Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group
Premise Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 marke...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-11-01
|
| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11303 |
| _version_ | 1819044907537399808 |
|---|---|
| author | Jeremie B. Fant Mereida Fluckes Evana James Hilary Noble Jordan Wood |
| author_facet | Jeremie B. Fant Mereida Fluckes Evana James Hilary Noble Jordan Wood |
| author_sort | Jeremie B. Fant |
| collection | DOAJ |
| description | Premise Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross‐species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers. Conclusions Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group. |
| first_indexed | 2024-12-21T10:20:08Z |
| format | Article |
| id | doaj.art-38f3cee993924a8db63fd0aac132191a |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-21T10:20:08Z |
| publishDate | 2019-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-38f3cee993924a8db63fd0aac132191a2022-12-21T19:07:27ZengWileyApplications in Plant Sciences2168-04502019-11-01711n/an/a10.1002/aps3.11303Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid groupJeremie B. Fant0Mereida Fluckes1Evana James2Hilary Noble3Jordan Wood4Plant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USAPlant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USAPlant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USAPlant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USAPlant Science and Conservation Chicago Botanic Garden 1000 Lake Cook Road Glencoe Illinois 60022 USAPremise Microsatellite markers were developed to measure genetic diversity and relatedness of ex situ collections of Brighamia insignis (Campanulaceae). Methods and Results Potential microsatellite markers were identified from two sources; 28 were developed for B. insignis and an additional 12 markers from a previously published study of Lobelia villosa. Primer pairs were tested on 30 individuals of B. insignis and 24 individuals of B. rockii to provide measures of genetic diversity and inbreeding. We assessed cross‐species amplification in an additional 13 taxa that represented all six genera within the Hawai‘ian lobelioid group to determine the broader applicability of the markers. Conclusions Results indicate that these primers will provide useful estimates of genetic diversity and relatedness of ex situ collections of both Brighamia species. In addition, we have also demonstrated the widespread applicability of these markers for use in population genetic studies of several species within the Hawai‘ian lobelioid group.https://doi.org/10.1002/aps3.11303BrighamiaCampanulaceaecross‐amplificationHawai‘ian lobelioidsLobeliamicrosatellites |
| spellingShingle | Jeremie B. Fant Mereida Fluckes Evana James Hilary Noble Jordan Wood Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group Applications in Plant Sciences Brighamia Campanulaceae cross‐amplification Hawai‘ian lobelioids Lobelia microsatellites |
| title | Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group |
| title_full | Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group |
| title_fullStr | Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group |
| title_full_unstemmed | Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group |
| title_short | Characterization of microsatellite loci in Brighamia insignis and transferability to other genera in the Hawai‘ian lobelioid group |
| title_sort | characterization of microsatellite loci in brighamia insignis and transferability to other genera in the hawai ian lobelioid group |
| topic | Brighamia Campanulaceae cross‐amplification Hawai‘ian lobelioids Lobelia microsatellites |
| url | https://doi.org/10.1002/aps3.11303 |
| work_keys_str_mv | AT jeremiebfant characterizationofmicrosatellitelociinbrighamiainsignisandtransferabilitytoothergenerainthehawaiianlobelioidgroup AT mereidafluckes characterizationofmicrosatellitelociinbrighamiainsignisandtransferabilitytoothergenerainthehawaiianlobelioidgroup AT evanajames characterizationofmicrosatellitelociinbrighamiainsignisandtransferabilitytoothergenerainthehawaiianlobelioidgroup AT hilarynoble characterizationofmicrosatellitelociinbrighamiainsignisandtransferabilitytoothergenerainthehawaiianlobelioidgroup AT jordanwood characterizationofmicrosatellitelociinbrighamiainsignisandtransferabilitytoothergenerainthehawaiianlobelioidgroup |