPM)
test and light/dark box test. Each animal was tested initially in
the EPM followed by light/dark box test after administration of
drug/vehicle one hour prior to the experiment in acute study.
Following a washout period of one week, the animals were utilised
for the study of chronic anxiolytic effect wherein the drugs were
administered once daily for 14 days.
Results: Curcumin at doses of 5 mg/kg and 10 mg/kg with zinc
chloride 10 mg/kg showed a significant increase in the number
of entries and time spent in open arm in EPM both on acute
and chronic administration (p<0.001). In the light/dark box test,
curcumin at doses of 5 mg/kg and 10 mg/kg when given along
with zinc chloride 10 mg/kg significantly increased the number
of entries and time spent in the light compartment both in acute
and chronic models (p<0.001).
Conclusion: The anxiolytic effect of synergistic action of
curcumin and zinc was efficacious in both acute and chronic
models of anxiety in mice. |
| first_indexed | 2024-12-24T01:10:41Z |
| format | Article |
| id | doaj.art-38ed625db5f344b995b1901142a2fa8c |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-24T01:10:41Z |
| publishDate | 2020-10-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-38ed625db5f344b995b1901142a2fa8c2022-12-21T17:22:59ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2020-10-011410FC08FC1110.7860/JCDR/2020/45405.14157Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in MiceISSWARIYA ANANDAN0NITYA SELVARAJ1R MEENAKSHI2MEHER ALI RAJAMOHAMMAD3NISHANTHI ANANDABASKAR4NALINI DEVI JAYABALAN5Postgraduate, Department of Pharmacology, SRI Manakula Vinayagar Medical College and Hospital, Pondicherry, India.Associate Professor, Department of Pharmacology, SRI Manakula Vinayagar Medical College and Hospital, Pondicherry, India.Postgraduate, Department of Pharmacology, SRI Manakula Vinayagar Medical College and Hospital, Pondicherry, India.Professor, Department of Pharmacology, SRI Manakula Vinayagar Medical College and Hospital, Pondicherry, India.Assistant Professor, Department of Pharmacology, SRI Manakula Vinayagar Medical College and Hospital, Pondicherry, India.Clinical Iinvestigator, Department of Pharmacology, IC Bio Clinical Research Pvt. Ltd., Bengaluru, Karnataka, India.Introduction: Anxiety disorders being ranked at sixth position in the global burden of diseases is affecting over 250 million people. Curcumin, an active phytochemical flavonoid, has shown to induce the monoamine neurotransmitter serotonin, a prominent neurotransmitter in modulating the brain state in anxiety. Also, evidences reveal that zinc plays a key role in human neurodevelopment and supplementation of zinc enhanced the efficacy of antidepressant drugs through synergistic action. Aim: To evaluate the synergistic antianxiety effect of curcumin and zinc on acute and chronic models of anxiety in male swiss albino mice. Materials and Methods: A total of 36 male Swiss Albino mice, weighing 20-30 g, were randomly grouped to six groups, such that each group consisted of six mice. Group 1 served as control. Group 2 received standard drug diazepam 3 mg/kg Intra Peritoneal (IP). Group 3 and 4 received curcumin at doses of 5 and 10 mg/kg, respectively. Group 5 and 6 received curcumin at doses 5 and 10 mg/kg per oral (p.o) along with zinc chloride 10 mg/kg IP, respectively. The anxiolytic effect was studied in two validated models of anxiety such as Elevated Plus Maze (EPM) test and light/dark box test. Each animal was tested initially in the EPM followed by light/dark box test after administration of drug/vehicle one hour prior to the experiment in acute study. Following a washout period of one week, the animals were utilised for the study of chronic anxiolytic effect wherein the drugs were administered once daily for 14 days. Results: Curcumin at doses of 5 mg/kg and 10 mg/kg with zinc chloride 10 mg/kg showed a significant increase in the number of entries and time spent in open arm in EPM both on acute and chronic administration (p<0.001). In the light/dark box test, curcumin at doses of 5 mg/kg and 10 mg/kg when given along with zinc chloride 10 mg/kg significantly increased the number of entries and time spent in the light compartment both in acute and chronic models (p<0.001). Conclusion: The anxiolytic effect of synergistic action of curcumin and zinc was efficacious in both acute and chronic models of anxiety in mice.https://jcdr.net/articles/PDF/14157/45405_CE[Ra1]_F(SHU)_PF1(AB_SL)_PFA(AB_SL)_PN(SL).pdfantianxiety agentselevated plus mazelight dark box |
| spellingShingle | ISSWARIYA ANANDAN NITYA SELVARAJ R MEENAKSHI MEHER ALI RAJAMOHAMMAD NISHANTHI ANANDABASKAR NALINI DEVI JAYABALAN Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice Journal of Clinical and Diagnostic Research antianxiety agents elevated plus maze light dark box |
| title | Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice |
| title_full | Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice |
| title_fullStr | Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice |
| title_full_unstemmed | Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice |
| title_short | Synergistic Anxiolytic Effect of Curcumin and Zinc on Acute and Chronic Models of Anxiety in Mice |
| title_sort | synergistic anxiolytic effect of curcumin and zinc on acute and chronic models of anxiety in mice |
| topic | antianxiety agents elevated plus maze light dark box |
| url | https://jcdr.net/articles/PDF/14157/45405_CE[Ra1]_F(SHU)_PF1(AB_SL)_PFA(AB_SL)_PN(SL).pdf |
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